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Aperio GT 450 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2022
78
Chapter 6: Troubleshooting
2.
Check the stain quality.
3.
Check that the slide and coverslip thickness meet the requirements. See the
Aperio GT 450 DX Scanner
Specifications
.
4.
Check for common slide preparation issues and verify:
`
Coverslip is not missing or overhanging.
`
Labels are not overhanging or on wrong side.
`
There is only one label applied to the slide.
`
Slide is clean.
5.
If every slide has the same error, or if the issue persists after following the previous steps, call Leica Biosystems
Technical Services.
Image Quality
`
Cause:
The scanner’s Auto-Image QC feature detected an image quality issue.
Follow these steps in order until the issue is resolved:
1.
Review the scanned slide image in your viewing software.
2.
Check for common slide preparation issues:
`
Coverslip is not missing or overhanging.
`
Labels are not overhanging or on wrong side.
`
There is only one label applied to the slide.
`
Slide is clean.
3.
Scan the slide again. When the scan is finished, do not remove the rack from the scanner.
4.
Review the newly scanned slide image in your viewing software.
5.
If the issue persists, display the slide in Slide View, and tap
Scan Entire Slide
. (To scan the whole area for a rack of
“Scan Entire Slide for Whole Rack” on page 37
.)
6.
Check if other slides are in focus.
7.
If all slides are out of focus, clean the objective. (See
“Clean the Objective and Koehler” on page 44
)
8.
If the issue persists after following the previous steps, call Leica Biosystems Technical Services.