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Neurotechnology Research Systems

Stimulator 2.0

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5.2

Operational Flowchart

Start Stim 2

Software

Function

Stimulate

Rectangular

Pulses

First phase

amplitude

and width

Interphase

delay

Second phase

amplitude

and width

Number of

pulses

Rate

Trigger Mode

Load

Start

Monitor

Arbitrary

Waveform

Load File

Number of

pulses

Rate

Trigger Mode

Load

Start

Monitor

Function

Z Test

Automatically

loads

waveform

Start

Vmon Scaling

Monitor

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Содержание Stimulator 2.0

Страница 1: ...0 April 2011 Plexon Inc 6500 Greenville Ave Suite 700 Dallas Texas 75206 United States of America Tel 214 369 4957 Fax 214 369 1775 E mail info plexon com www plexon com Stimulator 2 0 April 2011 STI...

Страница 2: ...lexon Inc and it is proprietary and restricted solely to assist Plexon Inc customers Neither this document nor the contents may be disclosed copied revealed or used in whole or in part for any other p...

Страница 3: ...angular pulses 18 5 5 Verifying the output on an oscilloscope 19 5 6 Compliance voltage and stimulation failure 22 5 7 Automatic electrode discharge 24 5 8 Arbitrary Waveforms Complex Rectangular Puls...

Страница 4: ...Vmon scaling Z test mode 41 6 6 3 Z conversion 42 6 7 Function Stimulate Function Z test 42 6 7 1 Stimulate Mode 42 6 7 2 Impedance Test Mode 42 6 8 Trigger Mode 43 6 8 1 Software Trigger Mode 43 6 8...

Страница 5: ...Waveform Pattern Files 54 9 Stimulator Limitations 55 9 1 Maximum Compliance Voltage Varies with Stimulation Amplitude 55 9 2 Power on off Transients 55 9 3 Current Monitor with an Open Circuit 56 9 4...

Страница 6: ...may be used in an edge triggered or level triggered gated mode to initiate stimulation with microsecond latency Each channel also has a dedicated digital output to signal to other devices the precise...

Страница 7: ...n 14 20 A 10 A 7 Stimulation Cable Plexon 14 03 A 03 8 Test Board model electrodes Plexon 14 04 A 03 A International customers The stimulator power supply has an International Electrotechnical Commiss...

Страница 8: ...tion Read the entire Driver Installation section before proceeding with any of the steps 4 1 1 Browse to the folder Stimulator Driver on the USB flash drive and run the program CDM20802_Setup exe On s...

Страница 9: ...wse to the Stimulator Software folder on the USB flash drive 4 2 2 Double click the file Stim 2_Setup exe to begin the installation process On some computers you will get a warning 4 2 3 Click Yes or...

Страница 10: ...Plexon Inc The default install location for Windows XP is C Program Files Plexon Inc Click Next to continue 4 2 5 You will be asked to confirm that you would like to start the installation Click Next...

Страница 11: ...Neurotechnology Research Systems Plexon Inc 11 4 2 7 Once the progress bar starts moving the installation completes quickly Click Close to complete the software setup...

Страница 12: ...the power switch on the power supply is in the off position 4 3 2 Connect the AC power cord between the AC outlet and the power supply 4 3 3 Connect the DC power cord between the power supply and the...

Страница 13: ...een the stimulator and the test load board Be sure to line up the white orientation dots on both connectors 4 3 7 Connect BNC cables between the monitor channel outputs and an oscilloscope Monitoring...

Страница 14: ...and corner of the computer screen In Windows 7 the balloon changes from Installing device driver software to Your device is ready to use In Windows XP the balloon cycles through several different disp...

Страница 15: ...fixed rate More complicated rectangular waveforms and non rectangular arbitrary waveforms may be defined in and loaded from a simple text file Once defined the stimulation pattern is downloaded into...

Страница 16: ...ate Rectangular Pulses First phase amplitude and width Interphase delay Second phase amplitude and width Number of pulses Rate Trigger Mode Load Start Monitor Arbitrary Waveform Load File Number of pu...

Страница 17: ...efault configuration at any time by selecting Open from the File menu and then selecting the configuration file Factory_Default stm By default this file is installed in the directory C PlexonData Stim...

Страница 18: ...nter phase delay The first phase is 100 A for 50 s the inter phase is 0 A for 25 s and the second phase is 100 A for 50 s To make it easier to see on the oscilloscope we want to generate a continuousl...

Страница 19: ...rrent monitor is 2 5 mV A and by default the scale factor for the voltage monitor is 0 25 V V That means that if there is a 100 A current flowing into the electrode from the stimulator the current mon...

Страница 20: ...s law with respect to the access resistance of the electrode The subsequent rise in electrode voltage during the first phase of the pulse is due to charging of the electrode capacitance By definition...

Страница 21: ...ltage immediately drops by the same amount that it jumped at the beginning of the pulse VACCESS The current during the interphase period is zero With no current flowing into the electrode there is no...

Страница 22: ...col will depend on the amplitude of the current pulse the duration of the pulse the stimulator compliance limit and the properties of the electrode To see this effect first stop the ongoing stimulatio...

Страница 23: ...he monitor channel At this point the compliance limit has been reached and the output current as seen on the current monitor drops to zero The voltage however stays at its maximum value just to mainta...

Страница 24: ...returns to zero after the end of the pulse Ordinarily if the pulse delivery was not balanced and charge was left on the electrode then the voltage of the electrode would change after each pulse The o...

Страница 25: ...of rectangular pulses than can be defined using the front panel GUI controls The format of the arbitrary waveform text file is defined in section 6 5 To load an arbitrary waveform for channel 1 make...

Страница 26: ...search Systems Stimulator 2 0 26 Change the No of repetitions to be 2 and the Rate to 1000 Hz Then Load and Start the channel The output of the stimulator is Rate 1000 Hz 1 ms One waveform Repeated tw...

Страница 27: ...Z test mode that automatically generates a 100 nA 1 kHz sinusoid for impedance testing Locate the Function control towards the bottom of the GUI Press the Z test button to put the stimulator in imped...

Страница 28: ...peak amplitude of 7 60 mV Try changing the Vmon scaling from 4000 mVpp to 400 mVpp Now the output of the voltage monitor is a sinusoid with a peak to peak amplitude of 76 8 mV Continue to change the...

Страница 29: ...litude signal on the voltage monitor is 856 mVpp and the Vmon scaling is 40 mVpp so the impedance of the electrode is To obtain the most accurate impedance reading you should adjust the scale factor a...

Страница 30: ...ity of the electrode tissue interface over time This section shows some measurements obtained from a blunt cut 25 m diameter platinum iridium micro wire electrode in saline All of the measurements are...

Страница 31: ...increase in electrode voltage from 1 64 V to 2 64 V over the course of the 50 s pulse implies a capacitance of It is tempting to try and relate the impedance of the electrode at 1 kHz to the stimulat...

Страница 32: ...ger modes Rising and Level In the rising trigger mode stimulation begins when the digital input for the channel transitions from low 0V to high 5V In the level trigger mode stimulation also begins whe...

Страница 33: ...letion Compare that with the output in Level trigger mode To switch to level trigger mode you must first change channel 1 to edit mode Then switch to level trigger mode Finally load channel 1 again Th...

Страница 34: ...e user interface Pressing the Stop or Edit control for a particular channel causes the stimulation in progress on that channel to stop Pressing the Stop All Edit All or Abort controls or closing the u...

Страница 35: ...the channel is in edit mode Press the Edit button to put the channel in edit mode Pressing the edit button will cause any stimulation pattern that is in progress on the channel to stop 6 1 2 Load Onc...

Страница 36: ...orm output the remainder of that pulse or arbitrary waveform will play to completion This helps preserve charge balance in the stimulation output Any remaining pulses in the pattern will not be played...

Страница 37: ...d second phases The interphase delay may range from 5 s to 65535 s in steps of 1 s The current output is zero during the interphase delay 6 3 4 Second Phase Amplitude The second phase amplitude may ra...

Страница 38: ...r arbitrary waveform is repeated expressed in Hertz or the time between the start of one pulse or arbitrary waveform and the start of the next pulse or arbitrary waveform expressed in milliseconds Use...

Страница 39: ...o arbitrary waveform pattern file formats are supported One file format uses a fixed sampling rate and the other file format uses a variable sampling rate 6 5 1 Fixed Sampling Rate In the fixed sampli...

Страница 40: ...le code for additional amplitude duration pairs When the stimulation pattern is played back each current amplitude is played for the specific duration associated with that amplitude The variable sampl...

Страница 41: ...g with extremely low impedance electrodes Divide the voltage observed on the monitor output by the scale factor to determine the voltage at the electrode Note that if the Vmon scaling is set higher th...

Страница 42: ...nitor scale factor is handled automatically for you 6 7 Function Stimulate Function Z test 6 7 1 Stimulate Mode Select stimulate mode for rectangular pulse or arbitrary waveform stimulation 6 7 2 Impe...

Страница 43: ...ated 6 8 2 Rising Edge Trigger Mode In the Rising trigger mode stimulation begins when the digital input for the channel transitions from low 0V to high 5V The latency from digital input to stimulatio...

Страница 44: ...t during the stimulation pattern are ignored e g the pulse marked 3 6 9 Edit Load All When the Edit Load control is in Individual mode the mode of each channel can be configured using the individual E...

Страница 45: ...sing the Start All function First define and load the appropriate stimulation pattern for the channels you wish to stimulate and then initiate the multichannel stimulation pattern by pressing the Star...

Страница 46: ...eturn to the edit mode If the stimulator is in impedance test mode it will return to stimulate mode Consider a complete stimulation pattern that is composed of four pulses Pressing stop stop all edit...

Страница 47: ...e parameters are then automatically re loaded the next time you open the user interface Note that if any channels have been configured to use an arbitrary waveform pattern neither the fact that the ch...

Страница 48: ...ntentional electrode charging all electrodes are automatically discharged in between pulses and any time the channel is not stimulating Advanced users ONLY may disable the automatic discharge of the e...

Страница 49: ...al There is also a 17th digital output that goes high when ANY channel is playing back a stimulation waveform Digital output 17 with digital output mode set to low during inter pulse interval Digital...

Страница 50: ...s are TTL compatible 7 3 USB 2 0 The stimulator has a mini type B USB 2 0 receptacle for communications with the host computer The USB receptacle on the stimulator is connected to digital IO ground Th...

Страница 51: ...goes high when any channel is stimulating See section 6 15 Options Digital Output Mode for additional details 1 DO 1 3 DO 3 5 DO 5 7 DO 7 9 DO 9 11 DO 11 13 DO 13 15 DO 15 17 DO 17 19 Dig IO GND 2 DO...

Страница 52: ...the outer contact of the current monitor BNC connector is connected to analog IO ground The outer contact of the BNC connectors on many oscilloscopes is connected to the AC wall outlet ground Therefo...

Страница 53: ...to the impedance of the electrode See sections 5 9 Impedance measurement and 6 6 Vmon scaling and Z conversion for additional details Note that the outer contact of the voltage monitor BNC connector...

Страница 54: ...section 5 8 for an example of such a waveform created using the 3_pulse_burst_variable pat arbitrary waveform pattern file When using the GUI controls to define rectangular pulses the minimum interph...

Страница 55: ...resistor is used to measure the actual output current but reduces the voltage available to deliver to the electrode 9 2 Power on off Transients To avoid transients at the stimulator outputs caused by...

Страница 56: ...ient measured at the stimulator output is shown below For this reason disconnect the stimulator from the electrodes before turning the power off 9 3 Current Monitor with an Open Circuit Ideally when n...

Страница 57: ...n the cabling to the electrode the impedance test procedure will yield a finite but large impedance result With no cable connected to the stimulator this open circuit impedance will vary from channel...

Страница 58: ...rent 1 mA Stimulation mode Resolution 16 bits Output current resolution 30 nA Stimulation mode Compliance voltage 10 V 700 A see section 9 1 Temporal resolution 1 s Output rise time 1 25 s 100 A 10 k...

Страница 59: ...Neurotechnology Research Systems Plexon Inc 59...

Страница 60: ...Plexon Inc 6500 Greenville Ave Suite 700 Dallas Texas 75206 United States of America Tel 214 369 4957 Fax 214 369 1775 E mail info plexon com www plexon com Stimulator 2 0 April 2011...

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