ProteOn XPR36, Руководство пользователя

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ProteOn XPR36 | Experiments
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3. Load the 2 L buffer bottle in the right side of the buffer compartment.
Allow the buffer to come to room temperature before starting the
experiment.
4. Place the sample rack on the thermal platform in the autosampler
compartment.
Note: If you are changing buffers, you must prime the system
with the buffer you will use for the experiment.
Priming the System
Priming pumps the selected buffer through the instrument fluidics and through
the flow cell. It is a maintenance protocol function and is performed with a
maintenance chip inserted in the instrument. Priming flushes the system and
pumps buffer over the surface of the chip. Buffer flows through the instrument
fluidics at the maximum rate and through the flow cell at a rate compatible
with the flow cell. Priming fills the buffer system with the new buffer, eliminates
air bubbles in the system, and prevents signal drift resulting from the slow
change in refractive index during the transition between buffers. The system
can be primed only using ProteOn Manager software.
Prime the system under the following conditions:
• When starting up the instrument
• Before inserting a sensor chip when there is a maintenance chip
inserted and the instrument is in Maintenance Standby mode
Note: You cannot cancel the priming process after it starts.
To prime the system:
1. You can use two different buffers, one placed in buffer bottle A and one
in buffer bottle B. The active location is indicated by choosing either of
the illuminated buffer buttons (A or B) in the buffer compartment.
2. Insert a maintenance chip if one is not in place.
3. On the Instrument Maintenance screen, click Prime .
4. Follow all the prompts in the priming wizard. The priming process takes
up to 20 min, and the syringes go through seven cycles. During priming,
the status bar indicates that the instrument is in Maintenance state.
5. When priming finishes, click Finish. The Instrument Maintenance control
buttons are enabled, and the instrument state changes to Maintenance
Standby.