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Leica CM1520 – Cryostat

Daily Use of the Instrument .................................................................................................................. 31

7.1

Specimen freezing ..................................................................................................................................... 31

7.1.1 Quick freeze shelf ....................................................................................................................................... 31

7.1.2 Stationary heat extractor

(optional) ............................................................................................ 32

7.2

Specimen discs .......................................................................................................................................... 32

7.2.1 Inserting the specimen discs into the specimen head ........................................................................ 32

7.2.2 Specimen orientation ................................................................................................................................ 32

7.3 Sectioning .................................................................................................................................................... 33

7.3.1 Blade holder CE .......................................................................................................................................... 33

7.3.2 Blade holder CE-TC .................................................................................................................................... 39

7.3.3 Knife holder CN ........................................................................................................................................... 40

7.3.4 Knife holder CN-Z with anti-roll guide .................................................................................................... 44

7.3.5 Cleaning the blade and knife holders .................................................................................................... 44

7.3.6 Installing the shelf (movable) (optional) ................................................................................................. 46

7.4

Temperature selection chart (in minus °C) ............................................................................................ 47

7.5 Defrosting .................................................................................................................................................... 48

7.5.1 Automatic defrosting of the cryochamber ............................................................................................. 48

7.5.2 Manual defrosting of the cryochamber .................................................................................................. 48

7.5.3 Manual defrosting of the quick freeze shelf .......................................................................................... 49

7.6

Terminating work ....................................................................................................................................... 49

7.6.1 Terminating daily work .............................................................................................................................. 49

7.6.2 Shutdown for a longer period .................................................................................................................. 50

8. Troubleshooting ..................................................................................................................................... 51

8.1

Error messages in the display .................................................................................................................. 51

8.2

Temperature control button ..................................................................................................................... 52

8.3

Possible sources of error, causes and remedies ................................................................................. 53

8.3.1 Changing the battery ................................................................................................................................. 57

Cleaning, Disinfection, Maintenance ................................................................................................ 58

9.1 Cleaning ....................................................................................................................................................... 58

9.2

Spray disinfection with Leica Cryofect ................................................................................................... 59

9.3 Maintenance ............................................................................................................................................... 60

9.3.1 General maintenance instructions .......................................................................................................... 60

9.3.2 Replacing the fuses ................................................................................................................................... 62

9.3.3 Replacing the fluorescent lamp ............................................................................................................... 63

10.

Ordering Information, Components and Consumables ................................................................... 64

10.1 Ordering information .................................................................................................................................. 64

10.2 Mobile heat extractor –

application ............................................................................................ 89

10.3 Cold extractor –

application ....................................................................................................... 89

11.

Warranty and Service ........................................................................................................................... 90

12.

Decontamination Certificate

(master)

............................................................................................... 91

Table of Contents

Содержание CM1520

Страница 1: ...Instructions for Use Leica CM1520 V1 2 09 2012 English SAP RevC 14 0491 81101 Always keep this manual with the instrument Read carefully before working with the instrument Leica CM1520 Cryostat...

Страница 2: ......

Страница 3: ...n the back of the instrument Leica Biosystems Nussloch GmbH The information numerical data notes and value judgments contained in this manual represent the current state of scientific knowledge and st...

Страница 4: ...1 Installation site requirements 16 4 2 Unpacking instructions for the CM1520 cryostat 17 4 3 Standard delivery 20 4 4 Assembling the handwheel 21 5 Instrument Setup 22 5 1 Electrical connection 22 5...

Страница 5: ...efrosting of the cryochamber 48 7 5 3 Manual defrosting of the quick freeze shelf 49 7 6 Terminating work 49 7 6 1 Terminating daily work 49 7 6 2 Shutdown for a longer period 50 8 Troubleshooting 51...

Страница 6: ...at a prod uct has been tested and fulfills the applicable safety and or performance standards including the relevant stan dards defined or administered by the American National Standards Institute ANS...

Страница 7: ...right position according to your requirements With a pitch of 60 or more the blue quartz sand flows into the ar row shaped indicator window and sticks there permanently Improper handling of the ship m...

Страница 8: ...eze and section speci men material quickly The instrument is not designed for unsupervised storage of specimen material The instrument may only be operated within the scope of its designated use as de...

Страница 9: ...as the designated personnel who operate service or repair the instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings regulations...

Страница 10: ...e glass anti roll plate of the CE and CE TC blade holders also serves as a knife guard 2 3 1 Locking unlocking the handwheel 12 o clock position 6 o clock position 1 2 Fig 2 Fig 3 Rotate the handwheel...

Страница 11: ...ltage must not drop below the values specified in Chapter 3 Technical Data The compressor draws a starting current between 45 and 50 A Therefore theelectriccircuitattheplaceofinstallationmustbeinspect...

Страница 12: ...ial contamination risk the drain tube on the side of the instrument Fig 52 must be inserted in a suitable catch tank At cryochamber temperatures of 0 C to 30 C Spray a MAXIMUM of 10 sprays into the mi...

Страница 13: ...s can cause severe damage to the instrument and its surroundings Replacing the fluorescent lamp Turn the instrument off and disconnect the power plug before replacing the lamps More details are in Cha...

Страница 14: ...range 0 C to 30 C 3 K 3 K Cooling time down to 30 C max 6 hrs at 22 C ambient temperature Defrost Automatic hot gas defrost 1 automatic defrost cycle 24 hours time controlled duration 12 min Refrigera...

Страница 15: ...pprox 135 kg Lamp 50 Hz version Osram Dulux S 11 W 21 Color LUMILUX light white 60 Hz version Osram Dulux S 13 W 21 Color LUMILUX light white All CM1520 instrument variants need the following secondar...

Страница 16: ...is intended for the local power supply The chemicals to be used are easily inflammable and hazardous to health Therefore the installation location must be well ventilated and must contain no sources o...

Страница 17: ...ng instructions for the CM1520 cryostat 4 Setting up the Instrument Lift the outer carton ring 2 upwards and out Remove the foam transport anchors 3 and then pull the dust cover from the instrument Re...

Страница 18: ...e Fig 8 6 Fig 7 Fig 8 5 5 Carefully roll the instrument backwards over the ramp from the pallet Push the instrument to the installation location on the castors 7 7 8 Transport grip points Do not push...

Страница 19: ...ck in all the way 1 1 2 2 2 not visible Fig 9 Transport to the site First check if the location meets the conditions specified in Installation site requirements Transport the instrument to the desired...

Страница 20: ...no 2 5 14 0222 04137 1 Allen key no 3 0 14 0222 04138 1 Allen key no 4 0 14 0222 04139 1 Allen key with spherical head no 4 0 14 0222 32131 1 Allen key no 5 0 14 0222 04140 1 Allen key with handle no...

Страница 21: ...g 10 Tighten the screw 4 using an Allen key 6 mm Attach the cover disk self adhesive not pictured To dismount proceed in reverse order 4 Setting up the Instrument 1 2 4 Fig 10 3 4 The handwheel and al...

Страница 22: ...must not drop below the values specified in Chapter 3 Technical Data The compressor draws a starting current between 45 and 50 A Therefore the electric circuit at the installation site must be inspec...

Страница 23: ...fe holder base on the microtome base plate Set and clamp the blade knife and knife holders in place see Chap 7 3 Open the knife box with the knife and place it in the cryochamber for precooling Place...

Страница 24: ...24 Instructions for Use V1 2 09 2012 5 Instrument Setup 5 3 Overview of the Leica CM1520 4 15 14 16 17 18 19 12 11 10 8 9 12 13 Fig 11 12 20...

Страница 25: ...station optional 10 Stationary heat extractor optional 11 Holder for heat extractor optional 12 Specimen disc 13 Cold extractor optional 14 Section waste tray 15 Brush shelf 16 Orientable specimen hea...

Страница 26: ...ndication of the temperature Program the desired values as described under 6 2 1 to 6 2 3 The software version 0 40 shown here only as an example is displayed in the 4 digit display for the time This...

Страница 27: ...lock the control panel to protect the entered parameters from unintended modifications To lock or unlock hold down for approximately 5 seconds 6 2 Programming the desired values 6 2 1 Setting the time...

Страница 28: ...isplays the target temperature You can now set the desired value using these buttons Pressing the or button for more than 1 sec increases or decreases the chamber tem perature continuously The actual...

Страница 29: ...by the button in the cryochamber temperature selection field Fig 19 6 2 6 Setting the section thickness Fig 20 Adjust the section thickness using the and pushbuttons in the top control panel field see...

Страница 30: ...ion To feed the specimen press and hold the button The LED 2 lights up when the forward end position has been reached 6 4 Control panel 2 Electric coarse feed Move the specimen away from the knife Sta...

Страница 31: ...specimens can be attached The temperature of the quick freeze shelf is always lower than the cryo chamber temperature Cut the specimen to size Apply a sufficient amount of cryocompound to a specimen d...

Страница 32: ...ball shell using the lever 5 Retighten the clamping lever 4 7 1 2 Stationary heat extractor optional Fasten the support 1 Fig 23 of the heat extractor 2 Fig 23 to the left side panel of the cryochamb...

Страница 33: ...e front Inserting the blade holder CE 3 Push the upper part of the blade holder onto the base Clamp the blade holder into place using an Allen key No 4 on the left side 23 Fig 26 23 Fig 25 If an addit...

Страница 34: ...e blade is inserted so that it is centered and lies evenly along the ledge see red arrow in Fig 27 4 Clamp the clamping lever 10 by turning it clockwise see Fig 28 5 Fold the anti roll guide system 4...

Страница 35: ...d Fig 30 Two magnets are attached to the rear side of the guide These point away from the operator after the guide has been inserted towards the rear pressure plate Then insert the blade as described...

Страница 36: ...e blade holder here on the base can be shifted sidewaysinordertouseanotherpart of theblade and to benefit from the entire length of the blade To do so follow these steps 1 Turn the clamping lever 14 t...

Страница 37: ...ow may not lead to optimum sectioning results and can possibly damage the specimen We generally recommend using higher clearance angles for harder specimens and relatively lower clearance angles for s...

Страница 38: ...gh enough Remedy Turn the knurled nut coun terclockwise until the section is pushed between the blade and anti roll guide as shown in Fig III Fig II Section tears and block hits the glass insert after...

Страница 39: ...e glass inserts from above into the bore of the swinging arm 6 in such a way that the pin rests in the notch 3 Push the white plastic plate 9 from below onto the shaft 8 4 Screw the knurled nut 10 fro...

Страница 40: ...e lever 2 to the front Inserting knife holder CN 3 Push the upper part of the knife holder 3 onto the base 1 Clamp the knife holder into place using an Allen key No 4 on the left side 23 Fig 40 1 3 23...

Страница 41: ...ely adjust even greatly worn knives up to a height of 25 mm Once the correct height is obtained tighten the wingscrews 7 in alternating order until both are tight To remove the knife follow these inst...

Страница 42: ...sition a groove 17 is provided in the shaft 16 in which the anti roll guide sits 19 19 18 16 17 Knife guard lateral movement for knife holder CN Clearance angle adjustment The clearance angle adjustme...

Страница 43: ...osen the screws 4 on the underside of the segment arc Fig 44 5 6 Fig 45 Lift off the clamping jaw 5 on the right side caution washers andfeeditintotheadjacent hole 6 Tightenthescrewsfromtheunderside o...

Страница 44: ...nd knife holders 7 Daily Use of the Instrument The height of resharpened knives must be adjusted using knurled screws 3a approx 1 mm under the edge of the clamping jaw Make sure that the knife is para...

Страница 45: ...the segment arc 36 4 11 Fig 47 12 Knife holder CN Fig 48 Occasionally oil the moving parts such as the axes 37 and 38 and the slot 39 with a drop of cryostat oil Type 407 39 38 37 Disinfection Spray...

Страница 46: ...edge Adjust the anti roll plate if necessary see Clearance angle adjustment Attach the rod for the shelf to the inner front side of the cryostat housing using the provided screws 1 and the No 3 Allen...

Страница 47: ...ues as any tissue may require particular adjustments Tissue type 10 C 15 C 15 C 25 C 25 C 30 C Adrenal Bone marrow Brain Bladder Breast fatty Breast little fat Cartilage Cervical Fatty Heart and vascu...

Страница 48: ...frosting of the cryochamber An automatic defrost cycle takes place once in 24 hours You can program the time of the automatic defrost cycle on control panel field 1 see item 6 2 2 7 5 2 Manual defrost...

Страница 49: ...all the specimens from the cryostat Attach the cover for the freeze shelf Remove the plug and close the sliding window Turn out the cryochamber illumination Lock control panel field 1 Fig 14 using th...

Страница 50: ...t breaker and unplug the power plug Open the sliding window to allow the cryochamber to dry Remove all the specimens from the cryostat Lock the handwheel Take the knife blade out of the knife blade ho...

Страница 51: ...display reappears for a longer period After 17 610 hours HELP is output in the real time display to indicate the need for service mainte nance The display alternates between HELP and the real time di...

Страница 52: ...the minimum distance The ventilation slits of the liquefier are dirty Clean air inlet openings see Fig 54 Fig 51 1 After eliminating the possible sources of error push the temperature control button...

Страница 53: ...ubricate sections Specimen not cold enough Knife and or anti roll plate not yet cold enough the section melts as a result Select lower temperature Wait until knife and or anti roll plate have reached...

Страница 54: ...rectly Uneven sections Knife blade damaged Edge of anti roll plate damaged Use different part of the cut ting edge or replace Replace plate Chatter during sectioning Specimen insufficiently frozen ont...

Страница 55: ...ature incorrect for the tissue cut Knife profile inappropriate for the specimen cut Ice buildup in the knife back Handwheel speed not uniform or turned at incorrect speed Knife not clamped tightly eno...

Страница 56: ...r the tis sue being sectioned Blunt part dirt dust frost or rust on the knife Front edge of the anti roll plate damaged Hard particles in the tissue Dirt on back of knife Increase temperature and wait...

Страница 57: ...er the setting values after changing the battery 8 3 1 Changing the battery Problem Cause Remedy No or insufficient refrigeration Compressor defective Leak in the cooling system Inappropriate site con...

Страница 58: ...he storage shelves and the brush shelf for cleaning Remove the closed sliding window from the front by gently lifting it see 9 3 3 Replacing the fluorescent lamp The use of flammable sprays within the...

Страница 59: ...ade from the knife blade holder 3 Remove all specimens microscope slides and tools from the cryochamber 4 Remove debris from the cryochamber Allow the cryochamber to reach the previously selected temp...

Страница 60: ...Clean the instrument every day Weekly Before the following parts can be oiled all section waste and deposits must be carefully removed Oil the plastic coupling 6 Detail 55a with a drop of cryostat oi...

Страница 61: ...ay down and apply a few drops of cryostat oil Distribute the oil by turning the handwheel or using a clean tissue In case of visible pollution such as dust clean the air inlet opening 5 Fig 54 of the...

Страница 62: ...ethescrew driver to screw the sleeve back in as far as it will go Fuse Protection Type F1 Display T 0 25 A F2 Coarse feed T 1 6 A F3 Processor board supply T 1 0 A F4 Peltier element T 6 25 A Fig 56 T...

Страница 63: ...n and push it to the right until it engages in the holder Pushthe lampupwards slightly intotheclip 5 Replace the sliding window Connect the instrument to the power supply again and switch it on 3 4 5...

Страница 64: ...e disposable blades Type 818 1 pack of 50 14 0358 38926 Leica high profile disposable blades Type 818 10 packs of 50 14 0358 38383 Leica low profile disposable blades Type 819 1 pack of 50 14 0358 389...

Страница 65: ...sy Dip staining container yellow 14 0712 40153 Easy Dip staining container blue 14 0712 40154 Easy Dip staining rack gray 14 0712 40161 Blue O ring for color marking 20 mm and 30 mm 10 pcs 14 0477 432...

Страница 66: ...wide 14 0477 42497 Glass insert 50 mm 14 0419 33816 Embedding medium for cryosectioning Jung tissue freezing medium 125 ml 14 0201 08926 Cryostat oil Type 407 250 ml 14 0336 06100 Leica Cryofect low t...

Страница 67: ...t rails for long and short knives Anti roll guide and adjustable knife guard Order No 14 0477 42358 Fig 61 10 Ordering Information Components and Consumables Fig 62 Knife holder CN Z For conventional...

Страница 68: ...ig 65 Fig 64 Blade holder CE for low profile disposable blades without clear ance angle adjustment including blade knife holder base and pressure plates Order No 14 0419 33992 10 Ordering Information...

Страница 69: ...4 0419 19426 Knife support for knife holder CN for long knives Order No 14 0419 19427 Fig 67 Blade holder pressure plate 22 for high profile microtome blades Order No 14 0491 48004 Fig 68 10 Ordering...

Страница 70: ...ts and Consumables Disposable blades Leica TC 65 Leica TC 65 microtome disposable blade system for sectioning hard specimen materials The Leica TC 65 carbide metal disposable blades were specially dev...

Страница 71: ...216 07092 Knife 16 cm length profile d Note including knife case 14 0213 11140 Order No 14 0216 07132 Knife 16 cm carbide metal blade profile d Note including knife case 14 0213 11140 Order No 14 0216...

Страница 72: ...ngth of 18 5 22 or 30 cm for carbide metal or SM2500 knives only for 1 knife only for 1 knife Order No 14 0213 11141 Case with cryoembedding system includes 3 embedding well bars with recesses in thre...

Страница 73: ...dding well bar set with small recesses consisting of 1 embedding well bar recesses 18 mm 4 specimen stages small 2 heat extractors 8 dispensing slides Order No 14 0201 39116 Embedding well bar set wit...

Страница 74: ...01 39120 Height 25 4 mm with 4 recesses size 24 mm stainless steel Order No 14 0201 39121 Height 25 4 mm with 4 recesses size 30 mm stainless steel Order No 14 0201 39122 Height 25 4 mm with 2 recesse...

Страница 75: ...6 Dispensing slide for Dr Peter s cryoembedding system pack of 8 Order No 14 0201 39127 Fig 85 10 Ordering Information Components and Consumables Specimen stage rectangular stainless steel small 28 mm...

Страница 76: ...er No 14 0477 43248 Blue O ring for color marking the 40 mm specimen disc pack of 10 Order No 14 0477 43249 Red O ring for color marking the 40 mm specimen disc pack of 10 Order No 14 0477 43250 Blue...

Страница 77: ...04138 1 Allen key No 4 0 14 0222 04139 1 Allen key No 5 0 14 0222 04140 1 Allen key No 6 0 14 0222 04141 1 Allen key No 1 5 14 0222 10050 1 Allen key ball cage No 4 14 0222 32131 1 single head wrench...

Страница 78: ...ite pack of 6 Order No 14 0712 40150 Color pink pack of 6 Order No 14 0712 40151 Color green pack of 6 Order No 14 0712 40152 Color Yellow pack of 6 Order No 14 0712 40153 Color Blue pack of 6 Order N...

Страница 79: ...Cryostat Specimen disc 20 mm Order No 14 0370 08636 25 mm 14 0416 19275 30 mm 14 0370 08587 40 mm 14 0370 08637 55 mm 14 0419 26491 50 x 80 mm 14 0419 26750 Fig 90 10 Ordering Information Components...

Страница 80: ...lf movable optional 4 Blade holder CE with anti roll guide 4a anti roll guide also acts as knife guard optional 5 Brush shelf 4 6 2 5 Fig 91 3 7 2 4a 6 Object head directional 7 Waste tray Enlarged de...

Страница 81: ...ig 95 Fig 94 Heat extractor mobile Order No 14 0443 26836 Transfer block for specimen discs small 5 holes combinable with transfer block for specimen discs large Order No 14 0491 47787 Fig 92 10 Order...

Страница 82: ...ngeableglassinsertsCE 100 m spacer Recommended section thickness 5 m 50 m For blade holder CE 14 0491 47873 14 0419 33992 Order No 14 0419 35693 Fig 97 Anti roll guide system CE glass 70 mm consisting...

Страница 83: ...of Glass insert 70mm MetalframeforexchangeableglassinsertsCE 50 m spacer Recommended section thickness up to 4 m For blade holder CE 14 0491 47873 14 0419 33992 Order No 14 0419 37258 Anti roll guide...

Страница 84: ...blade holder CE BB 14 0477 43005 Order No 14 0477 42497 Glass insert 50 mm For knife holder CN 14 0477 42358 Order No 14 0419 33816 Embedding medium for cryosectioning Jung tissue freezing medium 125...

Страница 85: ...14 0419 33816 Cut resistant safety gloves 1 pair size S Order No 14 0340 40859 Cut resistant safety gloves 1 pair size M Order No 14 0340 29011 Fig 107 Heat extractor stationary assembly Order No 14 0...

Страница 86: ...knife Profile c 12 cm length Order No 14 0337 08688 Lapping device for knife Profile c 10 cm length Order No 14 0337 08689 Lapping device for knife Profile d length 16 cm Order No 14 0337 08693 Fig 10...

Страница 87: ...ing stone coarse blue green with scraper approx 25 x 5 5 cm Order No 14 0210 04071 Metal case for storing lapping stones Order No 14 0337 04070 or 14 0337 04071 Order No 14 0210 04073 Strop Type Heide...

Страница 88: ...delberg Order No 14 0285 07057 Fig 115 Fig 116 10 Ordering Information Components and Consumables Strop paste red Order No 14 0209 04064 Fig 117 Storage system movable for installation in the front pa...

Страница 89: ...c 5 into the corresponding bore 7 on the cold extractor After about 20 seconds the frozen specimen can be removed from the specimen disc with forceps If the cap is too loose readjust it with the small...

Страница 90: ...The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Service information If you are in need of techni...

Страница 91: ...ms or serviced on site must be cleaned and de contaminated in the appropriate manner Since it is not possible to decontaminate for prion diseases such as CJD BSE CWD etc equipment exposed to specimens...

Страница 92: ...Leica branch office Leica Internal Use If applicable note corresponding Job and RAN RGA numbers Job Sheet No ____________________ SU Return Goods Authorization ____________________ BU Return Authoriza...

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