Description
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Spectrum Reads
Spectral analysis measures across a spectrum of wavelengths (excitation
250 nm to 850 nm, emission 360 nm to 850 nm). When reading using a
specific detection mode, such as fluorescence, you can set a fixed
wavelength for excitation and scan the emission wavelengths, or set a
fixed wavelength for emission and scan the excitation wavelengths. All
spectrum readings are made using scanning monochromators.
In Spectrum reads, the fluidics module is not enabled.
For more information on this read type, please review the appropriate
section in the SoftMax Pro user guide.
Well Scan Reads
Some applications that involve the detection of whole cells in large area
tissue culture plates can require the use of Well Scan reads. As many cell
lines tend to grow in aggregates or in the edges of microplate wells, this
non-confluent growth pattern can require multiple reads at different
locations in a well.
When used with 6-well, 12-well, 24-well, 48-well, or 96-well plates, well
scanning allows maximum surface area detection for whole cell assays.
No plate adapter is used for tissue culture plates of 24 wells or less.
In Well Scan reads, the fluidics module is not enabled.
For more information on this read type, please review the appropriate
section in the SoftMax Pro user guide.
SoftMax Pro Software
The Molecular Devices SoftMax Pro Software is a highly integrated
program that can be used to:
•
Control the reader
•
Collect data
•
Analyze data
SoftMax Pro software is easy to use, yet is powerful and flexible, and is
necessary to access the full capabilities of the FlexStation 3 instrument.
SoftMax Pro Software allows you to:
•
Set Up the Reader and Software Parameters, see page 42
•
Acquire Data from the Reader, see page 43
•
Perform Complex Data Analysis, see page 44
FlexStation_manual.book Page 41 Tuesday, June 25, 2013 10:22 AM
