LC Packings Probot 161403, Руководство пользователя

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Installation and Getting Started
User’s Manual Probot
D960R3
2-25
2.8 µCarrier – Basics Steps
The following sections discus the basis steps of the µCarrier software. We show
how to
• Create a New Sample Carrier using the Sample Carrier Wizard – Section 2.8.1.
• Create a New Tray using the Tray Setup Wizard – Section 2.8.2.
• Modify an Existing Tray Setup using the Tray Editor – Section 2.8.3.
•
Create a New Application using the Wizard – Sect. 2.8.4.
• Control the Table Position – Section 2.8.5.
•
Measure the Column (X) and Row (Y) Distance – Section2.8.6.
The various steps are explained in detail using the following typical application
example:
• A table with six rectangular MALDI target plates with 24 x 24 spots is used
for the fractionation of a NanoLC run. The LC flow rate is 300 nL/min, the
distance between columns and rows is 1.45 mm and the spot diameter is 1
mm.
•
The fraction collection time is 20 sec.
• A MALDI matrix solution is added at a flow rate of 1.2 µL/min (= 4 times the
LC flow rate) along with the fractionation of the mobile phase.
• The start of the fractionation is synchronized with the injection of the UltiMate
Capillary HPLC system and the start signal is delayed by 5 minutes.
• Between the runs the needle should remain in a wash vial to prevent
crystallization of the matrix solution. Dispensing 10
µ L of matrix solution prior
to fractionation will prevent any matrix dilution.
• All available spots will be used.
The application discussed here corresponds to the application that was used to
shown in the ‘µCarrier – First Steps’ section. Please refer also to the user’s
manual of the
µ Carrier software for more detailed information.