RI SATURN 5, Руководство пользователя

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Section 8
8
33
Saturn 5™ Laser Operation
Research Instruments Ltd
How to Start Up
There is no power button for the Saturn 5. Once the device has been plugged into the power outlet and
the power outlet is switched on then the device is active and can be used.
1. Plug the device into the power outlet.
2. Ensure outlet is switched on.
How to Configure the Laser for a New Procedure
1. Check objective calibration, see page 20.
2. Check laser target alignment, see below.
3. Check hole size calibration, see page 34 .
How to Check Laser Target Alignment for Saturn 5 Active Laser System
If the laser has not been aligned before then when you start RI Viewer the alignment procedure will
start automatically.
Laser alignment of the Saturn 5 Active Laser System can be checked by following these steps.
1. Click the
Laser Control
icon.
2. Click Laser Settings.
3. Click Align Laser.
4. Ensure there are no embryos in the field of view.
5. Using an empty Petri dish or a slide adjust the focus of the pilot laser to ensure the pilot laser
spot is as small as possible. The brightness of the pilot laser can be adjusted.
6. Click Align Laser.
7. The motor will carry out a 7-point alignment at various points on the screen. At each motor
position click the screen at the exact centre of the pilot laser.
8. Click Next to proceed to the next alignment point.
9. When the final alignment point is finished, click on the screen to drive the motor to the selected
point and test the alignment by looking at the distance between the selected point and the
actual position of the pilot laser. If the alignment is acceptable press Finish to close the alignment
window and turn off the pilot laser, or press Align Laser to repeat the process.
10. The laser positioning is accurate to 1μm or better within the area
bounded by the four outer calibration points (see diagram on the
right). For optimum use of the laser, place the section of your
specimen to be ablated within this area. Outside this area the
accuracy is not guaranteed to be better than 3μm.
The diagram (right) represents the 7 calibration points on the screen.
The exact position of the points on the screen will vary depending on
the field of view and on the individual microscope.
SECTION 8 - LASER OPERATION