manualshive.com logo in svg

PerkinElmer LabChip GX Touch, Руководство пользователя

Поделиться
Скачать
PerkinElmer LabChip GX Touch Скачать руководство пользователя страница 69

Software Reference

69

P/N CLS151164 Rev. B

LabChip GX Touch/GXII Touch User Manual

PerkinElmer, Inc.

 

Change Password Window

Use the Change Password Window to change the password for the 
current user. This window is only available if the LabChip GxP 
option is installed with the LabChip GX Touch software. 

To open the Change Password Window, touch the 

User button

 on 

the 

Home Window

 and then touch the 

Change Password button

.

Figure 17. Change Password Window

The Change Password Window contains the following options:

Old Password

 - The current password for the current user.

New Password

 - The new password for the current user.

Confirm Password

 - The new password for the current user.

OK button

 - Saves the new password and closes the window.

Cancel button 

- Closes the window without saving changes to the 

password. 

Содержание LabChip GX Touch

Страница 1: ...User Manual LabChip GX Touch GXII Touch P N CLS151164 Rev B Publication Date October 12 2018...

Страница 2: ...d including but not limited to the implied warranties of merchantability or fitness for a particular purpose Some states or jurisdictions do not allow disclaimer of express or implied warranties in ce...

Страница 3: ...nElmer offers a full range of services to ensure your success From our original factory warranty through a comprehensive line of customer support plans PerkinElmer offers you Field Service Engineers a...

Страница 4: ...any interference received including interference that may cause undesired operation CE This device complies with applicable CE rules and requirements NOTE Changes or modifications to this equipment no...

Страница 5: ...nt an operating tip or maintenance suggestion may result in instrument damage if not followed REMARQUE nonc indiquant une pr caution prendre un conseil de fonctionnement ou une suggestion d entretien...

Страница 6: ...6 Fusible Alternating current IEC 60417 5032 Courant alternatif On power IEC 60417 5007 Marche alimentation Off power IEC 60417 5008 Arr t alimentation CE compliance mark Marque de conformit CE Batch...

Страница 7: ...Les tiquettes de l appareil sont cod es couleur Yellow Caution risk of danger Red Stop Blue Mandatory action Green Safe condition or information Jaune Attention danger potentiel Rouge Arr ter Bleu In...

Страница 8: ...9 Laser Safety on page 10 Electrical Safety on page 11 Mechanical Safety on page 13 Required Training Ensure that all personnel involved with the operation of the instrument have Received instruction...

Страница 9: ...ive equipment when handling chemicals e g safety glasses gloves or clothing For additional safety guidelines consult the MSDS Do not leave chemical containers open Use only with adequate ventilation i...

Страница 10: ...e red laser source 10 mW maximum continuous CW WARNING Use of controls or adjustments or performance of procedures other than those specified herein may result in hazardous radiation exposure NEVER re...

Страница 11: ...Touch GXII Touch instrument has internal lithium batteries Batteries should not be incinerated WARNING Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent...

Страница 12: ...0 volt input Power cord set fittings that is the appliance coupler and wall plug must bear the certification mark of the agency responsible for evaluation in the country where it will be used The appl...

Страница 13: ...ree axis robot that moves quickly and can be a pinch hazard Keep the front door of the instrument closed and keep hands away from the plate loading position when the robot is moving Keep hands away fr...

Страница 14: ...Plate Ladder Tube and Buffer Tube 31 Running an Assay 32 Defining the Run Parameters 33 Monitoring the Run 37 Editing the Analysis Settings during a Run 38 Stopping a Run before the Run is Complete 39...

Страница 15: ...ers 64 Central Data Repository CDR 65 CDR Security Suggestions 65 Software Reference 66 CDR Database Server Window 67 Change Assay Folder Window 68 Change Password Window 69 LabChip GX Touch Main Wind...

Страница 16: ...ectrodes 140 Cleaning the Microplate Carrier 140 Cleaning the Touch Screen 140 Cleaning the Optics Lens 140 Cleaning Test Chip C 141 Changing the Fuses 142 Running Software IQ 143 Running Operational...

Страница 17: ...res 160 Protein Clear HR Assay Failed Calibration 160 Protein Clear HR Assay Marginal Calibration 161 ProteinEXact Assay Failed Calibration 162 Troubleshooting Assay Problems 162 Software Problems 163...

Страница 18: ...Charge Variant CZE Chip and Reagent Kits are available to run specific assays on the LabChip GX Touch GXII Touch The Assay Kits include the reagents and consumables required to run the specific assay...

Страница 19: ...uding Specifications Safety Warnings Preparation Procedures Expected Results Troubleshooting LabChip Kit Essential Practices and Reordering Information is also located in the LabChip GX Touch GXII Tou...

Страница 20: ...arger than others The larger channels contain buffer During the chip preparation the smaller channels and some of the wells are filled with sieving gel and buffer Figure 1 Examples of DNA RNA Chip and...

Страница 21: ...ophoretically into the central channel As the fragments move down the central channel they separate by size and then pass the laser which excites the fluorescent dye bound to the molecule The software...

Страница 22: ...the chip depends on pH salt concentration and buffer additives The internal standards normalize these factors so that the software can use the ratio of the area of the curve of the standard to the unk...

Страница 23: ...directed in the LabChip GX Touch GXII Touch Assay User Guide 3 Purge the pressure lines see page 24 4 If necessary calibrate the optics see page 139 and run Diagnostics see page 152 5 Prepare the chip...

Страница 24: ...ouch Main Window opens The Navigation Bar displays on the left side of the LabChip GX Touch Main Window and the Home Window displays in the center Purging the Pressure Lines The LabChip GX Touch softw...

Страница 25: ...chip door on the front of the instrument unlatches 4 Lift the chip door up 5 Place the chip into the cutout with the sipper through the hole in the chip holder 6 Close the chip door and press down to...

Страница 26: ...er button and select the assay folder on the Change Assay Folder Window 5 Touch the Select Well Containing Standard Sample button to select the well that contains the VeriMAb sample 6 Select the desir...

Страница 27: ...rting the run 1 Insert the chip see page 25 2 Load the buffer tube see page 31 If running a test ladder load the ladder tube at the same time 3 Touch the Home button on the Navigation Bar 4 Touch the...

Страница 28: ...be placed on the plate before loading the plate into the instrument Figure 4 shows the size limits for the barcode label and the location on the microplate where the label should be placed The barcod...

Страница 29: ...mm Maximum Sip Height depends on the sample volume Sip Height of 4mm is probably safe To add a new plate 1 Touch the Tools button on the Navigation Bar 2 Touch the Plate Editor button to open the Plat...

Страница 30: ...plate punching holes in the tape The Punch test is acceptable if the punched hole is 0 5mm from the edge of the well Holes may not be perfectly centered Possible sources of errors Sipper splay Plate...

Страница 31: ...refully to properly prepare the plate 2 Fill the Ladder and Buffer tubes as instructed in the Assay Quick Guide 3 Touch the Home button on the Navigation Bar WARNING Pinch Hazard Keep hands away from...

Страница 32: ...ction 2 Define the parameters of the assay run see page 33 3 Monitor the run see page 37 4 If desired edit the analysis parameters during the run see page 38 5 If necessary stop the run before it comp...

Страница 33: ...ouch the Open button The settings specified in the run file display in the Select Wells Tab and Setup Run Tab To use the settings in the run file touch the Next button twice and skip to step 26 For mo...

Страница 34: ...he run 9 Touch the Next button The Setup Run Tab opens 10 If desired type the name of the operator in the Operator text box If the LabChip GxP option is installed the name of the current user is autom...

Страница 35: ...ings on page 46 for more information about selecting data to export 17 To automatically print the exported data to a PDF file after the run is complete select the Auto Print check box 18 To export all...

Страница 36: ...lect the Excluded Peaks File check box b Touch the Browse button next to Excluded Peaks File c Select the name of the GEP file that contains the excluded peaks and touch the Open button The path and n...

Страница 37: ...ed The warming step allows the heater plate in the chip holder to regulate chip temperature to 30 C Data collection begins after the priming and chip warming steps are completed 2 For ProteinEXact chi...

Страница 38: ...ry Editing the Analysis Settings during a Run Specific analysis settings can be adjusted in the LabChip GX Touch software while a plate is running If Auto Export is selected on the Setup Run Tab the A...

Страница 39: ...button on the Navigation Bar The run stops immediately If a well is in progress the data for that well is not saved A message box confirms that you want to stop the run in progress Figure 6 Stop Run...

Страница 40: ...data is saved in a new data file It is not added to the data file from the original run To continue a run 1 Touch the Run button on the Home Window 2 On the Select Wells Tab select the wells that were...

Страница 41: ...d 3 Remove the plate from the plate carrier 4 If necessary clean the plate carrier with DI water or 70 isopropanol solution 5 Remove the Buffer and Ladder tubes if necessary WARNING Pinch Hazard Keep...

Страница 42: ...sent washing protein chips on the LabChip GXII Touch immediately before the next use can often restore data quality 1 Prepare the chip and buffer tube as instructed in the Assay User Guide 2 Insert th...

Страница 43: ...ted to either CSV files or XML files CSV files can be imported into a spreadsheet program such as Microsoft Excel Raw Data can be exported to a CSV file an XML file or to an AIA file format a CDF file...

Страница 44: ...or example if peaks 3 5 and 7 are excluded when the data is exported into Microsoft Excel peaks 3 5 and 7 are not included Raw Data Either a CSV file ASCII text file an XML file or an AIA Format file...

Страница 45: ...TA Time Value Size 0 000 1 097 0 0 017 1 512 0 0 033 1 913 0 12 933 19 774 14 12 950 19 836 16 12 967 20 165 19 12 983 20 761 21 13 000 21 384 23 13 017 21 498 25 Gel Exports the gels in the selected...

Страница 46: ...select the Export as Single File check box If selected the gels for all wells are included in a single graphic file and the Raw Data for all wells is included in a single AIA CSV or XML file If Gel i...

Страница 47: ...desired properties into the exported list by selecting the properties and using the Left or Right arrow buttons to move the properties into the desired list 4 The changes take effect with the next fil...

Страница 48: ...open open the data file see page 53 2 Touch the Views button on the Navigation Bar 3 Touch the Select Wells button to open the Select Wells to View Tab 4 Touch the Export Plate button to export a dat...

Страница 49: ...on page 52 Viewing Data in the Gel Tab on page 52 Zoom In and Zoom Out on page 52 Viewing a Data File after a Run is Complete on page 53 NOTE After a run is complete use the LabChip GX Reviewer softw...

Страница 50: ...s on zooming graph and gel views Viewing Data in the Egram Gel Tab The Egram Gel Tab displays a graph at the top of the tab and the gels of all the well data at the bottom of the window The graph at t...

Страница 51: ...in the same graph Figure 10 Graph with Multiple wells To remove a specific sample from the graph touch the sample that you want to remove in the Select Wells to View Tab or Egram Gel Tab To display o...

Страница 52: ...luding marker peaks and system peaks To change the graph view options select the Graph Settings check box and select the desired options in the Graph Settings Viewing Data in the Gel Tab To compare th...

Страница 53: ...tab or the EGram tab double tap the graph to zoom out to the previous zoom level Viewing a Data File after a Run is Complete To view a data file after a run is complete 1 Touch the Views button on the...

Страница 54: ...ct the desired data to export See Selecting the Auto Export Settings on page 46 for details 2 Touch the Home button and then touch the Run button 3 Select the desired assay plate and sampling options...

Страница 55: ...le Names files are also used to import sample names into a data file as the data file is created This section includes general instructions for using Sample Names files in the LabChip GX Touch softwar...

Страница 56: ...Navigation Bar 2 Touch the Sample Names button to open the Sample Names Tab 3 Touch the Blank 96 Wells or Blank 384 Wells button to open a blank Sample Names file with 96 or 384 rows The default Sampl...

Страница 57: ...Reviewer User Manual if the file is created with Excel or a LIMS Sample Names files that are created in LabChip GX Touch software see page 56 always contain all 96 or 384 wells on the plate Existing...

Страница 58: ...reated by a LIMS or can be created in Microsoft Excel and are saved as a csv file To select the wells using a Sample Names file 1 Create the Sample Names file with one row for each well to be sampled...

Страница 59: ...the corresponding Sample Names file To use the plate barcode to specify the Sample Names file 1 Decide on a location and file name format for the Sample Names files For example the Sample Names files...

Страница 60: ...e default Sample Names assigned during a run can be edited after the run starts but before the sample well read is complete After the well has been read the data is saved in the data file and the samp...

Страница 61: ...to a remote CDR server is lost after a run is started the data is saved to a local folder and copied to the CDR when the network connection is restored Organizing Retrieving and Backing Up Data Files...

Страница 62: ...atory processes and procedures must comply with 21 CFR Part 11 regulations The LabChip GxP option ensures that assays output data analysis settings event data and backup data files are not available f...

Страница 63: ...er After the software is locked only the logged in user or a LabChip GX Touch Administrator can unlock the software To lock the LabChip GX Touch software 1 On the Home Window touch the User button in...

Страница 64: ...he LabChip GX Touch software and the previous user is logged out User Accounts Access to the LabChip GX Touch software is controlled by user accounts when the LabChip GxP option is installed Each user...

Страница 65: ...ables you to create new folders rename existing folder and delete empty folders CDR Security Suggestions To ensure proper security of data files the LabChip GX Touch Administrator should 1 Change the...

Страница 66: ...te Window on page 76 Prime Window on page 77 Run Window on page 78 Select Wells Tab on page 79 Setup Run Tab on page 81 Start Run Tab on page 84 Status Window on page 86 Tools Window on page 89 Export...

Страница 67: ...he CDR Database Server Window contains the following options Server Name IP Address The server name or IP address of the computer where the CDR is installed Apply button Connects to the CDR on the spe...

Страница 68: ...ing a plate To open the Change Assay Folder window touch the Change Assay Folder button on the Prime Window or on the Select Wells Tab Figure 16 Change Assay Folder Window The Change Assay Folder wind...

Страница 69: ...e To open the Change Password Window touch the User button on the Home Window and then touch the Change Password button Figure 17 Change Password Window The Change Password Window contains the followi...

Страница 70: ...h GXII Touch User Manual PerkinElmer Inc LabChip GX Touch Main Window Use the LabChip GX Touch main window to access the Navigation Bar and Home Window The LabChip GX Touch Main Window opens when the...

Страница 71: ...run is started or the software is closed data from a completed run is no longer available in the LabChip GX Touch software Use the LabChip GX Reviewer software to view or analyze data from previous r...

Страница 72: ...s of the instrument and user information also display If the LabChip GxP option is installed the Home Window displays the CDR Server status and provides access to the Login window To open the Home win...

Страница 73: ...debris out of the pressure lines to improve instrument performance Unload Chip Unlatches the front door to access the chip Manually close the door to load the chip Instrument Status Displays the curr...

Страница 74: ...y the user Server is Not available The software is not connected to the server Server not mapped The software has not been mapped to a CDR server User button Only displays if the LabChip GxP option is...

Страница 75: ...pecify additional password requirements NOTE The Switch User Change Password and Lock buttons are only available if a user is already logged into the LabChip GX Touch software Switch User button Enabl...

Страница 76: ...ndow contains the following options Change Assay Folder button Touch to select the folder that contains the desired assays Select Assay drop down list Touch to select the assay to run A1 button Touch...

Страница 77: ...r tube and the chip before opening the Prime window To open the Prime window touch the Prime button on the Home Window Figure 22 Prime Window The Prime window contains the following options and button...

Страница 78: ...ch GXII Touch User Manual PerkinElmer Inc Run Window Use th Run Window to define the assay run parameters To open the Run Window touch the Run button on the Home Window The Run Window contains the fol...

Страница 79: ...ect Wells tab contains the following options and buttons Change Assay Folder button Touch to select the folder that contains the desired assays Select Assay drop down list Select the type of assay to...

Страница 80: ...the wells in each column are sampled from top to bottom before proceeding to the next column A1 B1 C1 A2 B2 C2 Ladders are sipped every 12 wells so some columns will include a ladder in the column If...

Страница 81: ...etup Run tab on top of the Run Window Figure 24 Setup Run Tab The Setup Run tab contains the following options Operator text box Specifies the name of the operator running the assay The Operator Name...

Страница 82: ...ted If the LabChip GxP option is installed and the network connection is interrupted data files are archived in a local folder and copied to the CDR when the network connection is restored Copy To che...

Страница 83: ...selected the time that the run was started is included in the data file name The time format is HH MM SS where HH is the hour 00 to 24 MM is the minutes and SS is the seconds Sample Names File If sele...

Страница 84: ...r software to create the Excluded Peak file See page 170 for more information about Excluded Peak files The Setup Run tab contains the following buttons Back Opens the Select Wells Tab to change the s...

Страница 85: ...date is stored on the RF ID tag on the chip Chip Life Displays the number of samples until the chip expires Chip Life depends on the chip type Run Parameters Displays the settings selected on the Sele...

Страница 86: ...tatus of the chip The LabChip GX Touch automatically detects the chip type from an embedded RF ID tag in each chip Type Displays the type of chip in the instrument or the assay type selected for the c...

Страница 87: ...plays the status of the last operation Step Time Left The time remaining in the current step in the run Run Time Left The time remaining until the assay is complete Barcode Displays the barcode number...

Страница 88: ...igure 27 Calibration Steps for ProteinEXact NOTE If the ProteinEXact calibration steps do not display restart the software and prime the chip Realtime Electropherogram of Well Displays the electropher...

Страница 89: ...ettings view or add plates run the software IQ run Diagnostics and calibrate the optics To open the Tools window touch the Tools button on the Navigation Bar Figure 28 Tools Window The Tools window co...

Страница 90: ...the Tools button on the Navigation Bar and then touch the Export Setup button Peak Tables and Well Tables are exported to either CSV files or XML files CSV files can be imported into a spreadsheet pr...

Страница 91: ...etes Peak Table If selected the data in the Peak Table is exported to a CSV or XML file Well Table If selected the data in the Well Table is exported to a CSV or XML file Raw Data If selected the raw...

Страница 92: ...file in the Chromatography Data Interchange Format formerly AIA Include Size Data and Export as Single File are not available If the AIA Format check box is NOT selected the raw data is exported to a...

Страница 93: ...ilable for export do not display until after the run is started Figure 30 Peak Properties Tab Not exported list box Lists the columns in the Peak Table that are not included in the Exported Peak Table...

Страница 94: ...ilable for export do not display until after the run is started Figure 31 Well Properties Tab Not exported list box Lists the columns in the Well Table that are not included in the exported Well Table...

Страница 95: ...the Navigation Bar and then touch the Diagnostics button Figure 32 Diagnostics Tab The left side of the tab displays the tests to run on the instrument Expand each section to view all of the tests in...

Страница 96: ...eport of test limits After the report is generated the Limits Report Generation section at the bottom of the right side of the tab displays the date and time when the limit report was generated the na...

Страница 97: ...ntains the following options and buttons Reference Contrast The Contrast value from Test Chip C that will be compared to the scan results Contrast Instrument Gain Correction The current correction fac...

Страница 98: ...Abort Stops the scan or warm currently in progress Apply button Saves the New Correction Factor to the instrument s non volatile flash memory Plate Editor Tab Use the Plate Editor tab to view add or...

Страница 99: ...le when a plate is selected on the Custom Plates tab Add Plate button Opens the Add Plate Window to enter the information for a new plate Only available when the Custom Plates tab is selected Modify S...

Страница 100: ...Y Margin The distance from the outer edge of the plate to the center of well A1 in the Y direction Plate Height The distance from the bottom of the plate to the top of the plate Well Depth The distanc...

Страница 101: ...Structure Verifies that all original software folders exist If all expected folders exist displays Passed If any folders are not found displays Failed Files Verifies that all files that were installe...

Страница 102: ...after the IQ has been completed The detailed results are only available until the software is closed or another IQ test is run Figure 37 Detailed Result Tab The Detailed Result tab contains the follow...

Страница 103: ...h the Error or Warning button to display the event in the EventLog Tab Error messages can result from hardware or software problems Most are the result of peaks not being located by the analysis algor...

Страница 104: ...ic lock option is set in the LabChip GX Reviewer software The Unlock Application window only displays if the LabChip GxP option is installed Figure 39 Unlock Application Window The Unlock Application...

Страница 105: ...me of the Analysis Settings can be changed in the GX Touch software To control all of the Analysis Settings open the GXD file in LabChip Reviewer To open the Views window touch the Views button on the...

Страница 106: ...Plate Cycles is greater than one displays the cycle of the selected well Does not display if Plate Cycles equals one Perform Sample Analysis check box If selected the analyzed data displays in the Eg...

Страница 107: ...wells to view in the Egram Gel Tab EGram Tab and Gel Tab Touch a well to select it The status of the well is indicated by the color displayed in the plate diagram as shown below Well Color Blue Wells...

Страница 108: ...b If Overlay is selected the foremost graph is the last well selected To bring a graph to the front touch the gel at the bottom of the tab The Overlay legend displays the well number and the sample na...

Страница 109: ...tion Bar and then touch the EGram button Figure 42 EGram Tab Touch and drag over a region on the electropherogram to zoom in Touch the Unzoom button or the Unzoom All button to zoom out Graph data can...

Страница 110: ...d displays graph view options for the EGram tab and the graph in the EGram Gel tab See Graph Settings for a description of each option Data File Name Well Name The data file name and well name display...

Страница 111: ...ons Specifies the orientation of the text for the upper or lower annotation horizontal vertical up or vertical down Font button Opens the Font window to choose the font style and size of the text for...

Страница 112: ...y the annotations If not selected all peaks display the annotations Show Smears check box If selected displays smears as a colored line on the trace and displays the smear baseline This option only di...

Страница 113: ...ne Wells selected in the Gel view are also selected in the Select Wells to View Tab Egram Gel Tab and Gel Tab To open the Gel Tab touch the Views button on the Navigation Bar and then touch the Gel bu...

Страница 114: ...d location Double tap the Gel tab to zoom out to the previous zoom level To change the Lane Width Gel Contrast Range or Gel Color see Gel Settings The Gel tab contains the following options Overlay ch...

Страница 115: ...e slider to manually set the width Contrast Range Sets the minimum and maximum Gel Band Contrast for the bands in each well Use the sliders or type the value in the text box to change the min and max...

Страница 116: ...family selected for the chip selected when the chip is inserted in the instrument Assay Name The name of the assay that was run on the plate Software Version The version of LabChip GX Touch software...

Страница 117: ...e plate to the top of the plate Run Start Date Time The date and time that the run was started Number of Cycles The number of times to repeat the assay on the plate Sip Order Column wise or row wise a...

Страница 118: ...ned Calibration Ladder EP Mobility The mobility of the 120 kDa ladder peak during calibration Calibration Final DSR Applied The Final DSR applied during instrument calibration Final Percent Purity Obt...

Страница 119: ...ng the current session Past Events Tab Displays all events from all previous sessions If more than 10 000 events occurred scrolling controls for Page Up earlier events and Page Down later events displ...

Страница 120: ...e Names tab contains the following options Row Order option button If selected the rows in the table are in order by row letter and then by column number A1 A2 A3 etc Column Order option button If sel...

Страница 121: ...es the current sample names and user comments to a Sample Names file CSV with the specified file name The sample names are saved in the file in the same order as displayed in the table Blank 96 Wells...

Страница 122: ...elected on the Setup Run Tab Analysis Settings cannot be saved after a run is complete To open the Analysis Settings tab touch the Views button on the Navigation Bar and then touch the Analysis Settin...

Страница 123: ...ion stops The Gel and Graph views will not plot data beyond this time Baseline start 1sec before Specifies the time in seconds after the start of the run when the first peak can appear The Baseline st...

Страница 124: ...w to view the chip status and to start washing the chip See the Assay User Guide for chip prep instructions To open the Wash window touch the Wash button on the Home Window Figure 50 Wash Window The W...

Страница 125: ...in this section are the same for the GX and GXII Touch instruments The LabChip GX Touch runs DNA and RNA assays The LabChip GXII Touch runs DNA RNA Protein Glycan and Protein Charge Variant assays Th...

Страница 126: ...B LabChip GX Touch GXII Touch User Manual PerkinElmer Inc Front View Figure 51 shows the front view of the LabChip GX Touch GXII Touch and identifies the parts on the front of the instrument Figure 51...

Страница 127: ...f the computer has locked up Status Indicator Lights Indicate the status of the instrument Not Lit Power is off Solid Green Power is on GX Touch software is active but instrument is idle Flashing Gree...

Страница 128: ...onnect the LabChip GX Touch GXII Touch instrument to the power supply Figure 52 Rear Connector The following connectors are located on the back of the instrument AC Power Cable Plug the power cord int...

Страница 129: ...Touch instrument and to connect the instrument to a network Figure 53 Side Connectors The following connectors are located on the right side of the instrument Network USB Ports External VGA Monitor P...

Страница 130: ...can be acquired and stored at rates from 20 to 120 Hz Optical Train The fluorescence excitation fluorescence detection and autofocus optical trains contain several lenses and high efficiency interfere...

Страница 131: ...II Touch User Manual PerkinElmer Inc DNA RNA and Protein Chips The figures below show the format and parts of the microfluidic chips used in the LabChip GX Touch GXII Touch instruments to perform DNA...

Страница 132: ...ure to prime the chip with separation gel and provides vacuum to the chip wells to pull samples from the microplate into the chip The Chip Interface also contains a heating element to maintain a const...

Страница 133: ...n in either constant voltage or constant current mode Lid Holds the electrodes and o rings to supply voltage and pressure to the chip Chip Interface Holds the chip The sipper on the chip extends throu...

Страница 134: ...ves the microplate to be accessed by the LabChip Figure 58 Microplate Carrier Parts Buffer Tube Ladder Tube Sample Well A1 Locator Spring Locator Spring Microplate Carrier Holds the microplate Micropl...

Страница 135: ...well microplate Ventilation Cooling 3 77 mm minimum space required around instrument for proper air flow Operating Temperature 65 to 78 F 18 to 26 C Operating Humidity 20 to 70 relative humidity nonc...

Страница 136: ...tion Light Source Red laser diode Barcode Reader Temperature Range Ambient 5 C to 40 C no active cooling Accuracy 0 5 C Thermal limit switch 65 C 70 C Detection Wavelength Bandpass 670 725 nm Data rat...

Страница 137: ...unning Software IQ on page 143 Running Operational Qualification OQ on page 144 The LabChip GX Touch GXII Assay User Guides and Quick Guides contain LabChip Kit Essential Practices and instructions fo...

Страница 138: ...otion If the O rings stick to the chip or a pressure leak is detected perform the more extensive monthly cleaning procedure below NOTE Clean O rings and electrodes with DI water only Using 70 isopropa...

Страница 139: ...nd Test Chip warming step starts Warm up takes 10 minutes 6 Wait while the scan is performed The scan takes 5 minutes When the scan is complete the correction factor is calculated and displays in the...

Страница 140: ...as necessary Glass cleaner isopropyl alcohol or ethanol can be used on the touch screen When cleaning spray cleaner onto a cloth and wipe the screen with the cloth WARNING Do not spray cleaner direct...

Страница 141: ...from oil free compressed and filtered house air or compressed air cans Do not shake can before use Hold can at a distance from the chip to prevent condensation 3 To remove dyes or reagents from the R...

Страница 142: ...rd Disconnect the power cord before changing the fuses For continued fire protection and correct functioning of the unit replace fuses only with the exact part number to prevent fire To replace the fu...

Страница 143: ...nstallation Qualification can be used to check software installation qualification after routine computer maintenance such as disk cleanup after installing antivirus software or after installing Micro...

Страница 144: ...fication whenever required by your laboratory procedures The Diagnostics tab displays the tests that are performed on the left side of the window The test results are displayed on the right side of th...

Страница 145: ...Lock Ups on page 164 Cannot Connect to Remote CDR Server on page 164 Zipping the Log Files on page 165 Searching for Events in the EventLog Tab Events and errors display in the EventLog Tab Events and...

Страница 146: ...of the event or error Event Code The event error ID number used by PerkinElmer to troubleshoot problems Description A detailed description of the event or error that occurred Viewing Past Events in t...

Страница 147: ...orted Touch the Error or Warning button to display the event in the EventLog Tab The error or warning description describes the problem and either contains information on how to resolve the problem or...

Страница 148: ...6 Restart the LabChip GX Touch software Do not enable hibernation on the LabChip GX Touch GXII Touch instrument If the problem is not resolved contact PerkinElmer Technical Support see page 3 2 Comput...

Страница 149: ...es Recommended Actions 1 Robot failure Check for an object blocking the robot motion and remove the blockage Restart the instrument If the problem is not resolved contact PerkinElmer Technical Support...

Страница 150: ...chip as necessary Possible Causes Recommended Actions The last assay that the chip was primed for does not match the assay that is going to run 1 Touch the No button remove the chip refresh with appr...

Страница 151: ...ns Chip heater failed Restart the instrument If problem persists contact PerkinElmer Technical Support see page 3 Possible Causes Recommended Actions 1 Network cable unplugged or not connected to the...

Страница 152: ...anges have been made to the software The IQ can be run whenever required by your laboratory procedures The Installation Qualification can be used to check software installation qualification after rou...

Страница 153: ...o bottom prompting you to load the correct chips or plates as required for each test Follow the prompts As each test is completed the results display on the right side of the window Supplies Required...

Страница 154: ...Tag Test on page 156 Pressure Leak Test on page 156 Chip Temperature Test on page 156 Current Leakage Test on page 157 Laser Test on page 157 Plate Handler Test on page 157 Optics Test on page 158 HV...

Страница 155: ...temperature is not in valid range RH Temperature RH temperature is not in valid range Description Potential Cause of Failure Marker Pressure Sensor Test Pressure sensor out of calibration Contact Per...

Страница 156: ...Technical Support see page 3 Description Potential Cause of Failure Marker Pressure O Ring Leak test Pressure leak at O rings Clean or replace the O rings see page 138 and clean the chip interface Pro...

Страница 157: ...ription Potential Cause of Failure Leakage Check Channel 0 1 2 3 5 6 and 7 Source current too high or sum of sink currents too high Clean or replace the O rings see page 138 and clean the chip interfa...

Страница 158: ...indicate laser burned out or detector failure Contact PerkinElmer Technical Support see page 3 Description Potential Cause of Failure Optics Calibration Test Signal out of range indicates laser optic...

Страница 159: ...de Test Use any Chip Test Plate required Description Potential Cause of Failure Voltage Calibration Channel 0 1 2 3 5 6 and 7 Voltage out of calibration or electrode not connected to power supply Cont...

Страница 160: ...ssay may display pop up windows indicating failed calibration outcomes Failed calibration indicates the assay is not able to collect data Possible Causes Recommended Actions 1 Poor ladder quality Clea...

Страница 161: ...ram trace could not be eliminated Touch the Yes button to run the chip despite the issue displayed on the pop up window OR Touch the No button to fix the issues displayed on the pop up window To fix t...

Страница 162: ...PerkinElmer web site at www perkinelmer com If the problem is not resolved by following the suggestions in the Assay User Guide Contact PerkinElmer Technical Support see page 3 Possible Causes Recomm...

Страница 163: ...file name Read only files can be edited and saved with a new name or in a new location with the same name but cannot be saved over the original file Hard drive is full Verify there is sufficient free...

Страница 164: ...te keys on the keyboard simultaneously Perform a hard reboot by turning off the computer press and hold the Power button and then restarting it 5 Contact PerkinElmer Technical Support see page 3 Canno...

Страница 165: ...echnical Support To zip the LabChip GX Touch diagnostics logs 1 In the LabChip GX Touch software touch the Information i button in the lower right corner of any window to open the InfoView window 2 To...

Страница 166: ...Data Files Data Points DNA Assay Analysis Electrokinetic Forces Electroosmotic Flow Electrophoresis End Point End Time Excluded Peak File Expected Peak File GXD Files Hardware Diagnostics Inflection T...

Страница 167: ...he LabChip GX Reviewer software Baseline A baseline is established just after the start time setting After the overall baseline is established a local baseline is calculated for each peak to compensat...

Страница 168: ...y saved to the plate data file gxd As the data from each single well is received it is saved to the data file The name of the data file is specified in the Setup Run Tab If a run is stopped before the...

Страница 169: ...aks The molarity of each sample peak is calculated using the sample concentration the DNA fragment size in base pairs attributed to the peak and the known molecular weight of the DNA base pair Electro...

Страница 170: ...the data file is created The Excluded Peak file contains the size or time of the excluded peaks the color used to identify each excluded peak and a name for each excluded peak Excluded Peak Files are...

Страница 171: ...reviewing LabChip GX Touch data in the LabChip GX Reviewer software Lab on a Chip The generic term for a microfluidic product signifying a chemical process or material movement taking place on a micr...

Страница 172: ...do not use ladders or markers Log Files The LabChip GX Touch software log file displays in the EventLog Tab The log file maintains a running record of all events that occur with the instrument while...

Страница 173: ...ivers different absolute fluorescence on different instruments making it difficult to compare the e grams of similar samples run on different instruments To provide a more consistent absolute fluoresc...

Страница 174: ...and end points of adjacent peaks in the cluster are averaged to the same point so that no gaps exist between peaks Peak Identification From the smoothed data peaks are identified using a hill climbing...

Страница 175: ...t ladder Then each sample is shifted in time so the sample s lower marker aligns exactly with the primary ladder lower marker After alignment the size of the protein producing each peak is calculated...

Страница 176: ...s appear earlier in electropherogram The software can be used to track expected variants based on migration time and to determine the relative amount of each variant based on peak area Protein Charge...

Страница 177: ...reas and rRNA peak heights and some relevant ratios are exported in the Well Table A combination of the quantities is used to assess the quality of the RNA sample Analysis settings can only be changed...

Страница 178: ...nty Period Customer must notify PerkinElmer of any Warranty claim prior to expiration of the Warranty Period Notwithstanding anything to the contrary contained herein the Warranty Period for data proc...

Страница 179: ...M EXTENT PERMISSIBLE UNDER APPLICABLE LAW PERKINELMER EXPRESSLY DISCLAIMS ALL OTHER WARRANTIES OF ANY KIND EXPRESS IMPLIED OR STATUTORY INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTA...

Страница 180: ...ip of fifty percent 50 or more of the shares of stock entitled to vote for the election of directors in the case of a corporation or fifty percent 50 or more of the equity interest in the case of any...

Страница 181: ...the development of a competing software product or service k install or use the Software on more than one computer simultaneously without the purchase of additional licenses and or the express writte...

Страница 182: ...tware is that for a period of ninety 90 days from the date of receipt the Software will substantially contain the functionality described in the published specifications and when properly installed on...

Страница 183: ...is entitled to receive its costs and expenses including reasonable attorneys fees incurred in that action in addition to any other relief it may receive b If any term or provision of this Agreement is...

Страница 184: ...59 Supported Barcode Types 135 Baseline 167 Baseline Start 122 Bubble 168 Buffer tube 134 inserting 31 removing 41 C Calibrating the instrument 97 to run Protein Clear HR Assays 26 Calibrating the op...

Страница 185: ...Flow 169 Electropherogram Graph settings 111 Electrophoresis 20 169 End Point 170 End Time 122 170 Error Messages 147 Chip primed for different assay warning 150 Chip Temperature Warning 151 Current...

Страница 186: ...ion 125 preparing to run samples with 23 safety 8 Usage statement 18 Introduction 18 IQ running 143 152 L LabChip GX Touch instrument description 125 Main Window 70 software description 66 Usage state...

Страница 187: ...apacity 135 custom 98 height 29 inserting 31 orientation 135 predefined 98 removing 41 Plate Carrier Motion Blocked Error 148 Plate Editor Tab 98 Plate Handler Test 157 Pollution Degree 135 Position b...

Страница 188: ...Analysis 106 Sample Names file 55 177 creating 56 using barcode 59 using to select wells 57 Sample Names tab 120 Sample Names editing 60 Save run parameters to file 54 Saving data files 61 Searching...

Страница 189: ...ome Timeout 148 HV Check Failed 150 Maximum samples exceeded 150 Move Timeout 149 Plate Carrier Motion Blocked 148 Pressure Leak Detected 149 U USB Ports 129 User Comments 120 V Ventilation 135 Verify...

Страница 190: ...PerkinElmer Health Sciences Inc 68 Elm Street Hopkinton Massachusetts 01748 TEL 203 925 4602 FAX 203 944 4904 http www perkinelmer com...

Отзывы:

Нет отзывов

Бренды по названию

Популярные бренды

Загрузить еще бренды