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Instrument Methods . 51

Concentration

The Concentration method makes simple concentration measurements on samples by

measuring the amount of light that has passed through a sample relative to a reference (this

can be air). The concentration is determined by measuring the absorbance at a specific

wavelength and then multiplying by a factor. If the factor is known in advance it can be

entered into the method. If the factor is not known, a standard of known concentration can

be measured and the factor then calculated by the instrument.

The procedure is as follows:

From the home page, press 1 to open the Standard Methods folder.
The Standard Methods screen is displayed.

Press 4 to open the Concentration method.
The Concentration – Method Settings screen is displayed.

Enter the

Wavelength

by using the keypad numbers or the left and right arrows, and

then press the down arrow.

Содержание Lambda XLS

Страница 1: ...LAMBDA XLS XLS User s Guide UV VIS SPECTROSCOPY...

Страница 2: ...lied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing p...

Страница 3: ...oftware 23 Methods and Favorites 27 Folder Options 27 Method Options 30 Lambda XLS XLS Instrument Methods 32 Utilities 33 Date and Time 34 Regional 34 Printer 35 Preferences 36 Contrast 37 About 37 Sa...

Страница 4: ...Appendices 85 Appendix 1 Specifications 86 Appendix 2 Customer Care Contacts 87 Index 89...

Страница 5: ...Introduction...

Страница 6: ...ribes the installation and operating procedures for your Lambda XLS XLS UV Vis spectrometer The manual contains the following sections Introduction Safety Information Installing your Instrument Using...

Страница 7: ...rs to the Lambda XLS or Lambda XLS instrument Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indica...

Страница 8: ...8 Lambda XLS XLS User s Guide...

Страница 9: ...Safety Information...

Страница 10: ...substances Europe EU directive 2002 95 EC RoHS Directive China Administration on the Control of Pollution Caused by Electronic Information Products No 39 This appliance is marked according to the Euro...

Страница 11: ...ter and allowed to dry Similarly the outer cover can be wiped over with an appropriate disinfectant with a wipe or soft cloth Any internal spills should be wiped up using absorbent material through th...

Страница 12: ...on of the equipment although the samples under test may require the use of such PPE and should be covered by a risk assessment made by the user or the appropriative Health and Safety representative In...

Страница 13: ...it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before turning the instrument on This will prevent calibration failure as a result of internal condensation The ins...

Страница 14: ...14 Lambda XLS XLS User s Guide...

Страница 15: ...Installing your Instrument...

Страница 16: ...on CD L7111025 Cuvette dust cover L7110231 Lambda Bio XLS Print Utility and Cable if applicable L7110270 UV macro cuvettes 8 pack N A Power cable 2 Examine the components for any signs of damage in sh...

Страница 17: ...d and the power cord that is appropriate for your region You must use the power cord and power supply supplied with the instrument NOTE If the instrument has just been unpacked or has been stored in a...

Страница 18: ...18 Lambda XLS XLS User s Guide...

Страница 19: ...Using your Instrument...

Страница 20: ...taking measurements and viewing your results Turns the instrument on or off Use the four arrows to navigate around the display and select the required setting from the active highlighted option The O...

Страница 21: ...key enters or confirms a selection moves to the Next screen or takes a measurement Alphanumeric keys Use these to enter Method Settings make Options menu selections and to write text descriptions whe...

Страница 22: ...to press OK to restart the calibration procedure However after three attempts you will still be able to view the default home page You should NOT use the instrument under these circumstances Instead m...

Страница 23: ...he method to take measurements Figure 4 Features of a typical Method Settings screen Navigation Move between settings using the up and down arrows Entering Method Settings Enter Method Settings by usi...

Страница 24: ...creen 1 Insert the Reference sample in the sample holder and press OA 100 T to take the reference measurement 2 Insert your first sample and press 3 Repeat Step 2 for each sample Results The results a...

Страница 25: ...to start taking measurements from 8 Save Method Allows you to save the Method Settings as a new method to the Methods or Favorites folders or if installed to an SD Memory Card You can save up to 9 me...

Страница 26: ...ve been appropriately set for your experiment before running your samples NOTE Setting the History method setting to On see Preferences on page 36 will cause the instrument to store its last settings...

Страница 27: ...also export backup methods to an SD card or import restore methods from an SD Memory Card Figure 7 Methods folder Folder Options Press to display the available Options in the Methods folder which you...

Страница 28: ...vailable Options 3 Press 2 to select Lock Folder 4 Select the Folder you wish to lock using the left and right arrows and then press the down arrow 5 Enter a Pass Code for the folder using the keypad...

Страница 29: ...select Restore Folder or Restore All Folders If you have renamed an instrument folder the folder name will be overwritten by those in the BACKUP directory of the SD Memory Card NOTE If you rename a fo...

Страница 30: ...e the Method 2 Lock Method Allows you to lock the method 3 Unlock Method Allows you to unlock the method Delete Method 1 Open the appropriate methods folder 2 Press to display the available Options 3...

Страница 31: ...2 Press to display the available Options 3 Press 3 to select Unlock Method 4 Select the method to be unlocked using the left and right arrows and then press the down arrow 5 Enter a Pass Code using th...

Страница 32: ...ur Lambda XLS XLS instrument are Single Wavelength Multi Wavelength Spectrum Concentration Standard Curve Kinetics Absorbance Ratio Figure 8 Lambda XLS home page NOTE If you have an SD Memory Card ins...

Страница 33: ...e Utilities screen is displayed Figure 9 The Utilities screen The Utilities are 1 Date and Time Set correct time and date 2 Regional Select preferred language and number format 3 Printer Printer outpu...

Страница 34: ...Month Year Hour and Minute 3 Press OK to store the settings to store the settings and return to the Utilities folder Seconds are zeroed when you press OK Regional 1 Press 2 to select Regional The Reg...

Страница 35: ...omputer via USB port SD Memory Card or Computer Bluetooth to a computer via Bluetooth The options available depend on what is installed in your instrument NOTE If SD Memory Card is selected data is sa...

Страница 36: ...reen layout of folders The options are either a Grid format or a List 4 Select the History mode using the left and right arrow keys If Off is selected the instrument will not remember your method sett...

Страница 37: ...2 Adjust the Brightness using the left and right arrows and then press the down arrow 3 Adjust the Contrast using the left and right arrows 4 Press OK to store the settings and return to the Utilities...

Страница 38: ...standard 10 40mm Lambda XLS or 10 mm Lambda XLS pathlength quartz glass or plastic cells The optical height is 15 mm and the minimum volume that can be used is approximately 70 l in a micro cell NOTE...

Страница 39: ...Instrument Methods...

Страница 40: ...n be air The procedure is as follows 1 From the home page press 1 to open the Standard Methods folder The Standard Methods screen is displayed 2 Press 1 to open the Single Wavelength method The Single...

Страница 41: ...ress This reference measurement will be used for all subsequent samples until repeated 7 Insert your first sample and press The result at the selected wavelength is displayed on screen Use the left an...

Страница 42: ...rayed out if no data are available 7 Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value 8 Save Method Use the left and right arrows to select the...

Страница 43: ...older The Standard Methods screen is displayed 2 Press 2 to open the Multi Wavelength method The Multi Wavelength Method Settings screen is displayed 3 Select the number of Wavelengths and then press...

Страница 44: ...ple and press This reference measurement will be used for all subsequent samples until repeated 8 Insert your first sample and press A spectrum covering the range of wavelengths is displayed with curs...

Страница 45: ...f no data are available 7 Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value 8 Save Method Use the left and right arrows to select the folder you...

Страница 46: ...ress 1 to open the Standard Methods folder The Standard Methods screen is displayed 2 Press 3 to open the Spectrum method The Spectrum Method Settings screen is displayed 3 Set the Start Wavelength by...

Страница 47: ...rt your first sample and press A spectrum and a table of the Absorbance Transmission values at each peak are displayed Use the left and right arrows to move the cursor along the graph When it reaches...

Страница 48: ...he peak to be detected Min Peak Width Minimum width of the peak as determined by the difference in wavelength between the higher of the two adjacent minima and the opposing intersection of that higher...

Страница 49: ...to discriminate between User Defined Peaks and Auto Detect peaks If the cursor is positioned over a user defined peak the legend User Defined Peak is displayed at the top of the scan The option then c...

Страница 50: ...s y axis limits Setting to On activates the start and finish points of the desired graph to user defined specific wavelengths and or absorbance values Press to cancel the selections press to accept th...

Страница 51: ...If the factor is known in advance it can be entered into the method If the factor is not known a standard of known concentration can be measured and the factor then calculated by the instrument The p...

Страница 52: ...he available range is from 0 001 to 9999 6 Enter the Units and then press the down arrow You can enter text up to 8 characters long using the keypad or press Options to display the Units screen and ac...

Страница 53: ...played 8 Insert the Reference sample and press This reference measurement will be used for all subsequent samples until repeated 9 If you selected Measure Standard insert your standard and press OK Th...

Страница 54: ...XLS XLS User s Guide 10 Press Run to run the standard The Factor is calculated and displayed 11 Insert your first sample and press The Concentration of the sample is displayed 12 Repeat Step 11 for ea...

Страница 55: ...by the cursor 4 Run Standard Returns to the Run Standard screen 7 Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value 8 Save Method Use the left an...

Страница 56: ...a zero concentration standard include this in the number of standards to be entered and enter 0 00 for the Concentration then use a reagent blank when required to enter the zero standard The procedur...

Страница 57: ...e selected so 98768 2 is displayed as 98768 even with 1 decimal point selected Press OK to save your options and return to the Method Settings screen You can still edit the units 6 Select the type of...

Страница 58: ...eypad numbers and the up and down arrows to move between the different standard boxes The available range is from 0 001 to 9999 11 Press Next The Standard Curve Calibration screen is displayed NOTE If...

Страница 59: ...Go to Step 15 The available range is from 0 001 to 9999 13 Insert your first standard and press If there are any replicates press Replicates to view them and then press to measure each replicate 14 R...

Страница 60: ...to return to the Standards screen 15 Press OK to accept the calibration The Standard Curve results screen is displayed 16 If you selected Manual insert the reference sample and then press This referen...

Страница 61: ...es the calibration graph on and off The cursors display the absorbance and corresponding concentration values of the last measured sample 7 Sample Number Adds a prefix to the sample number and resets...

Страница 62: ...ful information when an appropriate factor defined in the reagent kit protocol is applied Reaction rate and enzyme activity can be calculated if the factor used takes account of the absorbance differe...

Страница 63: ...down arrow The default value is 340 nm 4 Enter the Delay Time in seconds before measurements are taken and then press the down arrow This can be a maximum of 600 seconds 10 minutes 5 Enter the time in...

Страница 64: ...down arrow You can enter a text string up to 8 characters long To access a list of pre defined units on the Units screen press Options and then use the left right arrows g ml g l pmol l mg dl mmol l...

Страница 65: ...the bottom of the screen and absorbance data are plotted on the graph as the measurement proceed The table below the graph displays A0 the absorbance value at t0 start of calculation An the absorbance...

Страница 66: ...urrent cursor position The value is retained for subsequent samples 6 Show Slope Toggles the calculated slope line on and off NOTE If any data points enclosed by t0 and tn are beyond the range of the...

Страница 67: ...he home page press 1 to open the Standard Methods folder The Standard Methods screen is displayed 2 Press 7 to select the Absorbance Ratio method The Absorbance Ratio Wavelengths screen is displayed 3...

Страница 68: ...arrows and then press the down arrow The options are 5 mm or 10 mm 9 If the Dilution Factor is known enter a dilution factor by using the keypad numbers press the down arrow and then go to Step 13 The...

Страница 69: ...ypad numbers and then press the down arrow The available range is from 0 001 to 9999 15 Press OK to start measurements The Absorbance Ratio results screen is displayed 16 Insert the reference sample a...

Страница 70: ...the measured wavelengths which are marked by cursors 7 Sample Number Adds a prefix to the sample number and resets the incrementing number to the desired value 8 Save Method Use the left and right arr...

Страница 71: ...Accessories...

Страница 72: ...perkinelmer com for more information A range of cells and microcells are also available from PerkinElmer that are suitable for use with the Lambda XLS XLS instrument Part Number Accessory Consumable L...

Страница 73: ...rument Printer installation 1 Turn off the line power supply and disconnect the power cable 2 Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided 3 Tur...

Страница 74: ...g bosses and push down firmly 7 Reconnect the power cable Printer configuration 1 Switch the instrument on and then open the Utilities folder 2 Press 3 to open the Printer folder 3 Select Built in as...

Страница 75: ...nging the printer paper 1 Lift off the paper cover 2 Lock the platen and turn the knob to feed the paper 3 Feed in the paper Sometimes it helps if the platen lock is released 4 Replace the cover Plate...

Страница 76: ...wer cable from the instrument 2 Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided 3 Turn the instrument back over and lift the accessory cover vertic...

Страница 77: ...th module into the innermost slots in the base of the case 7 Lower the accessory cover vertically downwards onto the instrument engaging the PCB in the innermost slots in the accessory cover 8 Invert...

Страница 78: ...S User s Guide 10 Switch the instrument on and open the Utilities folder 11 Press 3 to open the Printer folder 12 Select Computer Bluetooth as the Printer option using the arrow keys 13 Press OK to sa...

Страница 79: ...ectly to any printer that is connected to that PC or saved The Report Utility can save data as an Enhanced Metafile emf graphics file a comma delimited csv data file a tab delimited txt data file or a...

Страница 80: ...80 Lambda XLS XLS User s Guide...

Страница 81: ...Maintenance...

Страница 82: ...ion methods except those specified here users should check with Customer Care that the proposed method will not damage the instrument Cleaning the instrument External Cleaning Switch off the instrumen...

Страница 83: ...Maintenance 83 2 Lower the sample holder out of the instrument Figure 10 Position of cell holder thumbscrews Thumbscrews...

Страница 84: ...ate is required before the instrument is shipped Decontamination certificates can be obtained from PerkinElmer The Troubleshooting Guide which will help you work through the returns process can be acc...

Страница 85: ...Appendices...

Страница 86: ...ccuracy 2 nm Wavelength reproducibility 1 nm Light sources Pulsed xenon lamp Detector 1024 element CCD array Absorbance range 0 300 to 2 500 A 0 to 199 T Photometric linearity 0 005 A or 1 of the read...

Страница 87: ...omer Care Customer Care USA 1 00 762 4000 In the USA 1 203 925 4602 Outside USA Customer Care EU 0800 40 858 Belgium 0805 111 333 France 0800 0 00 66 79 Germany 0800 90 66 42 Italy 800 099 164 Spain 0...

Страница 88: ...88 Lambda XLS XLS User s Guide...

Страница 89: ...e 22 Instrument Overview 20 K Keypad 20 Kinetics 62 L Language 34 M Maintenance 82 Methods 27 Absorbance Ratio 67 Concentration 51 Kinetics 62 Method folders 27 Method Options 30 Multi Wavelength 43 S...

Страница 90: ...andard Methods Absorbance Ratio 67 Concentration 51 Kinetics 62 Multi Wavelength 43 Single Wavelength 40 Spectrum 46 Standard Curve 56 Startup and Shutdown 22 Storage 84 U Using the Software 23 Utilit...

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