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NuAire LabGard D NU-543-400, Руководство пользователя

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OM0283 

Page 4 of 70 

Rev 2 July/2021 

LabGard® ES Energy Saver Class II, Type A2 Laminar Flow Biosafety Cabinet 

Models NU-543-300/400/500/600 Operation and Maintenance Manual 

 

Table of Contents 

Section No. 1 ..................................................... General Information 
Section No. 2 ..................................................... Models and Features 
Section No. 3 ..................................................... Warranty 
Section No. 4 ..................................................... Shipments 
Section No. 5 ..................................................... Installation Instructions 

5.1 ..................................................................... Location 
5.2 ..................................................................... Set-up Instructions 
5.3 ..................................................................... Testing Methods and Equipment 

Section No. 6 ..................................................... Operating the NU-543 

6.1 ..................................................................... 

FlowGard™

 Control System 

6.2 ..................................................................... Operating Guidelines 
6.3 ..................................................................... Operating Sequence 
6.4 ..................................................................... Ergonomics 
6.5 ..................................................................... Cleaning Procedures 
6.6 ..................................................................... Hazardous Drug Contamination Procedures 

Section No. 7 ..................................................... General Maintenance 

7.1 ..................................................................... Decontamination 
7.2 ..................................................................... Fluorescent Lamp Bulb Replacement 
7.3 ..................................................................... HEPA Filter/Motor Replacement 
7.4 ..................................................................... Sliding Window Replacement and Manual Adjustment 
7.5 ..................................................................... Airflow Control System Setup and Calibration 
7.6 ..................................................................... HEPA Filter Leak test 
7.7 ..................................................................... Airflow Smoke Pattern Test 
7.8 ..................................................................... Site Installation Assessments 
7.9 ..................................................................... Cleanliness Classification Test for Pharmacy Application 
7.10 ................................................................... Main Control Board Description and Replacement 
7.11 ................................................................... Digital Airflow Sensor Description and Replacement 

Section No. 8  .................................................... Error Messages, Troubleshooting and Option-Diagnostics 
Section No. 9 ..................................................... Remote Contacts 
Section No. 10 ................................................... Optional Equipment 

10.1 ................................................................... Ultraviolet Light 

Section No. 11 ................................................... Electrical/Environmental Requirements 
Section No. 12 ................................................... Disposal and Recycle 

 

Manual Drawings 

ACD-16371 ...................................... NU-543 Airflow Schematic 
BCD-16372 ...................................... NU-543-300 Specification Drawing 
BCD-16373 ...................................... NU-543-400 Specification Drawing 
BCD-16374 ...................................... NU-543-500 Specification Drawing 
BCD-16375 ...................................... NU-543-600 Specification Drawing 
BCD-16300 ...................................... Drain Valve Installation 
BCD-16377 ...................................... Front Label 
BCD-16509 ...................................... NU-500 Series Auto Decontamination Set-Up 

 

Assembly Drawings 

BCD-16633 ...................................... Base Stand Assembly 
BCD-16301 ...................................... Control Center and Front Decorative Panel Assembly 
BCD-16376 ...................................... Sliding Window Assembly and Adjustment 
BCD-16299 ...................................... Base Cabinet Assembly 

 

Electrical Schematics 

BCD-16378 (sheet 1 and 2) ............. NU-543-300/400/500/600 Electrical Schematic 

 

Содержание LabGard D NU-543-400

Страница 1: ...Cabinet Models NU 543 300 400 500 600 Bench Console Operation and Maintenance Manual July 2021 Revision 2 NU 543 300 Series E Nu 543 400 500 600 Series D Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 ...

Страница 2: ...ion from particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other reference and guideline materials are available through the following web sites www hc sc gc ca www cdc gov od ohs www absa org www cabs acsb ca www ebsaweb eu www inspection gc ca www who int www biosafety ...

Страница 3: ...wings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to this manual In case this manual and or test report is lost or misplaced NuAire retains a copy in our files A replacement copy can be obtained by calling or writing NuAire Inc stating the model number...

Страница 4: ...moke Pattern Test 7 8 Site Installation Assessments 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement 7 11 Digital Airflow Sensor Description and Replacement Section No 8 Error Messages Troubleshooting and Option Diagnostics Section No 9 Remote Contacts Section No 10 Optional Equipment 10 1 Ultraviolet Light Section No 11 Electrical En...

Страница 5: ... Airflow Schematic and the application of environmental controls as required in the field of biological research or chemical containment The LFBSC when used with proper technique is an effective primary engineering control in obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to ...

Страница 6: ... while specific agents are used o Which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operating instructions close to the cabinet so that safety instructions and important information are always accessible Should you encounter problems that are not detailed adequately in the operating instruc...

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Страница 8: ...283 Page 8 of 70 Rev 2 July 2021 2 0 Models and Features The model NU 543 LabGard ES Class II Type A2 Laminar Flow Biosafety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m ...

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Страница 13: ...eturning any item for any reason contact NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned to you as refused shipment or collect freight 4 0 Shipments NuAire takes every reasonable precaution to assure that your LabGard ES cabinet arrives without damage Motor carriers are carefully selected and shippin...

Страница 14: ...maintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is inaccessible such as a conduit hardwired connection then an appropriate warning label should be applied near the cabinets on off switch to indicate the circuit breaker on the power distribution panel should be used A...

Страница 15: ...in valve is desired NOTE CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS OF DRAIN VALVE INSTALLATION remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until secure with the valve stem for handle on the left side Re install handle to valve stem Adjust the cabinet on bench to provide a 2 51mm ov...

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Страница 17: ...per cabinet size reference Electrical Environmental Requirements It is recommended that power to the cabinet whether hardwired or plug connected be on its own branch circuit protected with a circuit breaker at the distribution panel near the cabinet A surge protector is strongly recommended if you are experiencing power related faults NOTE THIS CABINET CONTAINS ELECTRONIC BALLASTS FOR THE FLUORSCE...

Страница 18: ...ocity test Inflow velocity test Airflow smoke patterns Site installation assessment tests The testing methods and equipment required are specified on the factory inspection report included with this manual see insert in back cover NOTE IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR TESTING BIOSAFETY CABINETS SEE INSERT ...

Страница 19: ...d Height Minimum for Transport Width Depth with Armrest Removed 60 7 8 1546 59 1499 41 5 8 1057 31 7 16 799 60 7 8 1546 59 1499 53 5 8 1362 31 7 16 799 60 7 8 1546 59 1499 65 5 8 1669 31 7 16 799 60 7 8 1546 59 1499 77 5 8 1972 31 7 16 799 Work Access Opening Inches mm Standard Opening Height Optional Standard Inflow Velocity 10 254 8 203 105 FPM 53 m s 10 254 8 203 12 305 105 FPM 53 m s 10 254 8 ...

Страница 20: ...C Motor Controller that provides automatic compensation constant volume control for both filter loading and line voltage variances Monitor and display airflow velocity performance via Intelliflow Monitor Display time of day and temperature The LabGard ES NU 543 offers the latest digital microprocessor design technology for improved cabinet performance and safety The FlowGard control system integra...

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Страница 22: ...arm condition and remain indicating until the alarm condition is cleared A blinking red alarm LED indicates a power interruption has occurred 6 1 2 7 Audible Alarm Silence Key The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15 minutes After 15 minutes if the alarm condition still exists the audible alarm will again sound The audible alarm silence k...

Страница 23: ...g window is raised an audible and visual alarm will occur but may be silenced by pressing the alarm silence key Airflow readings will not be displayed during the warm up period Once the warm up period is complete airflow readings and all system functions will operate and be displayed The top line will show the items being displayed along with the time of day The center of the display will show the...

Страница 24: ...alarm description Audible alarms will produce an alarm tone for 30 seconds then ring back for 2 seconds of every 5 seconds Pressing the alarm silence key will silence the audible alarm for 15 minutes initially then will start the ring back function again The bottom of the display or message area will indicate alarms errors or other notable conditions Since the message area is limited to one line o...

Страница 25: ...change Press key to back up one level or repeated times to return to normal run or standby mode 6 1 8 2 Time Menu The time menu contains both time of day and auto timer duration Upon selecting the time menu from the main menu the display will indicate the following Time of Day o Press ENT key to select time of day The display will indicate the following o Press ENT key to advance through the follo...

Страница 26: ...splayed is provided by the airflow sensor in the cabinet s exhaust airstream The temperature displayed may indicate a slightly higher value than the laboratory room temperature due to some heat generation of the cabinet itself However the temperature may be adjusted by an offset or deviation from the indicated temperature plus or minus in tenths of a degree calibration procedure by the following P...

Страница 27: ...ng should be evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should also be planned to minimize the number of times an operator s hands and arms must enter and leave the air curtain at the open face The ideal situation is to have everything needed for the ...

Страница 28: ... precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environment should not be discontinued Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until surface decontaminated Trays of discard pipettes must be ...

Страница 29: ...ills Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet away from open face as possible 6 3 4 Air Purge Additional purging of the workspace without user activity should be allowed ...

Страница 30: ...ure feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments Forearm elbow support The cabinet is provided with a non metallic forearm support on the work access opening Periodic mini breaks during work practice should be taken resting forearm to avoid stress and fatigue Elbow rests that can provide support for particular work...

Страница 31: ...II LFBSC can be found in the 2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal blower on b Open the hinged or sliding view screen and secure in the full open position With the view screen in the full open position personnel protection is compromised and a full faced HEPA filter...

Страница 32: ...een microbiologically decontaminated is known to be biologically clean or known to be chemically inert Surface disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protection Equipment Required Decontamination using hazardous gas vapor or mist must be performed in accordance with the specifications of NSF ANSI 49 Annex G EN12469 Annex J or applicable n...

Страница 33: ...the control center Reconnect power to the cabinet The outlets and blower can then be activated 9 Seal front and top openings using plastic and tape BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS VAPOR OR MIST 10 Perform decontamination procedure 7 1 2 Auto Decon The Auto Decon process is intended to be used with any automated cabinet decontamination pro...

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Страница 35: ...dges of the front window sealed with a low residue tape 6 Tubing connected to the inlet and outlet of X10 7 Insertion of Hydrogen Peroxide H2O2 sterilant cup and desiccant cartridge to X10 8 Current BSC process selected Decontamination Process 9 The X10 will perform one 1 Preheat Dehumidification Decontamination and Aeration after start of the program 10 Preheat The cabinet is heated to 31 C 11 De...

Страница 36: ...ntamination system and cabinet Total duration is the total automated decontamination cycle time so both the cabinet Auto Decontamination time duration and the automated decontamination system cycle time are sequenced and run together Blower run time is the length of time the blower will run during and of the 4 possible blower start times Blower start schedule is the start time of 4 possible blower...

Страница 37: ...essary to remove the lamp assembly 1 Switch cabinet light switch off 2 Remove the screws at each upper side of the control center and allow the control center to rotate down resting on the safety straps 3 The lamp is now directly exposed for replacement 4 The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting out the lamp 5 Reverse the procedure to ...

Страница 38: ...d lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest window position will require the armrest to be removed Step 3 Filter Removal It is not always necessary to replace both the supply and exhaust filters at the same time If during the course of certifica...

Страница 39: ...3mm x 3 76mm 20 508mm x 14 356mm x 11 1 2 292mm Filter Manufacturer Camfil Farr Camfil Farr NU 543 400 NuAire Part Number A 980979 01 A 980957 12 Filter Size 21 533mm x 44 1118mm x 3 76mm 20 508mm x 22 559mm x 11 1 2 292mm Filter Manufacturer Camfil Farr Camfil Farr NU 543 500 NuAire Part Number A 980979 02 A 980957 13 Filter Size 21 533mm x 56 1422mm x 3 76mm 20 508mm x 30 762mm x 11 1 2 292mm Fi...

Страница 40: ... top of the hood Tighten the spring loaded bolts 4 places depressing the gasket material by 1 8 inch 3mm Step 5 Motor Blower Assembly Removal a It is recommended that the motor blower to be removed as a single unit To remove disconnect electrical connections to the motor remove the HEPEX pressure plenum and unbolt the motor blower assembly from the roof of the cabinet 4 places Always inspect the r...

Страница 41: ... menu perform the following Press and hold key then press ENT and keys sequentially releasing the key after the 3 key sequence The following service parameter display should appear Navigation through the service parameter menu is the same as the operator menu Press on keys to select a menu item which will be highlighted inversely Menu selection moved in a round robin pattern Press ENT key on a sel...

Страница 42: ...with clean filters The maximum rpm is a known fixed value based on the DC ECM motor used The difference between the maximum and current rpm determines the filter life percentage The filter life percentage starts at 100 and as the current rpm increases due to filter loading the filter life decreases Inflow Velocity Default values Low Limit 90fpm Setpoint 105fpm High Limit 120fpm Filter Life Filter ...

Страница 43: ...e adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been NuAire s experience that the filters may not load evenly choke adjustments may be necessary for proper cabinet airflow balance and performance DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration The DC voltage may be measured using a digital v...

Страница 44: ...ease blower speed Greater than 110LFPM 56 m s First decrease blower speed to achieve the exhaust excess then close the choke plate to achieve the balance In this fashion the downflow should remain nearly constant i e what the reduced speed took away the choke plate restores Note The choke plate adjustment requires a standard blade screwdriver To adjust loosen the liquid tight fitting around the ch...

Страница 45: ...fset value may be positive or negative as needed Once desired offset value is achieved press ENT key to accept Press to exit 7 5 4 4 Alarm Verification If desired the alarm setpoint can be verified by entering into the alarm verification menu The airflow alarm is always active in this menu so if the blower is turned on in this menu an airflow alarm will be active until the airflow is above the low...

Страница 46: ...ss ENT and keys sequentially releasing the key after the 3 key sequence The following service parameter display should appear Press and to select calibration Select and press ENT key for blower speed calibration Select and press ENT key for Nite Care Adjust blower speed with increase and decrease as needed Press ENT key to accept Nite Care blower speed calibration Service Parameters Configuration ...

Страница 47: ...2 5 318 12 5 318 12 5 318 18 750 476 18 0 457 17 750 451 17 5 445 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within 20 or 16fpm 0 08m s whichever is greater factory test or 25 or 16fpm 0 08m s whichever is greater field test from the average downflow velocity B Inflow Measurement a Recomme...

Страница 48: ... 102 8 158 207 12 316 313 16 474 481 20 632 524 24 790 630 28 948 735 33 106 841 37 264 946 41 422 1052 45 580 1158 49 738 1263 53 896 1369 58 054 1475 62 212 1580 66 375 1686 Number of Readings Average Velocity of Constricted Area ft min ms 1 Average Velocity of Constricted Area fpm m s 2 X Constricted Access Area ft2 m2 3 Constricted Area Volume CFM m3 s 4 Constricted Area Volume CFM m3 s 5 10 2...

Страница 49: ...ed and should be removed If an air gap exhaust transition is provided the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux All exhaust blowers fans should be turned off during the check The exhaust sensor shroud can also be removed for the exhaust filter integrity check Replace the exhaust sensor shroud immediately aft...

Страница 50: ...nt where an audible and or visual alarm will activate to signify unfavorable operating conditions within the Biosafety cabinet and or the remote exhaust blower and canopy connection performance 7 8 1 Sash Alarm Step 1 With sash alarm switch enabled raise the sliding sash 1 2 5cm above the manufacturer s designated sash height for normal operation Verify that the audible visual alarm activates soun...

Страница 51: ... test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Take 5 test points in 1 minute intervals on a grid in a horizontal plane as measured approximately 6 inches 152mm above the worksurface The grid location is designed as the workzone center point and ea...

Страница 52: ... 2 2 AMPS 1 AMP 2 NU 543 400 12 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 NU 543 500 15 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 NU 543 600 15 AMPS 3 AMPS 2 2 AMPS 1 AMP 2 7 10 3 Main Control Board Replacement Note All setup and calibration data will be lost the memory reinitialized to the default values and all control functions reset to an initial cabinet power condition If possible before the main control board replace...

Страница 53: ...t The airflow sensor function utilizes two thermistors that provide a constant current source One thermistor is a reference that uses a very low current source The other thermistor is the airflow measurer that uses a very high current source As airflow passes over the thermistors the airflow removes heat from the thermistor measuring airflow The loss of heat from the thermistor causes the voltage ...

Страница 54: ...mps Check voltage coming out of main control board to light ballasts Cabinet blower won t turn on Green LED above blower key indicates the blower should be on Airflow Alarm Check blower fuse on main control board Check AC voltage coming out of main control board Check wiring to blower Check blower motor Check DC motor PWM signal on main control board Power on reset Indicates a power interruption h...

Страница 55: ...ely Menu selection moved in a round robin pattern Press ENT key on a selected menu item will either 7 Drill further down the menu tree 8 Allow editing of the item value which will now be highlighted o Press or key to change the selected value o Press ENT key to accept the change o Press key to cancel the change Press key to back up one level or repeated times to return to normal run or standby mod...

Страница 56: ... on via the outlet key If the outlet power sync is active the blower must be actively running for the outlet power to turn on 8 2 2 Function Options Disable Intelliflow If this function is active All airflow display and alarm functions are turned off Allow UV light anytime Normally the UV light is interlocked with the window being in the closed position For service purposes only if this function i...

Страница 57: ... occurs The blower will automatically come back on when power is restored If this function is turned off the blower will not automatically come back after a power interruption but would require the user to press the blower key to restart the blower Require Password Normally it is not required to use a password i e 3 keypress sequence of the blower and hidden key If the option is turned on it would...

Страница 58: ...run time is the length of time the blower will run during and of the 4 possible blower start times Blower start schedule is the start time of 4 possible blower start times during the decon cycle Initiate decon cycle provides a short safety check list the must be answered yes before the decon cycle can be initiated Once the Auto Decon cycle is initiated the following display indicates cycle progres...

Страница 59: ...vated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an airflow alarm condition occurs Contact ratings are 250 VAC maximum at 2 Amps 9 3 15VDC Output The 15VDC 100mA output is active when powe...

Страница 60: ...9 June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the cabinet face area 10 1 2 Operation The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch located on the front panel The sliding window is in...

Страница 61: ...system monitors UV light usage hour and when the 5000 hour point is reached The UV light LED indicator will blink fast along with the display message Replace UV light To view hours or reset timer perform the following Press and hold ENT key for 3 seconds until the menu appears Press or keys to select UV lamp hours Press ENT key to highlight hours Press or key simultaneous until hours zero Press EN...

Страница 62: ...rep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus vulgaris 7 480 Serratia marcescens 6 160 Bacillus subtilis 11 000 Bacillus subtilis spores 22 000 Spirillum rubrum 6 160 References a Luckiesh Matthew 1946 Application of Germicidal Ethyemal and Inf...

Страница 63: ...arable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dus...

Страница 64: ...nt Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Panel Painted Steel Control Center Painted Steel Supply Diffuser Aluminum Exhaust Filter Aluminum HEPA Filter Frames Painted Steel Hepex Bag PVC Blower Wheel and Housing Steel Motor Various Steel Pr...

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