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ADDENDUM FOR

SEC-05X

SINGLE ELECTRODE AMPLIFIER

SYSTEM

Information on the revised version of the SEC-05X amplifier – to be

used in conjunction with the SEC-05LX user manual

npi 2010

npi electronic GmbH, Hauptstrasse 96, D-71732 Tamm, Germany

Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240

[email protected]; http://www.npielectronic.com

Содержание SEC-05LX

Страница 1: ...n the revised version of the SEC 05X amplifier to be used in conjunction with the SEC 05LX user manual npi 2010 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49...

Страница 2: ...p mode see also SEC 05LX manual page 24 CC mode Current Clamp mode see also SEC 05LX manual page 24 BR mode Bridge mode see also SEC 05LX manual page 24 EXT mode External mode see also SEC 05LX manual...

Страница 3: ...Amplifiers with x10 Headstage Calibration of SEC Amplifiers with x0 1 Low Voltage Headstage Synchronization of Two or More SEC Amplifier Systems SEC Systems with VCcCC Mode SEC Systems with DHC Mode...

Страница 4: ...y system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the in...

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Страница 25: ...ms VC ERROR VC ERROR GAIN GAIN VC OUTPUT LIMITER VC OUTPUT LIMITER 100 100 50 50 60 60 80 80 90 90 70 70 40 40 30 30 20 20 10 10 OFF OFF RISE TIME RISE TIME ms ms 0 4 0 4 0 2 0 2 OFF OFF 1 1 0 8 0 8 0...

Страница 26: ...roportional and integral which reduces the error considerably theoretically to zero The time constant of the integrator is set with the potentiometer 3 Clockwise time constant is decreased effect of i...

Страница 27: ...essel filter 12 or 24 dB octave with 16 corner frequencies The following 16 frequencies can be set 20 50 100 200 300 500 700 1k 1 3k 2k 3k 5k 8k 10k 13k 20k Hz The position of this switch is monitored...

Страница 28: ...conditions it is necessary to limit the current in the voltage clamp mode e g in order to prevent the blocking of the electrode or to protect the preparation This is possible with an electronic limit...

Страница 29: ...RRENT CLAMP MODE using a discontinuous current injection V VOLTAGE CLAMP MODE using a discontinuous feedback 21 HOLDING CURRENT nA HOLDING CURRENT control and 0 POLARITY SWITCH The current injected th...

Страница 30: ...esets V mode switched voltage clamp mode See also MODE OF OPERATION 20 27 REL Mode Switch and REL mode switch M LED light emitting diode This switch activates the automatic electrode resistance measur...

Страница 31: ...monitoring the recorded potential with a gain of ten 35 CURR SENSITIVITY MONITOR CURRENT SENSITIVITY MONITOR This BNC connector provides eight output voltages 0 7V 1 Volt per switch position correspon...

Страница 32: ...with an on off toggle switch that can be used to ground unused inputs to avoid noise pickup 42 REMOTE REMOTE BNC connector for activating the PENETRATION UNIT See also 10 43 HEADSTAGE INPUT HEADSTAGE...

Страница 33: ...o the discontinuous current injection GROUND PROTECTIVE EARTH GROUND PROTECTIVE EARTH Connectors see also 29 In order to avoid ground loops the internal zero ground signal of the instrument is not con...

Страница 34: ...th 115V AC it must be 1 25A slow CAUTION see also page 2 Always use a three wire line cord and a mains power plug with a protection contact connected to ground Before opening the cabinet disconnect ma...

Страница 35: ...Draguhn A Pfeiffer M Heinemann U and Polder H R 1997 A simple hardware model for the direct observation of voltage clamp performance under realistic conditions J Neurosci Methods 78 105 113 Finkel AS...

Страница 36: ...Polder HR Swandulla D Konnerth A and Lux HD 1984 An Improved High Current Single Electrode Current Voltage Clamp System Pfl gers Archiv 402 R35 Polder HR Swandulla D 1990 Design and optimal tuning of...

Страница 37: ...time constant capacity compensation Juusola M 1994 Measuring complex admittance and receptor current by single electrode voltage clamp J Neurosci Meth 53 1 6 Weckstr m M Kouvaleinen E Juusola M 1992...

Страница 38: ...ng by an antiarrhythmic peptide via protein kinase C activation FASEB J 16 1114 1116 Simultaneous recordings with two SEC amplifiers Haag J and A Borst 1996 Amplification of high frequency synaptic in...

Страница 39: ...aled by infrared guided laser stimulation Science 286 110 113 Eder M Zieglgansberger W Dodt H U 2002 Neocortical long term potentiation and long term depression site of expression investigated by infr...

Страница 40: ...etrich D Clusmann H and T Kral 2002 Improved hybrid clamp resolution of tail currents following single action potentials J Neurosci Meth in press Draguhn A Pfeiffer M Heinemann U and Polder H R 1997 A...

Страница 41: ...s III Voltage clamp problems Biophys J 65 469 491 M ller W and Lux H D 1993 Analysis of voltage dependent membrane currents in spatially extended neurons from point clamp data J Neurophysiol 69 241 24...

Страница 42: ...Mody I 1992 Membrane properties of dentate gyrus granule cells Comparison of sharp microelectrode and whole cell recordings J Neurophysiol 67 1346 1358 Silver R A Traynella S F and Cull Candy S G 199...

Страница 43: ...S J 1985 Optimal voltage clamping with single m icroelectrode In Smith T G Lecar H Redman S J and Gage P W eds Voltage and patch clamping with microelectrodes Chapter 5 The William and Wilkins Company...

Страница 44: ...ch Clamp Technik Spektrum Akad Verl Heidelberg Berlin Oxford Ogden D C ed 1994 Microelectrode techniques The Plymouth Workshop Handbook 2nd edition The Company of Biologists Limited Cambridge Polder H...

Страница 45: ...N FOR THE PASSIVE CELL MODEL FOR SINGLE ELECTRODE SWITCHED AMPLIFIERS VERSION 4 0 npi 2010 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Tel 49 0 7141 9730230 Fax 49 0 7141 9730240 support...

Страница 46: ...brane and the electrodes and capacitances that simulate the capacitance of the cell membrane A switch allows simulation of two different cell types a medium sized cell with 100 M membrane resistance a...

Страница 47: ...Operation Connections Turn POWER switch of the amplifier off a For simulation of an experiment using a suction electrode Connect the BNC jack of the cell model to the BNC connector PEL of the headsta...

Страница 48: ...GASEAL in patch clamp experiments Simulation of intracellular recording Intracellular recordings can be mimicked with one of two cells with different properties Use the 100 M electrode connector 1 Fig...

Страница 49: ...1 Principles of the dSEVC technique are found in 1 2 Looking back In the early eighties when the design of the SEC 1L system was started single electrode clamping began to gain importance beside the...

Страница 50: ...study fe is 80 160 kHz the selected switching frequency of the dSEVC was 30 50kHz calculated range is 25 53 kHz data were sampled at 10 kHz and the current signals have been filtered at 5 kHz These s...

Страница 51: ...to the leftmost position Select a DUTY CYCLE as desired 24 at the front panel Connect the BNC connector ELECTRODE POTENTIAL OUTPUT at the rear panel to an oscilloscope and trigger with the signal at B...

Страница 52: ...n 1 11 page 4 Figure 3 Tuning of the coarse capacity compensation with an electrode resistance100 M in the bath Time course of the signal at ELECTRODE POTENTIAL OUTPUT is shown holding current 1 nA du...

Страница 53: ...11 page 5 Figure 4 Tuning of the coarse capacity compensation Time course of the signal at ELECTRODE POTENTIAL OUTPUT is shown holding current 1 nA duty cycle switching frequency 2 kHz A model cell wa...

Страница 54: ...ation Version 1 11 page 6 Figure 5 Capacity compensation of the electrode in the bath electrode resistance 100 M Current stimulus 1 nA duty cycle switching frequency 2 kHz Current stimulus and electro...

Страница 55: ...n 1 11 page 7 Figure 6 Capacity compensation of the electrode using a model cell electrode resistance 100 M current 1 nA cell membrane 100 M 100 pF duty cycle switching frequency 2 kHz Current stimulu...

Страница 56: ...w tens of pA for patch recordings to the cell Negative current pulses are recommended If you apply positive current pulses be sure only to elicit ohmic responses of the cell membrane i e pulses should...

Страница 57: ...oltage clamp J Neurosci Methods 53 1 6 5 Weckstrom M Kouvaleinen E Juusola M 1992 Measurement of cell impedance in frequency domain using discontinuous current clamp and white noise modulated current...

Страница 58: ...1000 5000 and 10000 sec for the low pass filter To start using the VCcCC mode the amplifier must be tuned accurately in the fast VC mode toggle switch off The holding potential control must be set on...

Страница 59: ...the VCcCC mode fast current stimuli can be used e g to monitor conductance changes Literature 1 Peters F D Czesnik A Gennerich D Schild 2000 Low frequency voltage clamp recording of voltage transients...

Страница 60: ...he membrane potential is fed into a sample and hold electronic If a TTL pulse 5 V is applied to the MODE SELECT TTL SEC 10LX DHC TTL BNC connector the SEC TEC is switched to VC mode The COMMAND INPUT...

Страница 61: ...tor slave instrument s Up to four SEC amplifiers can be synchronized one SEC acting as master and three SEC operating as slaves The position of the toggle switch marked INT MODE SYNC MODE decides whet...

Страница 62: ...junctional conductance between electrically coupled cells with dual whole cell voltage clamp under conditions of high series resistance J Neurosci Methods 187 13 25 M ller A M Bachmann H R Polder S D...

Страница 63: ...re it is recommended for whole cell patch clamp recordings All current related signals have to be divided multiplied by 10 CURRENT DISPLAY XXX pA no decimal point 100 100 pA HOLDING CURRENT XXX pA 100...

Страница 64: ...ecommended for whole cell patch clamp recordings Current related signals CURRENT DISPLAY X XXX pA 100 100 pA HOLDING CURRENT X XX nA 100 1 nA Input sensitivity BNC labeled 1 nA V i e 1V 1nA BNC labele...

Страница 65: ...A 10 V nA corresponds 1 V nA CURRENT DISPLAY shows current divided by ten XX X nA 10 0 corresponds to 100 nA Maximum shown is 199 nA BRIDGE BALANCE Switch in 100 M position XXX M 050 corresponds 5 M M...

Страница 66: ...BRIDGE B mode All inputs must be turned off or disconnected The BRIDGE balance control must be on 000 to avoid incoming disturbances The following systems and front panel elements are working CAP COMP...

Страница 67: ...mplifier work in linear mode either without or with x10 amplification Linear Mode x1 x10 switch x1 The amplifier operates in linear unswitched mode see below current and or voltage are not enhanced LI...

Страница 68: ...CE compensation do not use the potential values recorded from the POTENTIAL OUTPUT mV BNC connector or the value shown at the POTENTIAL display as a measure for the membrane potential since these valu...

Страница 69: ...often lower due to uncompensated stray capacities and nonlinearities of the microelectrode For current clamp recordings the amplifier is equipped with a BRIDGE balance circuit which can fully compensa...

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