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ROUBLESHOOTING

Incorrect operations are often mistaken for malfunction. If you think that there is something wrong with a

component, see the troubleshooting scheme below. If the problem persists even after troubleshooting as

described below, please ask for Okolab support

Symptom

Probable cause

Remedy

Device off

Supply cable disconnected

Properly connect the cable

Supply cable damaged

Substitute the cable

No temperature displayed or

“nan"

Thermocouple cable disconnected

Properly connect the cable

Thermocouple cable damaged

Contact Okolab to receive assistance

Water Bath cable disconnected

Properly connect the cable

Water Bath cable damaged

Contact Okolab to receive assistance

Chamber Base and Lid

are cold

Wrong set point value

Change the set point

Thermostat not communicating

Properly connect the 3Pole/RS232 cable

(Thermostat/T-Unit)

Sensor out of the reference well in

“Sample Control Mode”

Sensor out of the reference well in “Sample

Feedback

Mode”

No water circulating

Tube clamps closed: open them.

Lauda Closing Plug is mounted: open the pump

connection removing it.

For Basic version. The setting knob is turned

clockwise: turn it anticlockwise. Check the

Water-Bath manual

Narrowing, throttling, kinking of tubing,

and formation of air bubbles

Check all connections. Properly place and fix

tubings

No temperature read

The T-Unit is off

Switch the T-Unit on

The precision fine wire thermocouple is

disconnected

Control thermocouple connections

The precision fine wire thermocouple is

damaged

Contact Okolab to receive assistance

Alarm triggers

Sample temperature (in sample feedback

mode) or Water Bath temperature (in

chamber feedbacjìk mode) is far to the set

point

Check all connections, make sure that the

thermocouple is correctly positioned in the

reference well and that the incubating chamber is

closed

(if working in “Sample feedback

Mode)

Chamber Windows

Condensation

Direct cold air on the chamber

Avoid direct cold air on the chamber

Condensation after chamber opening

Wait some minutes and it will disappear

I check the previous

troubleshooting but I cannot

solve the problem

Contact Okolab to receive assistance

Содержание Bold Line H101-CRYO-BL

Страница 1: ...1 H101 CRYO BL Manual ...

Страница 2: ...2 This page was left blank ...

Страница 3: ...13 6 USER INTERFACE 14 6 1 Home page 14 6 1 1 Colors meaning 14 6 1 2 Changing Set Points 15 6 2 Settings 15 6 2 1 Temperature 15 6 2 1 1 Control Mode 15 6 2 1 2 Objective Heater used only in exceptional cases 18 6 2 1 3 Thermal Cycles 19 6 2 1 4 Status 21 6 2 1 5 External Sensors 22 6 2 1 6 Digital Input 22 6 2 2 Touch Screen 23 6 2 2 1 Options 23 6 2 2 2 Brightness 23 6 2 2 3 Visual Effects 24 6...

Страница 4: ...29 7 6 Standard Cooling value 30 8 MAINTENANCE OF THE T UNIT 31 9 WATER BATH MAINTENANCE 31 10 SUPPORT 33 10 1 QR Codes for Support 33 10 2 Support Contacts 33 10 3 Web Conference For Assistance And Training 33 11 TROUBLESHOOTING 34 12 TECHNICAL SPECIFICATIONS 35 13 FIGURE LIST 36 ...

Страница 5: ...e to monitor the temperature of a blank sample or the Reference Well located in close proximity to the experimental sample a thermistor to monitor the Room temperature The H101 CRYO BL needs to be connected with the Okolab incubating chamber with one or more plate adapters The H101 CRYO BL can be operated via the touch screen interface OKO TOUCH not included via OKOLAB DATA LOG Software not includ...

Страница 6: ...h helpful suggestions Note Supplies you with important information to successfully setup and use the instrument CE MARKING This symbol indicates a product s compliance with EU legislation PRODUCT DISPOSAL This symbol indicates that this product must not be disposed as urban solid waste This symbol indicates the product production date This symbol indicates the manufacturer data This symbol indicat...

Страница 7: ... and its accessories The products labels can be found on the bottom panel of the T Unit Do not exceed voltage indicated in this manual and on the product label Avoid excessive induction noise static electricity magnetic fields Do not expose this instrument to rain or moisture Prevent throttling and kinking of tubing Check the cables are well fixed to their own connectors so they cannot slip off Th...

Страница 8: ...symbol marks this device IMPORTANT read the Safety Notes before installing using and commissioning this device as the notes contain important information relating to safety and EMC Not following these instructions can result in damage or breakdown of the device and its accessories We reserve the right to make technical variations IN NO EVENT SHALL OKOLAB S R L BE LIABLE FOR ANY DIRECT INCIDENTAL O...

Страница 9: ...ng chamber connected with a 10mm ID black butyl tube isolated to be connected to the colour related connector of the water bath 7 TUBE S Red marked tubing composed of transparent silicon tube 2mm ID to be connected to the colour related connector of the incubating chamber connected with a 10mm ID black butyl tube isolated to be connected to the colour related connector of the water bath 8 1 24V DC...

Страница 10: ...Bath CRYO connector 5 USB port 6 General Purpose Input Output GPIO port 7 NTC Thermistor port 8 Gauge Thermocouple port 5 H101 CRYO BL INSTALLATION AND USER GUIDE The following instructions will guide you through a quick installation For safe operation of the unit it is absolutely necessary to read carefully all the instructions and safety notes ...

Страница 11: ...reen port labeled Reference Temperature located on the rear panel of the T Unit see Figure 2 When operating the device in Sample Feedback Mode place this thermocouple in a Reference Well filled with water Make sure that the end of the Fine Gauge Thermocouple is fully immersed Tip The Fine Gauge Thermocouple may be placed into a Reference Well if running the Sample Feedback Mode see paragraph 6 2 1...

Страница 12: ...ect the 24V DC Power adapter to the T Unit 7 To switch the T Unit on use the power button on OKO TOUCH A small green LED light will blink on the front of the T Unit This means that the system is initializing Once the system has been initialized the LED on the T Unit will no longer blink and will be steadily on The OKO TOUCH interface will display its homepage and the indicator light on the tempera...

Страница 13: ... connection a b The Gas Controller must stack on top of the T Unit by lining up the bus ports located on the top and bottom surface of each When the units are properly connected the Temperature CO2 and O2 parameters will appear on the OKO TOUCH Home page If not all the parameters appear on OKO TOUCH Home Page then the Control Units are not properly connected Please check that the bus ports are pro...

Страница 14: ... points NO actions on your part are required The Yellow light will also appear every time you change the Temperature set point The ORANGE indicator means that the current gas concentration is not correct and its value is out of the set tolerance see section ALARMS in paragraph 6 2 2 5 While giving time to the system to recover from this out of range value please double check if there may be reason...

Страница 15: ...pen where you can press to adjust the temperature to the desired value from 3 C above ambient to 60 C Press Set to save the new set point or Cancel to undo Figure 7 How to change the Temperature set point 6 2 1 Temperature Temperature Settings are adjusted by following the two steps below look at the touching finger Figure 8 Temperature settings 6 2 1 1 Control Mode The Temperature Control can run...

Страница 16: ...cimen therefore cannot be considered an active thermal control on the sample Tip Advantages of this Control Mode are fast experiment start up and no specific action required in multi user applications The disadvantage is that it needs calibration between water bath and the specimen The temperature difference between the water bath and the sample sensor depends on many factors like the ambient temp...

Страница 17: ...rature sensor lid To be used in specimen feedback in a 35 mm Petri Dish SENSOR LID 60 Temperature sensor lid To be used in specimen feedback in a 60 mm Petri Dish SENSOR LID GS Temperature sensor lid To be used in specimen feedback in chamber slides and chambered cover glass Table 1 Sensors lid codes Figure 12 SENSOR LID 35 For 35 mm Petri dish Tip Advantages of Sample Feedback Control Mode are di...

Страница 18: ... in a holding state until the incubator is closed and its environment is in a steady state This can be achieved using the Open Incubator procedure 6 2 1 2 Objective Heater optional Figure 14 shows how to enable disable the Objective Heater OBJ COLLAR The Objective Heater is disabled by default Read this paragraph only if your system is equipped with Objective Heater OBJ COLLAR Figure 14 Objective ...

Страница 19: ...the temperature is set at 37 C the Objective Heater is kept at 40 C in order to maintain the sample at the desired temperature while imaging with an oil immersion objective To accept this offset you can press USE FACTORY and return to the Homepage Alternatively the following options are available 8 MANUAL Selecting MANUAL allows searching for the best offset value manually step by step See Figure ...

Страница 20: ...form see Figure 18 Figure 18 Thermal cycles settings Tip The system switches from a higher to a lower temperature by dissipation hence it requires some time the amount of time required depends on several factors such as control mode room temperature and set points Please keep this in mind when setting the time between the two set points Once you have adjusted the settings a Cycle Summary page will...

Страница 21: ... or starting a new thermal cycle while a different one is still running will open the window in Figure 21 You can press Abort Cycle to modify it or start a new one Pressing View will open the current thermal cycle settings Figure 21 Running Cycle window 6 2 1 4 Status To open the Status page press the relative icon as shown in Figure 22 The Settings Temperature page will open next select Status On...

Страница 22: ...overheating of the chamber When running in Chamber Feedback Mode the green Fine Gauge Thermocouple can be placed in any area of the system to monitor temperature in that area With this setup you can monitor both temperatures read by the thermistor T Room and by the thermocouple Free Sensor see Figure 42 Figure 23 External sensors subpage 6 2 1 6 Digital Input Digital Input feature is available onl...

Страница 23: ...f the X axis time duration of the graphs displayed in the main page and the buzzer sound frequency Figure 26 Touch Options Press and slide your finger along the setting bars or simply click to set desired values then press Save Tip Recommended value for the chart history length is 60 or 120 minutes 6 2 2 2 Brightness This page allows you to increase reduce the brightness of the touch screen scroll...

Страница 24: ...p LED will never illuminate if you select On Alarm the Top LED will illuminate only when theH401 T CONTROLLER is in Alarm Status see paragraph 6 1 1 if you select Always the Top LED will always illuminate 6 2 2 4 Date Time The first time you turn the system on it is important to set date and time use the and to set correct date time and then press Save Figure 29 Touch screen settings Date and Time...

Страница 25: ...s stationary state if Temperature Deviation from set point is 1 C or greater i e if set point temperature is 37 C and the temperature reaches a value equal or less than 36 0 C or equal or more than 38 0 C for a period of time equal or longer than 15 minutes Temp time set in this example then the system triggers an Alarm 6 2 2 6 Logging To use this system feature refer to the Data Logging quick ins...

Страница 26: ...the digits in Glance Mode follows the color coding reported in paragraph 6 1 1 Pressing on Glance Mode you can change the data visualization format on the Homepage that turns into brief information about the state of the controller and the attached ones Figure 33 Glance Mode with temperature controller only Figure 34 Glance Mode with temperature controller CO2 O2 gas controller and active humidity...

Страница 27: ...in terms of thermal stability and fast temperature recovery Please avoid modifying the water bath factory parameters Some parameters alterations could make the system bad functioning or could induce the system failure Please carefully read the following instructions in case some control parameters in thermostatic bath display were accidentally garbled Please follow the next scheme to set the therm...

Страница 28: ...28 ...

Страница 29: ... of circulation pump is automatically set at the standard value 2 This value 2 ensures good circulation and thermal uniformity in the incubation chamber If you want to VERIFY or modify this value please follow the next scheme Tip If the recirculation is impeded or reduced the Stage Incubator will not be evenly warmed up Avoid narrowing throttling kinking of tubing and formation of air bubbles to e...

Страница 30: ...g mode To alter the cooling setting mode press the key on the left The display flashes approx 4 sec During this time start to set the operating mode with one of the keys 0 Refrigeration system OFF 1 Refrigeration system ON 100 capacity Only cooled system A automatic operation ...

Страница 31: ...fic know how Changes of the CAL parameters can worse the incubating system performances in terms of temperature stability If the calibration parameter CAL is accidentally varied please check with a calibrated temperature sensor or a mercury thermometer if the actual water temperature is the same displayed otherwise contact our service centre 8 MAINTENANCE OF THE T UNIT To maintain proper T Unit op...

Страница 32: ... a specific gravity of 1 092 at 21 C the specific gravity can be easily checked with the help of a hydrometer Add pure water or glycol in order to recreate the right mixing ratio from time to time The time saving alternative if you are sure to work at 37 for long time is to refill the tank with distilled water only if the liquid evaporates in this case the ratio will be modified and the liquid wil...

Страница 33: ... For COMMERCIAL SUPPORT lanzaro oko lab com Phone 39 081 806 2624 Fax 39 081 876 4410 Mobile 39 348 9680717 For TECHNICAL SUPPORT sibillo oko lab com Phone 39 081 806 3470 Mobile 39 348 9680718 Okolab S r l Via A Olivetti 1 80078 Pozzuoli NA Italy In case you requested on line support and or web conference assistance for system assembling and experiment support you received a web cam Please follow...

Страница 34: ...r out of the reference well in Sample Feedback Mode No water circulating Tube clamps closed open them Lauda Closing Plug is mounted open the pump connection removing it For Basic version The setting knob is turned clockwise turn it anticlockwise Check the Water Bath manual Narrowing throttling kinking of tubing and formation of air bubbles Check all connections Properly place and fix tubings No te...

Страница 35: ...1 C Accuracy 0 2 C in Sample Mode and in Cage Mode if room temperature remains within 1 C Regime temperature time Depends on the control mode about 30 min in chamber mode feedback Operating Temperature 0 C 55 C Storage Temperature 5 C 60 C Operating Humidity 0 70 Power Consumption 24 V DC 18 W max Weight 1 55 Kg Water Bath Supply Voltage 115V 60 Hz 230V 50 Hz Water Bath Power Consumption 2 2 kW Wa...

Страница 36: ...on Page 19 Figure 16 Objective Heater Manual Offset 19 Figure 17 Thermal Cycles Subpage 20 Figure 18 Thermal cycles settings 20 Figure 19 Thermal cycle Summary 21 Figure 20 Cycle Start Time 21 Figure 21 Running Cycle window 21 Figure 22 Status page 22 Figure 23 External sensors subpage 22 Figure 24 Digital input 23 Figure 25 Touch screen settings 23 Figure 26 Touch Options 23 Figure 27 How to ente...

Страница 37: ...action of the purchaser that discord from instructions listed in the operation manual This warranty does not cover or involve any other equipment that may be used along with the Okolab System i e mini incubators any gas tank etc whose usage should be considered independent and performed according to their own operational instructions Okolab S r l warrants only the parts manufactured by it will as ...

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