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P a g e
10.
Focusing on the specimen
10.1
Focus on the specimen
Focus on the specimen using the 10x objective. To avoid
touching the specimen during focusing, you should raise the
stage to its maximum height adjustment and observe
whether it is close to the objective at first, then using the
fine focus slowly lower the stage while observing through
the eyepieces until a clear crisp image is seen.
10.2
Condenser adjustment
Turn the condenser focusing knob to move the condenser up or
down. Raise the condenser when using the high magnification
objectives, and conversely lower it when using a lower
magnification objective.
•
The condenser and objective are coaxial. The
condenser has been adjusted and pre-set before leaving the
factory, and the maximum height has been pre-set, so the end-
user has no need to adjust it oneself.
10.3
Aperture Iris Diaphragm Adjustment
Turn the aperture iris diaphragm lever to adjust the aperture iris
diaphragm.
•
The aperture iris diaphragm is designed for the
adjustment of the numerical aperture, not for
brightness.
•
Generally, setting the aperture iris diaphragm to 70 % –
80 % of the numerical aperture (N.A.) of the objective
in use will provide an image with good contrast. If one
chooses to observe the image of the aperture iris
diaphragm, one can remove one eyepiece and look
down the eyepiece tube. One will observe a dark ring
encroaching from the sides of the tube at the end of the
viewable range.
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