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00110230w  Rev 10/20 

13 of 24

TIP

The continue-arrow in the lower right 

screen indicates more menu options 

V

Counting Process

Perform 

Attach the Sensor, p .11

 

before continuing with this section.

1.  Immediately after mixing, submerge 

the tip of the sensor into the middle of 

the sample tube solution and press the 

OK button to begin counting. Resting 

the sensor against the tube bottom may 

result in inaccurate results. It is normal 

to hear the pump clicking. 

 

01-Feb-18 14:16

Counting Process

Ready

Submerge 

the Sensor . . .

then press OK

60µm Sensor

            

01-Feb-18 14:16

Counting Process

Loading Sample . . .

Keep the Sensor 

submerged

60µm Sensor

   

01-Feb-18 14:16

Counting Process

Counting . . .

Lift the Sensor 

out of the Sample

60µm Sensor

          

Note: 

It is important to press the 

OK button only after the sensor is 

submerged, and keep it submerged as 

long as the screen displays 

Keep Sensor 

submerged

. Do not to rest your thumb  

on the EJECT button during sampling,  

as it can cause inaccurate results.

01-Feb-18 14:16

Counting Process

Calculating . . .

Count

0

900

36

24 30

18

12

6

Dia . in µm

60µm Sensor

   

01-Feb-18 14:16

Counting Process

Saving . . .

Count

0

900

36

24 30

18

12

6

Dia . in µm

60µm Sensor

   

01-Feb-18 14:16

Counting Process

Eject Sensor to continue

Count

0

900

36

24 30

18

12

6

Dia . in µm

Count Complete

   

             

 2.   Scepter™ 3.0 will beep when sample loading is completed and the sensor can be lifted out of the solution. 

Pause briefly, while Scepter™ 3.0 creates the histogram. Press the EJECT button to eject the sensor into 

an appropriate cast off container when directed to do so on the screen. Discard used sensors appropriately. 

Scepter™ 3.0 Sensors are not reuseable. 

3.  When the count is complete, the histogram is automatically created. The concentration and diameter  

or volume will be displayed on the screen. Press the OK button to save (up to 999 histograms in the  

Scepter™ 3.0 memory), and open the 

Histogram Explorer . 

Press the OK button to return to Home screen.

Changing the histogram gating method and other features are done in 

Histogram Explorer

Note:

 Files are automatically named: 

ScepterID.Sample#.Date_time.png (or .csv)

.

 

Note

: When using Scepter™ 3.0 Test Beads, set gating to Auto for best results. 

Histogram Explorer

Up to 999 histograms can be saved for future review. When the display screen shows Disk Full, the acquired  

data can no longer be saved. To continue, histograms must either be deleted or uploaded to a computer.

Note: 

The counting procedure can still be performed when the disc is full, but histograms and data will  

not be saved. To delete histograms, refer to 

Deleting Saved Data, p .16

Histogram Explorer can be found  

two ways: 

From the Main Menu

, select 

Histograms, and the last saved or 

acquired histogram will be shown. 

Scroll to see the next histogram.  

Press the OK button to select.

01-Feb-18 14:16

Menu

Home

<

Histograms

Settings

Help

          

01-Feb-18 14:16

Histogram

Test 3  03-Dec-17

Count

0

900

36

24 30

18

12

6

Dia . in µm

3 .342e5

15 .682

/mL

µm

Conc .
Dia .

 

 

01-Feb-18 14:16

Histogram

Test 2  29-Nov-17

Count

0

900

36

24 30

18

12

6

Dia . in µm

3 .254e5

15 .785

/mL

µm

Conc .
Dia .

          

01-Feb-18 14:16

Histogram

Back

Go to Home 

Edit Analysis

Switch to Volume

Scale Y Axis Count

Export to USB

Print

<

After Counting Process

, the histogram will be displayed. 

Press the OK button to select. The Histogram Explorer Menu will appear. The blue “continue-arrow”  

is in the lower right corner, indicating additional option(s) can be viewed by scrolling down. 

Go to Home 

Prompts to start new sample count.

01-Feb-18 14:16

Histogram

Back

Go to Home 

Edit Analysis

Switch to Volume

Scale Y Axis Count

Export to USB

Print

<

          

01-Feb-18 14:16

Home 

Attach the Sensor 

to begin . . .

    

EJECT

((BEEP

))

CONTINUE-ARROW

Содержание PHCC30000

Страница 1: ...perates as MilliporeSigma in the U S and Canada The life science business of Merck KGaA Darmstadt Germany operates as MilliporeSigma in the US and Canada SigmaAldrich com User Guide Scepter 3 0 Handhe...

Страница 2: ...r computer easily No more cables The new Charging Station stores Scepter 3 0 out of the way when not in use freeing up valuable hood or lab bench space This system is intended for research use only Co...

Страница 3: ...nstrument to vibrations Vibrations may cause instrument malfunction or damage Do not autoclave or expose to high temperature To avoid damage to internal electronic components do not spray instrument w...

Страница 4: ...s the sensor Tech Service Port For Service use only USB Port Connects instrument to USB thumb drive Ergonomic Hook Allows comfortable handling of the instrument Secures the instrument to charger Contr...

Страница 5: ...the Recharging screen when placed in the Charging Station the following steps must be followed to start charging the Scepter 3 0 battery 1 Place Scepter 3 0 into the Charging Station 2 While on the s...

Страница 6: ...kable surface 1 Use double sided mounting tape 2 Remove tape liner from one side of tape and affix to back of mounting plate as shown below 3 Remove remaining tape liner and press firmly onto glass me...

Страница 7: ...14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 01 Feb 18 14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 1 Scroll to Edit Time and press the OK button 2 Scroll to...

Страница 8: ...nd select Properties If the File system is identified as FAT32 no formatting is needed Continue to Creating a Wi Fi Configuration File NOTE This view will also show how much space is available on your...

Страница 9: ...file from the computer onto the USB thumb drive Once the file has loaded remove the USB thumb drive from the computer port Connect to Wi Fi Network 1 From the Main Menu select Settings and press the...

Страница 10: ...were last set by the user either during a count or during post count data management 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Settings Back Date Time Bluetooth Wifi Gating M...

Страница 11: ...epter 3 0 instrument Do not use sensor if visibly damaged Attach the Sensor 1 When the Scepter 3 0 is powered on it will show Attach the Sensor to begin 2 Holding the sensor with the sensor size numbe...

Страница 12: ...net is recommend use low vacuum 5 Hg Cell Bead and Particle Suspensions Materials Required Cell sample Scepter 3 0 Test Beads Cat No PHCC3BEADS or particle suspensions Note Some particle suspensions...

Страница 13: ...ion and diameter or volume will be displayed on the screen Press the OK button to save up to 999 histograms in the Scepter 3 0 memory and open the Histogram Explorer Press the OK button to return to H...

Страница 14: ...03 Dec 17 Count 0 900 36 24 30 18 12 6 Dia in m 3 342e5 15 685 mL m Conc Dia 01 Feb 18 14 16 Histogram Edit Gates High gate Done 26 575 Low gate 12 585 Count 0 900 36 24 30 18 12 6 Dia in m Switch to...

Страница 15: ...wirelessly Materials Required Wireless Network broadcasting at 2 4 Ghz PC Mac or UNIX Computer connected to the same Wi Fi Network as Scepter 3 0 Scepter 3 0 Instrument Follow Wi Fi Network p 8 direct...

Страница 16: ...MS Press the OK button from any histogram on screen to open the Histogram Explorer menu 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Histogram Test 3 03 Dec 17 Count 0 900 36 24...

Страница 17: ...o printer Help Screens Tutorials 1 From the Menu screen scroll to Help press the OK button 2 Scroll to the topic of interest press the OK button 01 Feb 18 14 16 Scepter Software 4 6 01 Feb 18 14 16 Me...

Страница 18: ...ration of cell sample is within recommended operating range See Cell Bead and Particle Suspensions Test sample using Scepter 3 0 Test Beads Cat No PHCC3BEADS to ensure unit operating properly for conc...

Страница 19: ...y needs to be re charged Place Scepter 3 0 on charging stand to recharge the instrument See Battery p 5 Battery is not charging Ensure that the unit is correctly positioned in charging stand An animat...

Страница 20: ...ressing of OK button Fully submerge sensor in sample before pressing OK button Air bubble in sample Mix sample gently to avoid air bubbles Sensor lifted from sample before screen displays Sample Loade...

Страница 21: ...ed on FAT32 formatted USB thumb drive and try again See USB Thumb Drive Formatting p 8 Software update blocked on battery low Insufficient battery to perform update Recharge Scepter 3 0 and try again...

Страница 22: ...ntal Conditions Temperature 15 C to 25 C Maximum Relative Humidity 20 80 non condensing Storage Conditions Temperature 15 C to 30 C Maximum Relative Humidity 20 80 non condensing Scepter 3 0 Sensor Ma...

Страница 23: ...p E GP Sterile Vacuum Filtration System Millipore Express PLUS PES 0 22 500 38 12 SEGPU0538 0 22 500 45 12 SEGPU0545 0 22 1000 38 12 SEGPU1138 0 22 1000 45 12 SEGPU1145 Steritop E GP Sterile Funnel le...

Страница 24: ...Merck KGaA Darmstadt Germany and or its affiliates All Rights Reserved Global Registration Marks of Conformity USA TFB 1003 EUROPE C TAIWAN JAPAN R209 J00212 SOUTH KOREA R C Emd PHCC30000 RUSSIA BELAR...

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