Leica TCS SP8 SMD, Руководство пользователя, Страница: 221 / 270

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Changing Detector Connections on the Scan Head and Router When Using HyD-RLD
24 Changing Detector Cable Connections on the Scan
Head and Router When Using HyD-RLD
If you have a Leica TCS SP8 SMD with MP configuration and external MPD APDs, internal
SP FLIM PMT and HyD-RLDs, you can use the different detectors for different SMD
experiments.
Limitations when using HyD-RLDs
When using external MPD APDs and HyD-RLDs, there are two signals that are transferred
over different cables: the image signal and the SMD signal. The SMD signal cable has to be
connected with the PHR 800 router and the image signal cable with the connection of the
APD printed circuit board on the scan head. When using multiple detectors, it is necessary
that the detectors used are connected correctly with the PHR 800 router and the scan
head's APD printed circuit board. For this purpose, changing the cable is necessary.
The following detectors can be used and have to be connected correspondingly:
• SP FLIM PMT: The SMD signal cables have to be connected with CH 1 IN and CH 2 IN at
the router's CFD input.
• MPD APD (SPAD 1 and SPAD 2): The image signal cable has to be connected at the APD
printed circuit board of the scan head and the SMD signal cable has to be connected to
CH 1 (detector channel 1) and CH 2 (detector channel 2) of the router.
•
HyD-RLD:
The image signal cable has to be connected at the APD printed circuit board
of the scan head and the SMD signal cable has to be connected to CH 1 IN and CH 2 IN
at the router's CFD input.
The following lasers can be used and have to be synchronized with the Pico Harp 300
counting unit:
• MP (infrared laser) : The MP synchronization cable has to be connected to CH 0 of the
counting unit.
• White light laser The white light laser synchronization cable has to be connected to the
counting unit's CH 0.
• Argon laser (if present): The argon laser has no synchronization cable, therefore the MP
or white light laser synchronization cable can remain connected with CH 0 of the
counting unit.
• APDs for FLCS and HyD-RLDs cannot be used simultaneously for
confocal image acquisition
• SP FLIM PMTs and HyD-RLDs cannot be used simultaneously for FLIM
experiments.
NOTICE Damage to the instrument when changing the cable connections is
Switch all electronic components off before changing the cable
connection, as otherwise the detectors or the scan head could become
damaged. Also switch off the LAS AF, the scan head and the detectors.