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Amaxa™ Nucleofector™ II Manual

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Содержание Amaxa Nucleofector II

Страница 1: ...Amaxa Nucleofector II Manual...

Страница 2: ...ture protocol updates also check www lonza com cell database For more information and future program updates check www lonza com nucleofection program update Nucleofector II CD ROM Nucleofector II Chi...

Страница 3: ...2 7 2 Selection of Nucleofector Programs by program code Free Program Choice 8 2 7 3 Selection of Nucleofector Programs by name of cell type Cell Type List 8 2 7 4 Selection and storage of Nucleofect...

Страница 4: ...2 3 0 Troubleshooting 12 4 0 Nucleofector II Device Error Codes 14 5 0 Appendix 16...

Страница 5: ...cations since the same transfection parameters apply for any nucleic acid substrate used DNA vectors e g expression plasmids or shRNA vectors and RNA e g siRNA duplexes can be transfected using our tr...

Страница 6: ...fferent cell lines and primary cells An up to date database of the continuously growing range of Amaxa Optimized Protocols can be found on our homepage at www lonza com cell database If no Amaxa Optim...

Страница 7: ...221 99199 0 or e mail ip cologne lonza com 2 2 Maintenance The Nucleofector II Device requires very little maintenance to assure reliable operation To clean and disinfect the case first unplug the po...

Страница 8: ...ainst great impact and vibration in moving and transporting the Nucleofector II Device Use the device with Lonza s certified Nucleofector Solutions and Lonza certified cuvettes only Use of any other s...

Страница 9: ...use 13 External electrode sockets For use within the Nucleofector 96 well Shuttle System 14 Receptacle for USB connection to Laptop 15 Receptacle for Serial connection to 96 well Shuttle The Nucleofec...

Страница 10: ...utton 1 Nucleofector II Device starts in the Free Program Choice mode Figure 3 If it does not start in the Free Program Choice press the functional button menu 5 repeatedly until Go to Free Program Ch...

Страница 11: ...he arisingwindowbypressingtheleftfunctionalbutton 5 Exit and save by pressing the X button 7 5 Saved custom programs can be selected by navigating throughthe CellTypeList usingthe Up and Down buttons...

Страница 12: ...ided through the settings To return from any sub menu to the main menu press the X button 7 2 8 2 Motor The cuvette carousel 8 is connected to a motor which enables automaticmovementmovemantofcuvettei...

Страница 13: ...he X button 7 3 The upgrade starts with a program table Confirm by pressing the X button 4 Secondly the list of optimized programs is loaded Confirm again with the X button 1 The stored custom program...

Страница 14: ...damage to the cells transfer cells immediately into pre warmed medium 37 C We recommend removing the sample from the cuvette directly after Nucleofection Avoid severe conditions during harvesting esp...

Страница 15: ...re low the plasmid amount can be increased up to 10 g per sample Increasing the DNA amount may lead to higher gene transfer efficiencies but at the same time may result in higher cell mortality Gene t...

Страница 16: ...observed When repeated arc discharges within one program occurs it might be impossible for the Nucleofector II Device to complete program execution In this case Err8 Arc discharge significant impacts...

Страница 17: ...e Very weak pulse generated Switch off the device Wait for 2 minutes switch on again repeat the program with a new sample Err7 Supply voltage too low Supply voltage insufficient or device has been swi...

Страница 18: ...e range 15 C to 40 C non condensing Altitude 2000 m above sea level Safety class EN 61010 1 UL 61010A 1 IP 20 Weight 2 8 kg 6 2 lb Dimensions w x d x h 30 x 23 x 11 cm 11 8 x 9 6 x 4 3 in Manufacturin...

Страница 19: ...17 Appendix 5 0 Notes...

Страница 20: ...and for obtaining such license The Amaxa Nucleofector Technology comprising Nucleofection Process Nucleofector Device Nucleofector Solutions Nucleofector 96 well Shuttle System and 96 well Nucleocuvet...

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