PerkinElmer Wallac 1420, Руководство пользователя

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Tools menu
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Normalization confirmation page
This is the last page of the LANCE normalization wizard and appears after normalization
has been successfully completed. It shows you the measured normalization values and
requires you to confirm that you accept these values before they are stored in the database.
The values obtained are the blank measurement in the acceptor window, crosstalk, i.e. the
blank corrected contribution of the donor to the acceptor window signal as a proportion of
the donor signal, the maximum acceptor signal in the acceptor window and the maximum
donor signal in the donor window. These values are then used in a measuring sample to
obtain the actual acceptor value i.e. the blank and crosstalk corrected acceptor signal
normalized to the maximum donor signal (thus eliminating quenching effects). See the
Calculation methods in the instrument manual for more details.
Note: to avoid accidental clicking of the Finish button, it only becomes visible after five
seconds have elapsed since the page was opened. During the time the Finish button is
disabled the text of the button is ‘Wait t’, where t is the remaining waiting time and it is
updated every second. When you click
Finish , the new values replace the old values in the
database.