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H201-MINI

User Manual

April 2015

www.oko-lab.com

Sample Holders

2.1

Available Sample Holders

The following sample holders are available.

NOTE:

Please contact

[email protected]

if you cannot find the sample holder you are looking for. We are constantly

adding new inserts to the list.

1x35-M single

#1 35mm Petri-dish

1xGS-M single

#1 1x3in. chamber slide

1xLABTEK-M single

#1 Lab-Tek 1in.x2in. chambered cover glass

1xLABTEK-II-M single

#1 Lab-Tek II 1in.x2in. chambered cover glass

1x60-M-single

#1 60mm Petri-dish

1x35-M single

For #1 35mm Petri-dish holder

1x60-M single

For #1 60mm Petri-dish holder

1xGS-M single

For #

1 1”x3” chamber slide holders

1xLABTEK-M single

For

#1 1”x2”

Labtek

chambered cover glass holder

1xLABTEK-II-M single

For #

1 1”x3”chamber

slide and

#2 35mm petri dishes

Figure 2.Available sample holders.

Insertion of the Sample Feedback Temperature Sensor

Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201-MINI (see Figure 3,

Frontal and 3D views).

1.Frontal view

2.3D view

Temperature Sensor dedicated opening

Figure 3. Insertion of the temperature sensor inside the chamber

Содержание H201-MINI

Страница 1: ...ual April 2015 www oko lab com Page 1 H201 MINI Compatible with the following XY stages Compatible with the following Okolab Controllers Physik Instrumente P 737 1SL 2SL 5SL It fits in any MW plate holder H201 T BL PLUS ...

Страница 2: ...s 4 3 Insertion of the Sample Feedback Temperature Sensor 4 4 Insertion of Sample Holder into Chamber 5 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 5 6 Working with 1x3 and 1x2 chamber slides magnetic locks 7 7 Connection of the Gas Supply 8 8 Working with Perfusion 8 9 Connection of the Chamber with XY stage 9 ...

Страница 3: ...onents and dimensions H201 MINI includes the following components Chamber main body Glass lid Distance between focal plane and upper side of the glass lid Chamber top view Lid Chamber Base Sample Holder Order Separately Figure 1 H201 MINI Components and Dimensions ...

Страница 4: ...60 M single 1 60mm Petri dish 1x35 M single For 1 35mm Petri dish holder 1x60 M single For 1 60mm Petri dish holder 1xGS M single For 1 1 x3 chamber slide holders 1xLABTEK M single For 1 1 x2 Labtek chambered cover glass holder 1xLABTEK II M single For 1 1 x3 chamber slide and 2 35mm petri dishes Figure 2 Available sample holders 3 Insertion of the Sample Feedback Temperature Sensor Insert the Sam...

Страница 5: ... 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 5 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Magnetic Locks for 35 and 60 mm petri dishes Included in the corresponding sample holder Figure 5 Magnetic locks for 35 and 60 mm dis...

Страница 6: ...ock NOTE Spacing rings are included with sample holder Grainer Petri35x10 Ibidi μ Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 7 Ring selection scheme for 35 mm dish MatTek P50G X 30 X Grainer Petri 60x15 Willco60x15 BD Falcon 60x15 Ibidi μ dish 50mm low Corning 60x15mm Figure 8 Ring selection scheme for 60 mm dish ...

Страница 7: ...agnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 9 Magnetic lock for 1 x 3 chamber slide 1 3 2 Figure 10 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Страница 8: ...ng silicon tub ing onto brass opening Gas Input Figure 11 Connection with gas supply 8 Working with Perfusion The Chamber H201 MINI features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use screws plug M3x3 THD Remove small screws plug as necessary before introducing perfusion tubing Figure 12...

Страница 9: ...fix the chamber on the stage follow the steps shown in Figure 13 1 Place the chamber on the stage See Image 1 of Figure 13 2 Tilt the chamber and slide it towards the spring until it snaps in place See Image 2 of Figure 13 3 Image 3 of Figure 13 shows the chamber installed on the XY Stage Springs 1 2 3 Figure 13 Connection of the Chamber with XY Stage ...

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