SpectraMax L Luminescence Microplate Reader User Guide
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0112-0174 C
Change Aperture
- Displays a message when the instrument is ready for you to
change the plate aperture from 96-well plates to 384-well plates or vice-versa. The
instrument configuration has either a manual aperture or an automatic aperture. For
the manual aperture configuration you must use the Change Aperture command
when you switch plate formats. See
Changing the Plate Aperture on page 21
Shake Settings
- Displays the Shake Plate Settings dialog. The Shake Plate Settings
dialog enables you to custom define how to shake a plate. This shake process is
independent of a protocol. When you create a protocol, you can define shake
parameters on the Settings dialog.
Note:
The Shake Settings icon on the Operations tab is independent of the Shake
icon on the Home tab.
Instrument Calibration
Calibrate the instrument once a year for maximum performance. In the SoftMax Pro
Software, select the Operations tab and click Calibration to display the Calibration dialog.
There are two calibration methods:
Select
Internal Light Emitting Diodes
to calibrate using the instrument’s onboard
LEDs. This method does not require sample preparation.
Select
Custom Multi-PMT Normalization Microplate
when the instrument has
multiple PMTs and you want to use the light spectrum from your own assays to
calibrate the instrument, rather than the light spectrum from the factory configured
LEDs.
Calibrate with Internal Light Emitting Diodes
To calibrate using the internal light emitting diodes:
1. Remove any plate from the plate drawer.
2. In the SoftMax Pro Software, select the Operations tab and click
Calibration
to
display the Calibration dialog.
3. Select the
Internal Light Emitting Diodes
Calibration Method option.
4. Click
OK
. Calibration is done automatically.
Custom Multi-PMT Normalization Microplate
Create your own custom calibrations if you prefer to calibrate using the light spectrum
from your assays rather than the light spectrum from the factory configured LEDs.
Custom calibrations are used from the Settings dialog in the Sensitivity category. See
There are two different PMT Calibration Factor options:
Select
Auto Calculation
to use your own assay materials for calibration and you want
the software to automatically calculate a PMT calibration factor.
Select
Manual Set
to use your own assay materials for calibration and you want to
manually calculate the PMT calibration factor based on the signal obtained from each
PMT.
