Appendix B
B-8
D960R3
User’s Manual Probot
B.6
Additional Hints/Troubleshooting
For the proper handling of a fractionation task and to make sure that no peak is
lost, it is important to calculate or measure the delay time between the flow cell
of the detector (where the peak is detected) and the fractionation needle (where
the peak then will be collected).
If two Peak Start (TTL 1) signals are generated immediately after each other, the
Probot moves to the next vial.
If two Peak End (TTL 2) signals are generated immediately after each other, the
Probot moves the table to the next vial or stays in the waste vial if a waste
position is selected.
Some older
µ
Carrier version (e.g. versions lower than 1.70) do not allow
collection in only one waste vessel; it requires that you define as many waste
positions as collect positions (Section B.3 ). To get only one ‘virtual’ waste
position with the proper amount of vessels, you can program a very small
distance (e.g. only 0.1 mm) between each vessel.
For additional information refer to the
µ
Carrier user’s manual (e.g. Fractionation
Task, Section 5-51), the CHROMELEON on-line help (e.g. tutorial, see trigger)
and to the UltiMate User’s Manual.
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