LI-COR ODYSSEY Sa, Руководство оператора

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Odyssey® Sa Imager
3) Lower the microplate between the alignment pins until it rests on the microplate carrier and
press the eject button again to retract the microplate carrier.
Starting Microplate Scans
Scans are started by clicking Start on the Acquire ribbon in Image Studio software. Below are the
typical scan parameters that should be used for initial runs with microplates.
The Focus Offset can be set at 3.0 mm when using the plates specified by LI-COR Biosciences.
The Intensity for both 700 and 800 nm channels should be set to 7 for initial scanning. If the
image signal is saturated or too high, rescan using a lower intensity setting (i.e., 4). Likewise, If the
image signal is too low, re-scan using a higher intensity setting (i.e., 8).
For satisfactory images with minimal scan time, use 200 µm for the Resolution . Higher resolution
may be used, but scan time will increase.
More specific scanning guidelines may also be given in the various protocols in the Odyssey Sa
Imager Application Protocols manual.
Scanning Membranes in the Membrane Carrier
The Membrane Carrier allows for scanning blots or gels up to 2-13/16” x 4-1/2” (7.2 x 11.4 cm) in
size. The Membrane Carrier has the same outer dimensions as a standard microplate, but with a
ridge around the edges to prevent liquid from spilling into the Odyssey Sa Imager. The physical
thickness of the Membrane Carrier surface is 3/16” (4.8 mm); however, due to the angle of
incidence of the excitation lasers, the focus offset values suggested below are corrected values to
account for the refractive index of the glass.
Loading a Membrane
1) Before each use, clean the Membrane Carrier with a mild laboratory detergent and rinse thor-
oughly with ultrapure water. Do not use abrasive brushes or cleaning materials on the Mem-
brane Carrier, as they may scratch the Carrier surface. Allow the Membrane Carrier to air dry, or
blot dry with lint-free paper towels.
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