Leica TCS SP8 SMD, Руководство пользователя, Страница: 141 / 270

141
FLIM Data Acquisition
Figure 124: Beam Path Settings in LAS AF: Activating the continuous wave VIS lasers
If the system has an FCS filter wheel, its setting is irrelevant for FLIM, as it is directly in front
of the argon laser and thus does not affect the pulsed excitation.
16.2.2.2 Using Pulsed Diode Lasers (UV, VIS)
• For external FLIM with APDs or an external photomultiplier: Follow the instructions in
Chapter 15.1
. The fluorifier disc can be set to
Empty
as needed. If additional suppression
of the excitation light is desired, follow the recommended settings in Table 19 .
• For internal SP FLIM with SP FLIM detectors: Check the setting of the fluorifier disc. It
contains an additional barrier filter that prevents excitation light from hitting the SP
detection module. Recommended settings are given in
Table 19
in
Chapter 16.2.3
.
16.2.2.3 Using MP Lasers
• For external FLIM at the X1 port and for internal FLIM: Follow the instructions in
Chapter 15.1
. With the fluorifier disc, an MP filter block has to be selected (SP680 or
SP700); the pinhole should be open as far as possible.
• For FLIM detection at NDD position (HyD RLD): The setting of the fluorifier disc, X1 port,
and pinhole play no role.
Prevent light incidence during MP FLIM measurement
During an MP FLIM measurement (particularly with HyD RLD), the light in
the room should be switched off and the shutter of the fluorescence lamp
should be closed. Furthermore, the iris diaphragm on the condenser
should be completely closed.