Molecular Devices FLIPR Tetra, Руководство пользователя

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FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 193
Fluid Transfer Settings—Dispense
Recommended Assay Settings
Post Assay Cell Suspension Cleaning
1. The suspension cell system should NEVER be left without
cleaning at the end of an assay day or between assays with
different cells.
2. Remove cell spinner flask and insert tubing from flask into DI
water solution.
3. Insert return line tubing to a waste flask making sure that the
tubing does not reach into the waste fluid. It will need to be left
as clean as the inlet tubing.
4. Perform a manual reservoir wash with water and follow by
wiping out the reservoir with a Kimwipe.
5. Build a FLIPR
®
Tetra System protocol to wash the reservoir and
all tubing in the following order:

5 times with Endotoxin-free DI water.

5 times with Cytoclense Sterilant wash and soak (2 min).

5 times with Endotoxin-free DI water.
Parameter Suspension Setting Adherent Settings
Target Plate Read Plate Read Plate
Volume 25 μ L 25 μ L
Height 15 μ L 30 μ L
Speed 30 μ L/s 20 μ L/s
Removal Speed 10 mm/s 10 mm/s
Expel Volume
1
1. A hold or expel volume will introduce air bubbles into the well causing false
spikes in signal.
0 μ L 0 μ L
Pause in Well None None
Hold pipettor during
dispense
None None
Parameter Setting
Reduction Type Area under the curve, from or shortly after addition
point to the end of the assay.
Correction Subtract bias based on Sample 1.