Transmitted Light Option Operations
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4.
Move the field-stop slider, located just above the
A
label on the
condenser turret, all the way to the right.
Figure 4-17:
Field stop slider
5.
Select the diffusion filter, or place some lens paper or lint-free
wipes on top of the condenser lens to serve as a diffuser. If
necessary, weigh them down slightly to keep them from blowing
away.
6.
In the MetaXpress software, select the desired phase contrast
objective.
7.
Set the focus (Z position) to approximately 8000
μ
m.
If necessary, you can adjust this later.
8.
Select the Transmitted Light illumination setting.
9.
With no sample present, snap an image.
10.
If necessary, adjust the illuminator level and the exposure time
to obtain a visible but not saturated image. The image of the
phase ring might be off-center. See
acceptable as long as most of the image is visible.
ImageXpressOptionsUserGuide.book Page 69 Wednesday, September 9, 2015 3:54 PM
