Partec CyFlow space, Руководство по эксплуатации прибора

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Operating Basics
Rev. 007 Date 2010-01-13 CyFlow
®
space Instrument Operating Manual
16/26
Sample Speed
Speed
Note
For a high accuracy
measurement, a low
speed
smaller than
1.0µl/s is advisable.
Typical effect of
change
EAT cells, Speed
= 3µl/s
EAT cells, Speed
= 20µl/s
Adjustment
By means of the speed value, the sample speed (in
terms of µl/s) is set.
The speed value can be increased (decreased) by
clicking into the corresponding field in the instrument
settings box and using the Right (Left) buttons (see
Fig. 4).
The count rate increases with elevated speed
values. If the speed is increased too much, peaks in
the histograms may become wider as a result of
decreasing accuracy.
If the speed is too low, particle sedimentation effects
can influence a counting result.
Typical Speed Values
a) High accuracy measuremens
(e.g. DNA with < 1.5% CV):
Speed = 0.5 µl/s.
b) Fast measurements and absolute counting:
Speed = 4.0 - 10.0 µl/s
Fig. 6: Sample Transporting System of the
CyFlow
®
space
.