Molecular Devices SkanWasher 300, Руководство пользователя

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Troubleshooting
SkanWasher 300 User Guide 45
Troubleshooting Tips
Problem Things to Try
Cells are aspirated
off the bottom of the
wells. Decrease vacuum pressure at pump by
increasing value. Optimize cell assay with
vacuum pressure of 350-450 mbar.
Cells are being disrupted during dispens-
ing, lower air pressure by decreasing air
pressure value in software through
Edit
Program .
Check Aspirate position in Edit Program
from the Setup Menu in the software. Re-
set height of aspiration probes. For low
residual volume, the aspirate probe must
be close to the bottom of the well.
Check Dispense position in Edit Program
from the Setup Menu in the software. Re-
set height of dispense probes.
Residual volume too
high or low. Check vacuum pressure. Pressure setting
of 250 mbar is suggested for ELISA appli-
cations. Increasing the value decreases the
vacuum pressure, which slows the aspira-
tion rate. Use a slower aspiration rate when
working with cells.
Check Aspirate position in Edit Program
from the Setup Menu in the software. Re-
set height of aspiration probes. For low
residual volume, the aspirate probe must
be close to the bottom of the well.
If residual volume too low, check also for
clogged probes. Is volume low in certain
wells?