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NICOYA LIFESCIENCES

1-CHANNEL OPERATION MANUAL V4.1.01

Released: January 18, 2018 (V4.1.01)

Содержание OpenSPR

Страница 1: ...NICOYA LIFESCIENCES 1 CHANNEL OPERATION MANUAL V4 1 01 Released January 18 2018 V4 1 01 support nicoyalife com...

Страница 2: ...re Setup 12 1 3 1 Installing TraceDrawer 12 1 3 2 TraceDrawer License Activation 12 2 STANDARD OPERATING PROCEDURES 14 2 1 Preparation for your Experiment 14 2 1 1 Required Items 14 2 1 2 Fluidic Setu...

Страница 3: ...Controls 48 2 6 Shutdown Procedures 49 2 6 1 Ending a Test 49 2 6 2 Standby Mode 50 2 6 3 Instrument Shutdown 51 2 7 Data Output Files 54 3 MAINTENANCE AND CLEANING 55 3 1 Preparation of Reagents 55 3...

Страница 4: ...he safety of the materials being used in the system before proceeding and utilize all appropriate and recommended safety precautions associated with the materials The sample holder docking system cont...

Страница 5: ...te in the system after completing tests and that the unit is stored with a closed lid 10 Handle Sensor Chips with tweezers and while wearing gloves Figure 0 1 1 and other appropriate personal protecti...

Страница 6: ...injection port and replacement will be at the user s expense This information is based on our present knowledge However this does not constitute a guarantee for any specific product features and shal...

Страница 7: ...x Surge protected power outlets are recommended 1 1 2 Standard Equipment Supplies The OpenSPR Starter Pack will come equipped with the following supplies Table 1 OpenSPR Starter Pack supplies Quantity...

Страница 8: ...250 mL glass bottle that does not contain the tube fitting without the black attachment on top This will be your Waste Bottle tip it is good practice to label this bottle accordingly Find the 3 outlet...

Страница 9: ...ube is in the final position gently tighten the fitting clockwise until the tube is secured into place do not over tighten Figure 1 2 Left Loosen the fitting by turning it counter clockwise before ins...

Страница 10: ...e OpenSPR instrument Figure 1 4 and connect to the recommended power outlet Figure 1 4 USB and power cable ports on the back of the OpenSPR Plug one end of the USB Cable into the back of the OpenSPR F...

Страница 11: ...x will contain the installer for both the OpenSPR and TraceDrawer software 1 2 2 Installing OpenSPR Software 1 Ensure that the computer meets the specifications listed in Section 1 2 1 It is highly re...

Страница 12: ...the software must be restarted 1 3 TraceDrawer Software Setup Setup of the TraceDrawer analysis software is only applicable if a TraceDrawer license key was purchased 1 3 1 Installing TraceDrawer Copy...

Страница 13: ...will restart with your license key activated The TraceDrawer software will now be ready to use and will display the home screen Figure 1 7 For details on how to perform data analysis using TraceDrawe...

Страница 14: ...Relevant Personal Protective Equipment 2 1 2 Fluidic Setup Prior to starting your experiment the fluidic setup must be in place for proper function of the OpenSPR This includes having the Waste Bottle...

Страница 15: ...Place the bottle onto the tray of the OpenSPR next to the Waste Bottle Figure 2 2 Buffer Bottle 1 and 2 250 mL square glass bottles containing a tube fitting Prepare at least 100 250 mL of each runnin...

Страница 16: ...accumulation It is recommended to keep at least 50 mL of each running buffer in a separate container to use to rinse the injection port during your experiment 2 1 3 Optical Setup The 1 Channel OpenSPR...

Страница 17: ...wer source Power on the OpenSPR using the Power Button on the front of the instrument Figure 2 4 The blue lights of the OpenSPR should turn on and the instrument will initialize Figure 2 4 Location of...

Страница 18: ...estart the software Tip to power off the OpenSPR hold the power button down for 10 seconds until the power LED turns off Click Start to enter the software The software will walk you through the Instru...

Страница 19: ...section Figure 2 8 Figure 2 8 Edit button located in the Fluidic Priming section This allows the user to input the different buffer solutions used in the test Clicking this icon will open a new window...

Страница 20: ...is being used for the first time after a full shutdown 3 If you would like to use a new running buffer for your test 4 If you are going to be starting with a running buffer in an alternate bottle posi...

Страница 21: ...gure 2 11 Instructions for placing a chip into the system can be found in Section 2 4 1 Do not install the sensor to be used for your experiment at this stage to perform the priming When a chip is doc...

Страница 22: ...he priming select Next Figure 2 13 Confirmation to begin instrument priming 4 A window with a progress bar will appear indicating how much time is remaining in the process Figure 2 14 During the primi...

Страница 23: ...quired as well as expected reference for 1 Channel OpenSPR It is only recommended to take new references in the below scenarios 1 The previous references were acquired more than 1 month ago 2 An insuf...

Страница 24: ...e the references a clean blank chip must be installed in the OpenSPR Figure 2 17 To install a blank chip into the OpenSPR follow the directions below Figure 2 17 Optical reference setup instructions 2...

Страница 25: ...ing the blank chip into the sensor stage close the lid of the OpenSPR then dock the stage using the button available in the window 7 Once the blank chip is fully installed into the OpenSPR select Next...

Страница 26: ...gin taking the optical references The process will take approximately 2 minutes to complete and the progress will be displayed Figure 2 22 Keep the lid of the OpenSPR closed during this process Figure...

Страница 27: ...g you through the processes of loading a new sensor and removal and prevention of bubbles within the fluidic path Full details for each of these procedures are provided in the sections below Figure 2...

Страница 28: ...r is in the docked position the software will first ask if you would like to continue with the current sensor installed or if you would like to load a new one Figure 2 25 To place a new sensor into th...

Страница 29: ...n the face of the flow cell by gently wiping it with a lint free wipe dampened with isopropanol diluted isopropanol will work for this purpose as well After cleaning the flow cell allow it to air dry...

Страница 30: ...this will scratch the surface and severely diminish its performance Figure 2 29 Front of the sensor chips face the label stored in the container The front sensor surface can also be identified using...

Страница 31: ...Figure 2 31 Proper installing of a sensor chip into the sensor holder 9 Place the holder containing the sensor into the docking stage Ensure it is fully seated within the docking stage 10 Close the li...

Страница 32: ...per seal is not made between the sensor and flow cell If liquid is present outside of the flow cell select Back to begin the process to re load the sensor If no leaking is present select Next to proce...

Страница 33: ...To remove bubbles a solution of 80 isopropanol will be used The software will prompt you to load this solution into the injection port at least 150 L volume is recommended Figure 2 36 The solution sh...

Страница 34: ...still present click Repeat which will bring you back to the initial step where you will need to load more isopropanol into the injection port Repeat this process until all bubbles are removed Figure...

Страница 35: ...all data for the test is saved Note that the date and time of your test will be automatically be appended onto the name If a name is not given then the test will be saved simply under the date and ti...

Страница 36: ...e data will begin streaming into the graph Figure 2 42 The axes scales will start very small and will automatically grow to accommodate the data in both x and y as data is collected Approximately 4 po...

Страница 37: ...ss to change the running buffer The user will be prompted to select the new running buffer to change to Figure 2 44 Figure 2 44 Buffer change dialogue prompt Prior to commencing the automated buffer c...

Страница 38: ...d near the end of the buffer change process when a separate fluidic line in the OpenSPR is flushed Figure 2 46 Example response graph of a buffer change to a higher refractive index running buffer Onc...

Страница 39: ...controlled via the software This section will outline the full injection process Syringes and tips User samples and solutions are introduced into the OpenSPR using syringes containing blunt end injec...

Страница 40: ...on the size of sample loop installed in the OpenSPR The minimum sample volume required is the volume of the sample loop 50 L Excess volume in addition to that of the sample loop is required to ensure...

Страница 41: ...edle into the injection port and push the air through the sample loop The excess buffer and eventually will exit via the outlet line followed by air 3 Load a syringe with the sample solution to inject...

Страница 42: ...ur sample over the sensor The time remaining in the injection is shown above the menu bar 5 When an injection is made an Injection Details box will appear Figure 2 52 In this box the user can input th...

Страница 43: ...hich walks the user though the recommended procedures for immobilization of a ligand onto the various sensor chemistries available The ligand immobilization wizard can be accessed by clicking the Liga...

Страница 44: ...lization target to obtain sufficient analyte signal for analysis Figure 2 56 Enter in the molecular weights of your biomolecules into the ligand wizard After this step the specific protocols for each...

Страница 45: ...d but the time can be adjusted from 1200 30 s Figure 2 58 Ligand immobilization injection stage Upon performing the ligand injection the wizard will automatically move forward onto the evaluation stag...

Страница 46: ...the user can zero the graph as well as open advanced settings to manually adjust the axes scale Figure 2 60 Graph controls in menu bar Zeroing the Graph Clicking Zero in the graph menu Figure 2 61 wi...

Страница 47: ...cales back to the default values to encompass all of the data displayed in the graph Selecting Set Custom Axes will open a menu which will allow you to manually set the minimum and maximum values of b...

Страница 48: ...s On the right hand side of the menu bar there is a bar which allows you to open advanced instrument controls Figure 2 64 Normally during a test the user should not have to use these but they do give...

Страница 49: ...nd will restart the data acquisition on the Experiment Data screen This option is great if you want to perform a quick data analysis to evaluate the results collected while still being able to continu...

Страница 50: ...would like to continue using the same sensor for analysis in subsequent tests it is recommended to leave the sensor installed in the OpenSPR during standby mode If the sensor is no longer going to be...

Страница 51: ...home screen The status of the instrument will be displayed and the instrument can be left unattended Important Do not shut down the software or the OpenSPR instrument while in standby mode 2 6 3 Inst...

Страница 52: ...ure 2 71 To do this place all 3 inlet tubes into a bottle containing filtered deionized water multiple bottles can be used as necessary so long as all inlet tubes are submerged Once all inlet tubes ar...

Страница 53: ...inlet tubes from solution and suspend the ends of the tubes in a clean safe space Once all inlet tubes are in proper position click Next to begin the purging step Figure 2 73 Shutdown process 3 3 purg...

Страница 54: ...d in both the X and Y scales Inside this folder you will find several files which are detailed below File Details Test Date Time Filename_CH1 Response data for each injection normalized for kinetic an...

Страница 55: ...mode will maintain a low flow of running buffer DI water through the fluidic lines to prevent any buildup of salts sediment The OpenSPR software contains built in procedures to place the instrument i...

Страница 56: ...t 20 mL of DI water is present in a separate accessible container Empty the Waste Bottle before commencing 2 Begin the prime sequence and load a blank into the OpenSPR sample holder Continue to prime...

Страница 57: ...ls Required 2 mL sodium dodecyl sulphate SDS 0 5 2 mL Urea 6 M 2 mL Sodium Bicarbonate 0 2 M 2 mL Acetic Acid 1 2 mL Sodium Hypochlorite 0 5 DI Water filtered Blank Chip 6x disposable syringes with bl...

Страница 58: ...enSPR perform a bubble removal injection of this solution 9 Once the bubble removal sequence with the 0 2 M sodium bicarbonate is complete rinse the injection port with 3 mL of DI water 10 Rinse the i...

Страница 59: ...ment the user will interface with the power button and injection port Figure 4 2 The injection port is used to insert the user sample into the instrument using a syringe containing a blunt end injecti...

Страница 60: ...s in the OpenSPR interfaces with the sensor through the flow cell The flow cell is composed of a PDMS material containing 2 microfluidic channels The sensor chip will seal against the flow cell allowi...

Страница 61: ...61 OpenSPR 1 Channel Operation Manual V4 1 01...

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