Cellicon
TM
Perfusion Solution for Process Development User Guide
21
Symptom
Corrective Action
System leak
Check for cuts, cracks, or damage to assembly
Ensure system pressure is less than 5 psi
Ensure flowmeter clamp does not pinch the
tubing
Unexpected or
unstable pressure
readings
Ensure cable connections are good
Proper installation of P1 on Feed, P2 on
retentate, and P3 on permeate
Perform zero adjustment prior to run, after
priming
Clear air from the sensor
Unexpected
or unstable
flowmeter
readings
Ensure cable connections are good
Clean flowmeter and tubing with IPA
Close clamp around tubing and ensure
flowmeter clamp does not pinch the tubing
Ensure pump is running and flow is moving
Prime tubing in flowmeter, remove air bubbles
Choose correct calibration set in system settings
(tubing type, temperature)
Perform zero adjustment at the correct
temperature, prior to run and after priming
Re-zero sensor
Check flowmeter calibration against reference
flowmeter
Pump unable to
achieve target
flow rate
Re-prime tubing to remove air bubbles
Remove pump head from pump to clear air from
inside
Check for tubing pinch or kink
Check for filter fouling (high P1 with low P2)
Check dip-tube placement and ensure it does
not pull air from sparger
Check system mode, “flow control” vs “speed
control”
No flow through
permeate
Ensure permeate line is not clamped or pinched
Symptom
Corrective Action
Early filter fouling
(High P1 or low
P3)
Check that crossflow rate is at setpoint
Check system mode, “flow control” vs “speed
control”
Ensure maximum flux is within recommended
range
Swap out filter
Check flowmeter calibration setting is correct
Check flowmeter calibration against reference
flowmeter
System pressure
increase (High P1
and P2)
Check bioreactor pressure (ex. vent filter
clogging)
Check for tubing pinch or kink on retentate line
Check flowmeter calibration setting is correct
Check flowmeter calibration against reference
flowmeter
Cell growth issues
in bioreactor
Check flow rate and pump RPM are in
recommended range
Check flowmeter calibration setting is correct
Check for cell loss in permeate line
Prime filter during media hold only
Re-prime system to remove air bubbles
Check flowmeter calibration against reference
flowmeter
Cells found in
permeate
Swap out filter
Unexpected or
undesired alerts
Adjust alert settings
Adjust alarm limits
Perform factory reset
Follow recommendations provided for each alert
Data logging is
not saved
Ensure USB is inserted during run
Ensure USB drive has adequate space
USB is not removed until data logging is turned
off and software indicates it is safe to remove
