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XCell™ C410:V3 Controller
User Guide
43 repligen.com UG-C410v3-06
fiber. There are different preparation methods depending on the module type purchased. Two types
of HFMs are available for the XCell ATF® Device; accordingly, two types of Filter Housings are
available:
•
S-line Housing for use with HFM with “O” rings on the HFM ends for sealing against the
housing inner ID.
•
I-line Housing for use with HFM with no attached “O” rings. The “O” ring is placed in a recess
at the housing ends, between HFM and Reducer (or diaphragm pump). Clamping the
connection between reducer (or diaphragm pump) and the filter housing squeezes the “O”
ring effectively forcing the “O” ring against the filter wall, effectively sealing the three parts.
Figure 22. Types of filter housings
4.3.1
S-Line HFM insertion
1.
Lay the filter housing horizontally and insert HFM (without “O” rings) into the Filter
Housing.
Step 2 is to avoid damage to the “O” ring during its insertion and sliding into the housing, to avoid
crossing the harvest port opening during insertion, as the Drain port is close to the housing end.
2.
Expose HFM at the filter housing end distant to Drain port, in proximity to the
filtrate/harvest port.
3.
Place an “O” ring in the exposed HFM “O” ring groove.
4.
Press the HFM with “O” ring firmly into the housing.
Assure the “O” ring is inserted smoothly and uniformly. Lubricate with WFI to facilitate this process.
5.
Press until the HFM “O” ring groove is exposed at the other end, the Drain nozzle end.
6.
Place 2
nd
“O” ring on the exposed end.
7.
Press the HFM back into the filter housing until both “O” rings sealed against the housing ID.
8.
Assemble the system.
4.3.2
I-Line HFM insertion
1.
Stand the filter housing vertically, with harvest port up.
2.
Insert the HFM into the housing.
3.
Insert one “O” ring into the groove formed between HFM and filter housing end-ferrule wall.
4.
Place the reducer onto the end, forcing the “O” ring into the groove.
5.
Clamp the reducer to the housing, the compressed “O” ring is forced against the HFM. A seal
is formed between the housing, reducer and HFM.
6.
On the other end of the filter housing, place the second “O” ring into the formed groove
between HFM and housing end-ferrule.
7.
Carefully place the filter housing onto the diaphragm pump, forcing the “O” ring deeper into
the groove.
8.
Clamp the filter housing to the diaphragm pump.