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Chapter 1: ImageXpress Pico Automated Cell Imaging System

5063666 B

17

Theory of Operation

The ImageXpress Pico System uses the following components and functions:

Fluorescence Imaging, see page 18

Excitation and Emission Filters, see page 18

Dichroic Mirror, see page 19

Objective Lenses, see page 20

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Содержание ImageXpress Pico

Страница 1: ...5063666 B June 2018 ImageXpress Pico Automated Cell Imaging System Installation Guide...

Страница 2: ...ght and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical info...

Страница 3: ...ellReporterXpress Software Features 16 Theory of Operation 17 Obtaining Support 20 Product Documentation 22 About This Guide 22 Chapter 2 Setting Up the ImageXpress Pico System 23 Package Contents 23...

Страница 4: ...A Instrument Specifications 41 Instrument Dimensions 43 Appendix B Compatible Objectives 47 Appendix C Filter Cube Specifications 49 Appendix D Replacement Parts and Optional Extras 51 Appendix E Pac...

Страница 5: ...of other types of hazards such as biohazard electrical or laser safety warnings as are described in the text of the warning When warnings and cautions are displayed in this guide be careful to follow...

Страница 6: ...Molecular Devices instruments Symbol Indication Indicates that the product documentation must be consulted Indicates a potential lifting hazard For information about the weight of the instrument Indic...

Страница 7: ...dicates that there is an authorized representative in the European community Indicates the instrument manufacturer Indicates the manufacturer catalog number Before Operating the Instrument Make sure t...

Страница 8: ...he instrument and the wall Power off the instrument when not in use If the instrument does not power on you may need to replace the instrument fuses See Replacing Fuses on page 8 for details Replacing...

Страница 9: ...ower button to power on the instrument Note If the instrument still does not power on after you change the fuses contact Molecular Devices Technical Support See Obtaining Support on page 20 for detail...

Страница 10: ...s to prevent the spread of disease Observe all cautionary information printed on the original containers of solutions before their use Dispose of all waste solutions based on the waste disposal proced...

Страница 11: ...her maintenance tasks must be done by qualified Molecular Devices personnel only Contact a Molecular Devices service engineer to inspect and perform a preventive maintenance service on the instrument...

Страница 12: ...ImageXpress Pico Personal Imaging System Installation Guide 12 5063666 B...

Страница 13: ...eatures powerful image analysis capabilities and over 25 available predefined experimental protocols the ImageXpress Pico System becomes an extremely flexible and programmable device ideally suited fo...

Страница 14: ...tures a resolution of better than 0 25 m The ImageXpress Pico System uses Fluotar objectives manufactured by Leica Microsystems The selected objective lens focuses excitation light onto the sample and...

Страница 15: ...image quality depends on plate flatness well bottom thickness and optical clarity A spring loaded mechanical clamp holds the sample plate securely in the plate holder The clamp automatically opens wh...

Страница 16: ...ystem that streamlines automated imaging to offer a simplified solution for scaling up microscopy Its features include A web based interface that runs on many browsers including those found on iPads a...

Страница 17: ...g System 5063666 B 17 Theory of Operation The ImageXpress Pico System uses the following components and functions Fluorescence Imaging see page 18 Excitation and Emission Filters see page 18 Dichroic...

Страница 18: ...molecule and their specific properties determine the initial selection of the optical components to be used such as the emission and excitation filters and the dichroic mirror Excitation and Emission...

Страница 19: ...e emission filter preventing it from entering the imaging system of tube lens and camera The optics in a filter cube are interference filters made by depositing thin film coatings on a glass support T...

Страница 20: ...that you are imaging The physical thickness can be determined by the plate specifications from the plate manufacturer See Compatible Objectives on page 47 for details Obtaining Support Molecular Devic...

Страница 21: ...roscopy courses www leica microsystems com science lab www ibiology org ibioeducation taking courses ibiology microscopy short course html The Molecular Probes Handbook offers advice on fluorescent pr...

Страница 22: ...e to view Help for the current page Tip Molecular Devices recommends that you review the documentation before installing or using the ImageXpress Pico System or the CellReporterXpress Software About T...

Страница 23: ...package includes the following Item Description Installation Guide Two 2 CAT6 Ethernet cables 2 m 6 56 ft Power cord 115 V 1 m 3 3 ft or Power cord 230 V 1 m 3 3 ft Accessory Pack Stage Insert contai...

Страница 24: ...revent injury use a minimum of two people to lift the instrument CAUTION Retain the shipping box and all packaging materials for future transport needs CAUTION When transporting the instrument warrant...

Страница 25: ...emove the plastic bag from the instrument 6 With one person on each end lift the instrument to a dry flat surface CAUTION Lift the instrument from the bottom avoid lifting from the top cover Keep the...

Страница 26: ...ources of vibration such as a centrifuge During installation you will need to access the back of the instrument to connect the cables After installation allow at least 20 cm 7 9 in clearance on all si...

Страница 27: ...onnected to your network other computers on the network can connect to the host computer as remote clients You can also distribute functionality across several computers on the network using more powe...

Страница 28: ...grounded electrical wall outlet Network Configuration or Server Configuration To connect cables for a network configuration or server configuration 1 Do the following to connect the supplied Ethernet...

Страница 29: ...ent is connected to a network connect the Ethernet to the LAN2 port on the back of the instrument CAUTION The network connections between the host computer imaging device remote clients and external c...

Страница 30: ...ImageXpress Pico Personal Imaging System Installation Guide 30 5063666 B...

Страница 31: ...for the ImageXpress Pico System You will use the CellReporterXpress Software to work with the ImageXpress Pico System and control its functions See the CellReporterXpress Installation Guide for detail...

Страница 32: ...ImageXpress Pico Personal Imaging System Installation Guide 32 5063666 B...

Страница 33: ...e Login field enter the Windows system user name 3 In the Password field enter the Windows system password 4 Click LOG IN Note The CellReporterXpress Software uses the Windows login credentials of the...

Страница 34: ...tasks Ensure that all components and access doors are closed before starting the instrument In addition observe the following when handling a filter cube CAUTION To prevent skin oils from damaging th...

Страница 35: ...e 5 Click the Choose Filter dropdown and select the filter cube you want to install 6 Click Open Maintenance Door 7 If needed slightly rotate the filter cube turret by hand to get direct access to the...

Страница 36: ...n objective CAUTION To prevent skin oils from damaging the optical coatings Molecular Devices recommends that you wear powder free disposable gloves when handling objectives and filter cubes CAUTION W...

Страница 37: ...want to install click Component Exchange 5 Click the Choose Objective dropdown and select the objective you want to install 6 Click Open Maintenance Door 7 Install the objective in the slot by gently...

Страница 38: ...on Guide 38 5063666 B Inserting the Plate Holder or Slide Holder Open the sample door on the top of the instrument to insert the plate holder or slide holder in the sample X Y stage The sample X Y sta...

Страница 39: ...op right of the instrument to open the sample door 2 With the A1 label at the top left and the spring loaded clamp in the open position as shown insert the plate holder in the sample X Y stage Note Us...

Страница 40: ...te its top left corner To insert the slide holder 1 Press the button at the top right of the instrument to open the sample door 2 With the A1 label at the top left as shown insert the slide holder in...

Страница 41: ...osition the instrument so that it is difficult to operate the power switch on the front Item Description Operating Environment Indoor use only System Power 100 VAC to 240 VAC 50 60 Hz 1 6 A nominal at...

Страница 42: ...6 C 11 F above ambient up to 40 C 104 F Ambient Operating Conditions Temperature 18 C to 30 C 59 F to 86 F Temperature recommended when using temperature control 20 C to 24 C 68 F to 75 F Relative Hum...

Страница 43: ...Appendix A Instrument Specifications 5063666 B 43 Instrument Dimensions Front View Item Description 1 67 8 cm 26 69 in 2 35 3 cm 13 90 in 3 55 1 cm 21 68 in...

Страница 44: ...ImageXpress Pico Personal Imaging System Installation Guide 44 5063666 B Side View Item Description 1 45 3 cm 17 83 in 2 39 7 cm 15 63 in...

Страница 45: ...Appendix A Instrument Specifications 5063666 B 45 Top View Item Description 1 42 5 cm 16 73 in 2 19 5 cm 7 68 in...

Страница 46: ...ImageXpress Pico Personal Imaging System Installation Guide 46 5063666 B...

Страница 47: ...l Aperture NA Working Distance Correction Collar PL FLUOTAR 4x 0 13 4x 0 13 17 0 mm No HC PL FLUOTAR 10x 0 32 10x 0 32 11 1 mm No HC PL FLUOTAR 20x 0 40 20x 0 4 7 4 mm No HC PL FLUOTAR L 40x 0 60 CORR...

Страница 48: ...ImageXpress Pico Personal Imaging System Installation Guide 48 5063666 B...

Страница 49: ...ces are compatible with the ImageXpress Pico System Filter Wavelengths DAPI Excitation 370 40 nm Emission 450 60 nm Dichroic 410 nm FITC Excitation 465 40 nm Emission 525 30 nm Dichroic 500 nm TRITC E...

Страница 50: ...ImageXpress Pico Personal Imaging System Installation Guide 50 5063666 B...

Страница 51: ...endix D Replacement Parts and Optional Extras For an up to date list of replacement parts and optional extras go to www moleculardevices com See Compatible Objectives on page 47 for a list of compatib...

Страница 52: ...ImageXpress Pico Personal Imaging System Installation Guide 52 5063666 B...

Страница 53: ...holder or the slide holder from the sample X Y stage 2 Remove any installed filter cubes and pack them in their original packaging See Installing a Filter Cube on page 34 for details on removing a fil...

Страница 54: ...bottom foam packing as shown below WARNING LIFTING HAZARD To prevent injury use a minimum of two people to lift the instrument CAUTION Keep the instrument upright and level when lifting Do not tip or...

Страница 55: ...ort 5063666 B 55 11 Seal the box for transportation CAUTION Keep the box upright during transport Do not tip or tilt the box or place it on its side See Unpacking the Instrument on page 24for details...

Страница 56: ...ImageXpress Pico Personal Imaging System Installation Guide 56 5063666 B...

Страница 57: ...C Notice This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 18 of the FCC Rules These limits are designed to provide reasonable protection...

Страница 58: ...ty of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC P...

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