REPLIGEN TangenX SIUS PD, Руководство пользователя

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TangenX TM SIUS TM PD Filter Plate & 2-Bolt Clamp Assembly
User Guide
Identification IF.PUG.019
Revision R2
Approved
20-DEC-2018
Using the calibrated torque wrench with a 11/16” deep style socket, tighten each hex nut ¼
turn following the torque sequence illustrated below.
Figure 7.1 Torque sequence
The applied torque should fall between the minimum value 120 in-lbs and the maximum value
of 180 in-lbs . Place the socket on bolt "1" and tighten the nut ¼ turn. Then move the wrench
to bolt "2" and tighten the nut ¼ turn. Alternate back and forth between bolts “1” and “2” until
the torque wrench "clicks" at each nut. Repeat this sequence until the wrench "clicks" without
turning the nut. The "click" of the torque wrench indicates that the nut has reached the set
point torque value.
CAUTION: Nuts must be tightened uniformly to avoid damaging the cassette. Leakage
may result from non-parallel plate alignment or over compression of the
cassettes at one end.
6. Wait 5-10 minutes and allow the gaskets to relax before re-torquing. Check each nut, per
Figure 7.1 sequence, using the torque wrench at its set point torque value.
7. Re-torque as needed to create a liquid-tight seal, but do not exceed the maximum torque limit
for the 4BMC clamp.
WARNING: The hex nuts should be checked for adequate torque PRIOR to every run using
the procedure described herein. In addition, torque may change during
processing as the cassettes may expand or contract with temperature changes.
Periodically check the torque of each nut and adjust as needed.
8. Repligen highly recommends running an integrity test after cassette installation. Refer to
TangenX™ Application Note AN1002 for instructions and information on cassette integrity
testing.
8. TangenX™ SIUS™ PD Cassette Equilibration
Cassettes must be equilibrated with an appropriate buffer (i.e., phosphate buffered saline) to ensure
the neutralization of the 0.2M sodium hydroxide storage agent in the membrane filter. Verify the
pH of the effluent from the cassette is neutralized to minimize any possible interaction with your
particular application. For most applications, further sanitization is not required.
9. Cassette System Cleaning
TangenX™ SIUS™ PD cassettes are intended for single use, therefore post-use cleaning and re-use is
not recommended nor supported by Repligen. Similarly, the Filter Plate Insert Kit is also designed
for single use, and like the TangenX™ SIUS™ PD cassette, post-use cleaning is not supported by
Repligen.
1.
Remove clamp top plate.