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F(L)CS Data Acquisition
• The user does not have access to the
Total number of measurements
field. It serves to
visualize the number of FCS measurements carried out ( Figure 181, item 11 ). This number
is the product of
Number of defined points
times
Repetition at point
times
Number of
cycles .
The maximum number of FCS measurements in a measurement series is 1000.
Start the measurement series in LAS AF by clicking the
Run FCS
image acquisition button.
18.4.4 Definition of the File Names Transferred to SymPhoTime during the
FCS Measurement
You can specify the name of the FCS data file used in SymPhoTime in the
Setup
tab in the
Definition of point measurement
field (
Figure 181, item 12
).
The following functions are available:
• Whenever a series of FCS measurements is started, SymPhoTime generates a folder that
houses all FCS measurement files of this series. The name of the folder is composed as
follows:
•
BaseName_Run
Base name : Enables you to specify the base folder and file name used in SymPhoTime
(
Figure 181, item 12
). If the user does not enter a name,
BaseName
will be
automatically used.
Run
: This is a suffix that is added to the file names transferred in SymPhoTime.
In SymPhoTime, no two folders may have the same name. Therefore, the number
given in the
Run
suffix is automatically increased by one whenever a measurement
series is started. The Run suffix can be changed by the user ( Figure 181, item 13 ).
• The file name uses the same BaseName and Run components as the folder. Additionally,
LAS AF will automatically add some more suffixes to the file name that contain the
following information:
•
Cycle
• Measuring point
•
Repetitions at this point
The file name structure is as follows:
• BaseName_Run_Cycle_Measuring point_Repetition
• The Measuring point number is identical to the number used in the image display.
These additional suffixes cannot be modified by the user.
• If Image after each __ cycles is enabled, an image or stack is automatically saved in
LAS AF with the following name:
BaseName_Run_Cycle
Do not use the same name
Do not enter the same
BaseName_Run
name twice. This will create an
error message.