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Glossary
Absorbance
Absorbance is the amount of light absorbed by a solution. To measure
absorbance accurately, it is necessary to eliminate light scatter. In the
absence of turbidity, absorbance = optical density.
A = log
10
(I
0
/I) = –log
10
(I/I
0
)
where
I
0
is incident light before it enters the sample,
I
is the intensity of
light after it passes through the sample, and
A
is the measured
absorbance.
Automix
The Automix function in the SoftMax Pro Software determines if and how
often automated shaking of the microplate is performed during a reading.
Emission Cutoff Filter
An emission cutoff filter is a long pass filter used to condition the emission
light before detection by the PMT. In automatic mode, the instrument
sets the cutoffs automatically based on the wavelengths chosen for
reading. In manual mode, you can choose the filter wavelength.
Emission Spectrum Scan
An emission spectrum scan measures fluorescence or luminescence
across a spectrum of wavelengths. Fluorescent reads use the emitted
light from a fixed excitation wavelength. The default value reported for
each well is the wavelength of maximum emission in either RFU for
fluorescence mode or RLU for luminescence mode.
Endpoint
In an Endpoint read, a reading of each microplate well is taken in the
center of each well, at a single wavelength or at multiple wavelengths.
Depending on the read mode, raw data values are reported as optical
density (OD), %Transmittance (%T), relative fluorescence units (RFU), or
relative luminescence units (RLU).
FlexStation_manual.book Page 163 Tuesday, June 25, 2013 10:22 AM
