13
•
If the objective phase plate and the annular of the slider do not coincide, use the two hexagonal
screwdrivers supplied with the microscope to bring the slider annular ring to the center of the
phase plate, so that the image of the annular diaphragm is concentric with the phase plate image.
•
If the slider annular ring image is moved from the phase plate image in the objective, a low
phase contrast image will result.
•
For phase contrast microscopy at the maximum contrast, use GIF (Green interference filter)
in the optical path.
•
Place the filter in the designated retaining ring above the phase annular diaphragm slider.
Filter selection
Filter type
Procedure
GIF (Green interference) 546nm
For phase contrast and contrast adjustment with black
and white film
NCB (Neutral Color Balance)
Blue
For general microscopy and color photomicrography
VI Photo Procedure
•
The optical path selector lever can be used to set the optical path to either the Binocular tube 100:0 or
Binocular tube/vertical tube 20:80 (observation: photo).
•
Before starting, make sure all prior steps in this manual are followed.
•
In order to attach a camera to the microscope, an additional camera adapter must be purchased.
See picture below for reference. Also refer to the parts and accessories section on page 17.
