Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
·
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Part# TM338
Printed in USA.
Page 22
Revised 1/11
8750MA
QuantiFluor
Version XX xxxx
Indicates which channel is
active (A or B)
Indicates Fluorescence Reading
and activity of IDL
HOME screen
X.XX.X
UV
5-second warm up
3.B
Press
↑
or
↓
buttons to
adjust brightness of LCD
3.G
Press
CAL
button to
calibrate instrument
3.C
Press
A/B
button to switch
between optical channels
X.X
BLUE
GREEN (E6100 only)
X.X
3.F
Press
STD VAL
button to
view value of standard
X.X
BLUE
Cal Val: XX.X
<UP/DOWN>
BLUE
3.J
Press
DIAG
button to
view Diagnostics Screens
Logging Points
Left: XXX
Press
ENTER
button to toggle
between screens
RFU-BLK X.X
RFU-STD X.X
3.K
After calibration, insert the Solid Standard
(QuantiFluor ST only) and press the
READ
button.
Confirm that the value does not deviate from
previously recorded value by more than 5% or more.
If it does, recalibrate the instrument. If it does not,
record the reading.
continued on next page
Calib
<ENT> to start
BLUE
Press
ENTER
button to
move to next screen
Insert Blank
Then press <ENT>
Press
ENTER
button to
begin reading blank
Reading Blank
XX.X
Insert Cal Soln
then press <ENT>
Press
ENTER
button to
begin reading cal solution
Calib Complete
press <ENT>
Press
ENTER
button to
accept calibration
If
ENTER
button is not
pressed within five seconds,
the following screen will
appear:
Abort Cal?
<
↑
> Yes <
↓
> No
4.9
If the message
Standard<Blank, Recalibrate
appears, we recommend the standard
concentration be increased by 10X, or examine
the blank for high fluorescence background.
Note:
The RFU of the standard (RFU-Std) must be
3 times greater than the RFU of the blank
(RFU-Blk) to calibrate the instrument.
4.A
Press
DATA
button to
adjust data logging settings
Datalogger:
<DATA> to toggle
Press
DATA
button once
for Data Logging status
Press
DATA
button twice
to download data
Press
DATA
button three times
to erase data
Press
ENTER
button to toggle
between screens
Press
ESC
button to return
to the
HOME
screen
Press
ESC
button to return
to the
HOME
screen
Press
ESC
button to return
to the
HOME
screen
Press
ENTER
button five times
to begin download
If no data are logged, the
following screen will appear:
Data Logger has
no valid data!
Erase DATA
5X <ENT> to start
Press
ENTER
button five times
to begin erasing data
Erase DATA
All data erased
Status: Stop
<ENT> to toggle
Status: Logging
<ENT> to toggle
Download Data
5X <ENT> to start
8.
Appendix
8.A. QuantiFluor™ Fluorometer
Workflow Chart
