Molecular Devices SpectraMax M2, Руководство пользователя

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Chapter 4: Operating the Instrument
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Fluorophore Excitation Wavelength (nm) Emission Wavelength (nm)
HPPA 320 405
4-MeU, NADH, NADPH 355 460
Biotinidinase 355 544
PKU 390 485
Green Fluorescent
Protein 390 510
Attophos /Attofluor 444 555
FITC 485 538
Ethidium Homodimer
(DNA) 530 620
TRITC, Ethidium Bromide 544 590
Texas Red 584 612
TAMRA 547 580
Tryptophan 280 340
Fluorescence Fluorophores Wavelengths
Read a Plate
CAUTION! Before you read standard 96-well or 384-well plates from the top, you must
insert the plate adapter into the plate drawer. Before you do a bottom read, or when
you use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the
plate adapter from the plate drawer. See
Plate Adapter on page 19 .
Before you place a plate in the drawer, the underside of the plate must be dry. If the plate
has fluid on the underside, you must dry it with a lint-free cloth before you place it in the
drawer.
1. On the rear of the instrument, turn on the power switch.
The instrument carries out a diagnostic check. During this check, the plate drawer opens,
and then closes. When the diagnostic check completes, the plate drawer opens and
remains open.
2. Power on the computer.
3. Start the SoftMax Pro Software and connect the software to the instrument.
4. In the software, in the Ribbon, on the Home tab, click Acquisition Settings to display
the Settings dialog where you define the acquisition settings for the Plate section.