Qiagen QIAgility, Руководство пользователя, Страница: 128 / 334

QIAgility Software
5-66 QIAgility User Manual 11/2011
Mix count Defines the number of times the reagent
will be mixed at the start of the run. This
ensures that reagents are homogeneous.
Setting up reagents
1. Left-click on a plate or well with “Reagent” function in
the main software window. The “Reagent” right-hand
pane will appear.
2. Select “Use Reagents?”. A default reagent setup will
appear in the “Reagent Configuration” table
3. Enter information into the “Name”, “Def. Vol.”,
“Viscous?” and “Mix Count” columns. To do this, left-
click on the individual cells and enter the new
information. For the “Viscous?” column, check the box to
toggle between “Yes” and “No”. Then click on the “OK”
tab to update the table.
Note : If the default volume for a reagent is 0 µl, the
reagent can still be used in the master mix component
selection, but it will not be shown in the reaction
configuration.
4. When a reagent name is entered, the associated well will
appear orange on the plate on the software worktable. If
the reagent name remains blank, the associated well will
appear gray, and it will not be possible to use this
reagent in a master mix or reaction. If a reagent is used
in a master mix or reaction, it becomes more strongly
colored.
5.9.4 Master Mix
Master mixes can be prepared using the QIAgility, or a
premade master mix can be used. Master mixes can be
added to reaction wells. By default, master mix is pipetted
into a reaction well first, because it normally constitutes the
greatest volume of the reaction.
To prepare a master mix using the QIAgility, individual
reagents and their volume per reaction must be defined. The
order in which the reagents are added to the master mix can