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Chapter 1: ImageXpress Pico Automated Cell Imaging
System
The ImageXpress® Pico Automated Cell Imaging System by Molecular Devices® is an
affordable all-in-one platform for automatically acquiring and analyzing images from
fluorescently labeled biological samples in plates and slides. It enables you to increase the
throughput of your image acquisition and analysis, allowing you to gain insights in minutes.
The core hardware component of the imaging system is a custom-designed, fully automated,
epi-illumination fluorescence microscope. The rapid autofocus and precision sample
movement features of the microscope allow large numbers of high-resolution images to be
acquired in the shortest possible time. All key optical and mechanical elements are
motorized, with asynchronous command execution, allowing complete control of the
instrument configuration.
When used in combination with the CellReporterXpress Image Acquisition and Analysis
Software, which features powerful image analysis capabilities and over 25 available
predefined experimental protocols, the ImageXpress Pico System becomes an extremely
flexible and programmable device, ideally suited for user-defined, high-speed automated
assays.
Key components of the instrument include the following:
Built-in, internal light source comprised of four high-powered LEDs enables very high
sensitivity fluorescent imaging. Additional LED-based light sources allow transmitted light
and planetary view imaging.
High-sensitivity, 5-megapixel CMOS camera.
Hardware-based autofocus system with precision motorized Z-stage focus.
High-transmission fluorescence imaging optics with world-class chromatic aberration
correction, resolution, and image flatness.
Planetary view camera provides overview imaging for slides.
Precision motorized sample (X-Y) stage.
High-quality Leica objectives in a six-position turret.
Filter cubes in a six-position turret.
Motorized selection of stage position, filter cubes, and objectives.
Temperature control for live cell imaging at 6°C (11°F) above ambient up to 40°C (104° F).
Operation and configuration control by the integrated CellReporterXpress Software.
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