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  H301-EC-LG-BL 

User Manual 

   

REV 03

 

www.oko-lab.com 

   

 

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1

 

 

H301-EC-LG-BL 

 

Compatible with the following Z-Piezo stages 

Compatible with the following Okolab Controllers 

 

LEICA super Z Galvo stage (SP5/SP8) 

 

 

H301-T-UNIT-BL-PLUS 

 

UNO-COMBINED-CONTROLLER 

 

H401-T-DUAL 

 

Содержание H301-EC-LG-BL

Страница 1: ...REV 03 www oko lab com Page 1 H301 EC LG BL Compatible with the following Z Piezo stages Compatible with the following Okolab Controllers LEICA super Z Galvo stage SP5 SP8 H301 T UNIT BL PLUS UNO COMBINED CONTROLLER H401 T DUAL ...

Страница 2: ... Sample Holders 4 3 Insertion of the Sample Feedback Temperature Sensor 5 4 Insertion of Sample Holder into Chamber 5 5 Working with 35 or 60 mm Petri Dish 5 6 Working with 1x3 and 1x2 chamber slides 7 7 Connecting the Gas Supply 8 8 Working with Perfusion 8 9 Connecting the Chamber with Super Z Galvo stage 9 10 Cleaning 10 ...

Страница 3: ...dded temperature sensor Heated glass lid with Indium Tin Oxide ITO conductive coating and embedded temperature sensor Total chamber weight about 130 g For details see the table at page 4 Distance between focal plane and upper side of the glass lid Chamber top view Lid Chamber Base Sample Holder Order Separately Figure 1 H301 EC LG BL Components and Dimensions ...

Страница 4: ...er H301 EC LG 1xLABTEK II 1 Lab TeK II1 x2 chambered cover glass holder H301 EC LG 1xGS For 1 standard chamber slide holder H301 EC LG 1x35 For 1 35mm Petri dish holder H301 EC LG 1x60 For 1 60mm Petri dish holder H301 EC LG 1xLABTEK For 1 Lab Tek 1 x2 chambered cover glass holder 1xLABTEK II M single For 1 Lab Tek II 1 x2 chambered cover glass holder Figure 2 Available sample holders H301 EC LG B...

Страница 5: ...dedicated opening Figure 3 Insertion of the temperature sensor inside the chamber 4 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by 4 screws Slotted Pan Head Screw M2x3 To intro duce a sample holder with the proper orientation and to screw it to the chamber see Figure 4 Captive Screw Figure 4 Introduction of the Specimen Holder inside the C...

Страница 6: ...H301 EC LG BL User Manual REV 03 www oko lab com Page 6 A A 1 2 Figure 5 Plate adapter for 35mm Petri dish 2 1 A A Figure 6 Plate adapter for 60 mm Petri dish ...

Страница 7: ...with 1x3 and 1x2 chamber slides Flat springs prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder A A 1 2 Figure 7 Plate adapter for Lab TeK 1 x2 chambered cover glass 1 2 A A Figure 8 1 Plate adapter for Lab TeK II1 x2 chambered cover glass ...

Страница 8: ...y pushing silicon tubing onto brass opening Gas Input Figure 9 Connection with gas supply 8 Working with Perfusion The H301 EC LG BL features 4 perfusion holes on the side for the insertion of perfusion tubing up to 2 0 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M2 5x3 Remove small screws as nec essary before introducing perfusion tubing Figure 10 shows ...

Страница 9: ...C LG BL fits on the Super Z Galvo stage It can be screwed on the Galvo Stage as shown in Figure 11 place the chamber on the stage and screw the four slotted head pan screws Screws housings are indicated with letter A in Fig ure 11 Captive Screw A A Figure 11 How to fix the H301 EC LG BL on the Super Z Galvo stage ...

Страница 10: ...he chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 EC LG BL_User Manual Rev 03...

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