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LAMBDA OMNICOLL Fraction Collector - Sampler

  

Operation MANUAL 2023.01

 

 

 

www.lambda-instruments.com

 

 

31

 

 

11 APPENDIX 

11.1 RS communication protocol for LAMBDA OMNICOLL fraction 

collector-sampler 

11.1.1 Format of data sent by the PC to the fraction collector and back 

Data sent by the PC:   

 

 

 

#ss mm a xxxx qs c 

Data sent back by the fraction collector: 

 

<mm ss a xxxx qs c 

where, 

# 

is the first sign of a command sent by PC 

< 

is the first sign of a message sent by fraction collector 

ss 

is the address of the fraction collector (slave) 

mm 

is the address of the PC (master) 

A 

is the command (see 

section 11.1.2

) 

xxxx 

is the data value (4 ASCII numbers from 0 to 9; sent from the highest order digit to 
the lowest order digit) 

Qs 

is the control sum in HEX format (2 ASCII characters of the type 0…9ABCDEF) 

C 

is the end sign cr (carriage return) The collector will fulfil the task and block any 
manual command on the front panel. 

 

11.1.2 Commands 

# ss mm 

r 

qs c 

start (run) 

# ss mm 

e 

qs c 

activates remote control of the collector (front panel deactivated) 

# ss mm 

g 

qs c 

activates local mode (front panel activated) 

# ss mm 

s 

qs c 

stop 

# ss mm 

f 

qs c 

step forward 

# ss mm 

b 

qs c 

step back 

# ss mm 

w 

qs c 

step in actual moving direction (depending on LINE or MEAN 
setting) [corresponds to pressing the STEP button] 

# ss mm 

l 

qs c 

step to next line 

# ss mm 

h 

qs c 

“high” mode 

# ss mm 

u 

qs c 

“normal” mode 

# ss mm 

m 

qs c 

“MEAN” collection mode (meander or zigzag collection mode) 

# ss mm 

v 

qs c 

“LINE” collection mode (collects fractions always from left to right) 

# ss mm 

i 

qs c 

“ROW” collection mode, the collector moves only from row to row 

# ss mm 

d 

qs c 

unit setting 

– 0.1 minute step time setting (XXX.X) 

# ss mm 

j 

qs c 

unit setting 

– minute step time setting (XXXX) 

# ss mm 

o 

qs c 

open valve 

# ss mm 

c 

qs c 

close valve 

# ss mm 

a 

qs c 

division coefficient setting “1” 

# ss mm 

k 

qs c 

division coefficient setting “1/60” 

 

 

# ss mm 

p 

xxxx qs c 

number of pulses from pump or drop counter 

# ss mm 

t 

xxx.x qs c 

collection time (in 0.1 minute steps) 

# ss mm 

t 

xxxx qs c 

collection time (in minute steps) 

# ss mm 

q 

xxx.x qs c 

pause time between two fractions (in 0.1 minute steps)  
(fraction collect

or automatically enters “high” mode) 

Содержание OMNICOLL

Страница 1: ...LAMBDA OMNICOLL Fraction Collector and Sampler Operation MANUAL LAMBDA OMNICOLL Fraction Collector Sampler...

Страница 2: ...onstruction with tubes easily accessible from all sides Multi column attachment for simultaneous fraction collection optional Low voltage power supply battery operation possible for field applications...

Страница 3: ...in row 16 3 3 2 Coding Y axis for row distance 17 3 4 Control Panel of OMNICOLL Fraction Collector 17 3 4 1 Selection of linear meander like or row collection 18 3 4 2 Time resolution 0 1 or 1 minute...

Страница 4: ...rst tub sheet into the slots on one of the shorter sides of the yellow support Figure 1 1 2 Push the second tub sheet into the slots on another shorter side of the yellow support Figure 1 1 3 Insert t...

Страница 5: ...ails The distance from the edge should be the same on the left and right sides The gear wheels of the control unit must engage correctly in the gears of both rails Be sure that nothing will disturb th...

Страница 6: ...wn Figure 1 2 3 Slide the O ring on the PTFE tubing Figure 1 2 4 Push the tubing through the tubing guide and make sure a few mm 5mm of PTFE tubing reach out of the tubing guide so that the drops form...

Страница 7: ...the first lower part unit and the front side of the second lower part unit Insert the coupling bars Art No 6912 on their place and fix with the four bolts Be aware that you must remove the fixed stop...

Страница 8: ...ven more effluents of multiple chromatography columns This multi stream assembly is technically very simple and polyvalent It allows an easy adaptation of various experimental needs directly by the us...

Страница 9: ...t a convenient height above the tubes is obtained Fix the tubing guide to the holder using the corresponding socket key hex key Figure 1 4 1 10 Place the tubing guide holder at the desired fraction po...

Страница 10: ...le up to 8 channels Examples of customized multi stream fraction collector LAMBDA OMNICOLL Version 2022 Figure 1 4 2 1 Front side of the customized multi channel fraction collector LAMBDA OMNICOLL for...

Страница 11: ...you need to assemble the moving arm holder Figure 1 4 2 6 The holder is fixed with two screws using a hexagonal key Figure 1 4 2 7 The moving arm holder attaches to the black rectangle attached to th...

Страница 12: ...com 11 Figure 1 4 2 11 The tube guide is mounted on a moving arm in accordance with the selected position For each stream one tube guide art no 6950 is needed Figure 1 4 2 12 The tube guide is fixed...

Страница 13: ...rotated Slightly loosen the nut C so that the PTFE tubing D can move freely inside the tubing guide B Screw the drop counter E on the lower end of the tubing guide B Tighten the tubing guide holder sc...

Страница 14: ...nsing tubing is about 1 cm above the tubes or recipients Place the tube rack on the fixing mat Figure 1 1 8 so that the first tube is just under the tip of the tubing and the edge of the rack is paral...

Страница 15: ...e fraction positioning Figure 3 1 2 The fraction positions are indicated by white lines drawn on the black magnetic coding band as shown The white lines should be about 2 mm broad and full length Thes...

Страница 16: ...etic coding bands programmed with white lines for the desired tube racks are provided with the OMNICOLL Fraction collector Figure 3 2 1 Place the longer and narrower coding band Y axis on the left inn...

Страница 17: ...lines correctly on the pre marked places Reserve approximately 2cm of the coding band for the beginning and end position signals Figure 3 3 3 Mark the beginning and end signals on the lower and upper...

Страница 18: ...ing band on the left internal side of the metal frame just under the gear bar If the fraction collection should be stopped at the last row then make the last line broader approximately 1 cm thick This...

Страница 19: ...he collecting arm does not move within the row The collector moves only from row to row This collection mode is mainly used in the simultaneous multi channel fraction collection mode Figure 3 4 1 Pres...

Страница 20: ...ion is of approx 1 mm 3 4 2 Time resolution 0 1 or 1 minute The time resolution of the OMNICOLL fraction collector can be selected in the following way Figure 3 4 6 Press the button COUNT TIME while c...

Страница 21: ...n of motor are counted In the second setting sixty drops or impulsions give just one signal Figure 3 4 10 Press the button START while plugging the power supply into the mains Figure 3 4 11 div and th...

Страница 22: ...tions of precise volume from 0 05 to 500 ml or 0 6 to 30 litres per fraction The heart of these pumps is a stepping motor or BLDC motor controlled by a generator of electric impulses microprocessor Af...

Страница 23: ...ong connection because the connectors will not fit to the wrong sockets Figure 3 6 1 Connect the peristaltic pump remote control cable to the communication module box Figure 3 6 2 With the COUNT TIME...

Страница 24: ...sed for the collection of samples with a time interval between consecutive fractions varying between 0 1 min and 16 6 hours 999 9 min or 1 min and 166 hours 9999 min This is particularly useful during...

Страница 25: ...ction collection is started by pressing the START button The collector will take the number of samples as programmed under the NUMBER setting and will stop for the time programmed under the PAUSE sett...

Страница 26: ...alarm The fraction collector can take one or several samples after detection of a signal 3 to 12 V or 12 to 30V with a 3300 ohm resistor switched in series The LAMBDA peristaltic pump will be activat...

Страница 27: ...cording to your needs This makes it possible to use even largest containers for taking fractions For such applications a modified tubing holder can also be made On demand we can also supply an adapter...

Страница 28: ...Collector takes a 1 to 999 fractions after a single external voltage pulse of 3 12 V or 12 30 V with a 3300 ohm resistor Capacity Any tube rack or container type with a surface smaller than 45x31cm U...

Страница 29: ...10 000 ml hr programmable 6910 Remote control cable for fraction collector analog 6910 rs RS 232 connection cable 6911 Communication module for pump switching and RS 232 connection 6912 Set for keepin...

Страница 30: ...for Watson Marlow 205U peristaltic pump 4810 LongerBT Remote control cable for Longer BT 100 1 peristaltic pump 4810 WM PF6 Remote control cable for Watson Marlow Flexicon PF6 pump drive peristaltic p...

Страница 31: ...must be returned with a complete description of the defect or problem In order to send back the equipment for repair you will need a returns authorization number from LAMBDA The customer will send th...

Страница 32: ...vates remote control of the collector front panel deactivated ss mm g qs c activates local mode front panel activated ss mm s qs c stop ss mm f qs c step forward ss mm b qs c step back ss mm w qs c st...

Страница 33: ...time xxxx in minute steps resp number of pulses or fractions collector is in stand by mode mm ss R xxx x qs c time xxx x in 0 1 minute steps collector is in operation mm ss R xxxx qs c time xxxx in mi...

Страница 34: ...s of the fraction collector To change the address press the buttons under the display until the desired number is obtained Confirm your choice by pressing the button SET 11 3 RS connection scheme The...

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