Partec CyFlow space, Руководство по эксплуатации прибора

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Operating Basics
Rev. 007 Date 2010-01-13 CyFlow
®
space Instrument Operating Manual
13/26
The Parameter Setup Dialog Box: Pulse Height, Area and Width
Opening the
Parameter Setup
Before starting an acquisition, assure the
parameters of interest are selected and set up
according to your analysis.
Click the "Parameters..." button in the
Instrument Settings box.
The Parameter Setup Dialog Box appears.
Fig. 5: The Parameter Setup Dialog Box
Note
Parameter names
cannot be changed
after an acquisition.
Parameter names
Pulse Property
Selections
Low Pass
2 Wavelengths
Signal Delay
Check if the parameter names are according to your
optical setup. Typical names are shown in the
example above. The parameter names are
displayed on the histogram axis together with a
parameter label. To change parameter names, click
into the fields and enter the names by using the
keyboard.
For each optical channel, you may analyse three
different pulse properties: 1. The pulse height, 2. the
pulse area, and 3. the pulse width. Typically, only
the pulse height is used for analysis. In case of a
DNA analysis, the pulse area of the DNA parameter
may be selected in order to discriminate cell
doublets from single cells, which show up in the
same DNA peak.
The electrical low pass filter smoothes the signals
from the detectors by averaging the signal over a
given time. This feature is only required for lamp
light sources (not realized for the CyFlow
®
space).
Light beams from different lasers are directed to
different positions (spots) of the flow cuvette (please
refer to page 6). Therefore, all laser derived signals
from a laser at a spot other than spot no. 1 need to
be analyzed with a time delay of 50 µsec (2-
Wavelengths Signal Delay activated, see Fig. 5)