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Semi-Micro Osmometer K-7400S instructions, V3700

12. Technical data

12.1 Main features

Feature

Specifications

Sample volume

50–150 µl

Solvent

Water (HPLC quality)

Measurement time

ca. 2 min

Measurement range 0–2000 mOsmol / kg

Precision

SD ≤ 4 mOsmol / kg [0–400 mOsmol / kg]
RSD ≤ 1.0 % [400–2000 mOsmol / kg]

Linearity

± 1 % [0–1500 mOsmol / kg]
± 1.5 % [0–2000 mOsmol / kg]

Calibration

Two-point calibration
(0 mOsmol / kg and one free selectable

osmolality)
optional:
Three-point calibration
(0 mOsmol /kg and 2 free selectable

osmo lalities)

Measuring

procedure

Completely automatic after sample insertion

12.2 Communication

Feature

Specifications

Port

RS-232

Control

Keypad (LED screen, 2 rows with 24 carac ters),

software

Содержание K-7400S

Страница 1: ...Freezing Point Osmometer Instructions K 7400S Semi Micro Osmometer Document no V3700...

Страница 2: ...CET Fax 49 30 8015010 Email support knauer net KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 Internet www knauer net E Mail info knau...

Страница 3: ...acking and setup 11 5 1 Location requirements 11 5 2 Unpacking the device 11 5 3 Power supply 12 6 Startup 14 7 Operation 15 7 1 Connecting the measuring head 15 7 2 Standby position of measuring head...

Страница 4: ...ple list 32 10 6 Setup 33 10 7 Working parameters 33 10 8 Report Graph 35 10 9 Search function 35 11 Maintenance and care 37 11 1 Cleaning the stirring wire and the thermistor 37 11 2 Function checks...

Страница 5: ...Semi Micro Osmometer K 7400S instructions V3700 Table of Contents iv 14 5 Disposal 44 14 6 KNAUER software license agreement 44...

Страница 6: ...ally can be equipped with a printer Furthermore the device can be controlled via the EuroOsmo 7400 software 1 2 Intended use Note Only use the device for applications that fall within the range of the...

Страница 7: ...Function START STOP Starts a measurement or calibration run If this button has no specific function on the screen it causes to return on the main screen With this button every running measurement can...

Страница 8: ...icro Osmometer K 7400S instructions V3700 Product information 6 1 3 3 Rear view Legend 1 RS 232 connector 2 Measuring head con nector 3 Ventilator 4 Power switch 5 Power connector Fig 2 Rear view 5 4...

Страница 9: ...KNAUER or a com pany authorized by KNAUER Freezing Point Osmometer K 7400S Power cable RS 232 connection cable Measuring head Pack of sample tubes 400 mOsmol kg calibration solution 850 mOsmol kg cali...

Страница 10: ...e companies for laboratory work Power cable Defective power cables are not to be used to connect the device and the power supply system Power strip If several devices are connected to one power strip...

Страница 11: ...repair you are required to decontaminate the device in a technically correct manner All contaminated devices must be properly decontaminated by a special ist company or the operating company before t...

Страница 12: ...mbol Meaning Warnng Signs Electric shock hazard Electrostatic discharge hazard damages to sys tem device or components can occur Biohazard CE Mark A device or system marked with CE fulfills the produc...

Страница 13: ...vice on one side At least 10cm if there are devices set up on both sides At least 15cm to the cooler fan on the rear The device should not be exposed to major temperature fluctuations during measureme...

Страница 14: ...hat the specifications stated in Technical Data are met But check beforehand to use power cables admitted for use in your count ry Replace defective power cables only with accessories from KNAUER Deta...

Страница 15: ...onnect the power plug The osmometer needs a three core power plug Electric shock Danger of electric shock from damaged cables Make sure that the power cable is not subjected to high forces The power c...

Страница 16: ...in injuries Avoid direct contact with the cooling compartment Wear protective gloves Electronic defect Damage to the electronics is possible if the device is switched on when it is connected or discon...

Страница 17: ...a stirring wire and the thermistor as temperature sensor The measuring head cable is to be plugged into the connector on the back of the device The sample tube filled with the exact volume of calibrat...

Страница 18: ...Note Generally the measuring head should be stored with the attached sample tube While working with the osmometer use the four columns on top of the device for temporarily set down the measuring head...

Страница 19: ...ture rea ches the freezing point 0 C in case of pure water Fig 6 Fig 6 Temperature time curve for pure water The freezing point of a solution is below 0 C Fig 7 The freezing point depression T is a me...

Страница 20: ...us field As shown in Fig 8 it will take you to the relevant menu points 7 5 Menu guide Starting with the cursor on the rhombus field further menus become accessible in an endless loop by pushing the v...

Страница 21: ...e calibration points The first field is fixed at 0000 At least 2 calibration points are necessary for regular mea surements From this screen the calibrat ions will be started see Performing the cali b...

Страница 22: ...5 08 24 35 After a calibration run you get the following print Calibrated 1234 mOsmol 23 03 15 07 36 17 If a measurement is canceled due to an error the screen displays the cor responding error messag...

Страница 23: ...mple temperature and the elapsed time The run can be interrupted at any time by pressing the STOP button thermistor 9 99 C 00 56 3 After finishing the calibration the screen displays the message CALIB...

Страница 24: ...tion point remains active until a new valid value for the selec ted field is created on the CALIBRATE screen If a calibration run is not suc cessfully finished the last calibration value becomes react...

Страница 25: ...g the sample volume 7 8 Measuring sample solutions 7 8 1 Performing a sample measurement Prepare a measurement with a sample solution see 7 6 Preparing a mea surement on page 20 1 To perform measureme...

Страница 26: ...uid The sample solution should stand upright in the sample tube 4 Handle the thermistor always very carefully Do not use abrasive materi als for cleaning The thermistor should be cleaned occasionally...

Страница 27: ...ration of dissolved particles which leads to increase of osmolality of a solution Calibration solu tions should be used not longer than 30 60 min after the tubes were opened We recommend using KNAUER...

Страница 28: ...evice The Operation Qualification includes an extensive functionality test accor ding to KNAUER standard OQ documents The Operation Qualifica tion is a standardized document and free of charge It is n...

Страница 29: ...measured value is outside the acceptable range Usually a malfunction of the thermistor Contact the Technical Support NOT CALIBRATED No calibration points are available See section Measurement cali br...

Страница 30: ...y ultrasound Note Calibration and measurement have to be performed with the same temperature settings and equal volume 9 3 Non freezing of the sample If the sample does not freeze one of the following...

Страница 31: ...nstallation of EuroOsmo 7400 The program runs under Windows 7 8 and 10 but also with older operat ing systems back to Windows XP 1 Insert the EuroOsmo 7400 CD into the CD ROM drive 2 Wait for 30 secon...

Страница 32: ...ware 30 10 2 Main screen After starting the program you will see the main screen of the software Fig 9 Main screen of EuroOsmo 7400 Software If the osmometer is not connected or switched off you will...

Страница 33: ...chosen column After each bar code scan the cursor switches to the next sample line 10 4 Run Settings Command Explanation Select the number of the sample to be mea sured from the sample list Start but...

Страница 34: ...bar at the bottom a Comments column becomes accessible for detailed informa tion The samples will be measured in the order of the list and the results are filled in and saved Fig 12 New sample list Do...

Страница 35: ...e COM port to which your osmometer is connected 10 7 Working parameters Command Explanation Monitoring view The entered value defines the scale of the moni tor by its final value in minutes The temper...

Страница 36: ...units valid for the measurement sequence The choice is mOsmol C F and If F is selected the values of the freezing level and cooling limit are automatically changed to F By clicking on the Apply butto...

Страница 37: ...n an explorer win dow appears to edit the subtitles Additionally you can format the subtitles here Graph Settings Area for settings of the graph design A click on one of the four buttons will open the...

Страница 38: ...in subfolders Search subjects are the operator the sample name and or the sample code As wildcard characters can be used for single characters and for undefined areas before and or after to the defin...

Страница 39: ...n the responsibility of the user 11 2 Function checks of cooling capacity The cooling capacity can be checked by measuring the temperature in the cooling cavity 13 to 16 C during cooling down and 20 C...

Страница 40: ...tialized by a start of the stirring wire within 150 seconds If the temperature of 13 C cannot be obtained the device must be returned for repair Note The shape of the stirring wire is essential for a...

Страница 41: ...l data 11 4 1 Disconnecting the power supply The device has been switched off Process 1 Pull the power plug out of the socket and afterwards out of the device 2 Pack the power cable together with the...

Страница 42: ...mol kg 0 400 mOsmol kg RSD 1 0 400 2000 mOsmol kg Linearity 1 0 1500 mOsmol kg 1 5 0 2000 mOsmol kg Calibration Two point calibration 0 mOsmol kg and one free selectable osmolality optional Three poin...

Страница 43: ...structions V3700 12 3 General Feature Specifications Power supply 100 240 V 50 60 Hz 70 W Dimensions 160 x 182 x 340 mm Weight 5 3 kg device measuring head Ambient conditions Temperature range 10 35 C...

Страница 44: ...zing point osmometer K 7400S A0006AC Power cable 230V M1479 RS 232 Connection cable M1702 Plain printer A3711 K 7400S Instructions V3700 Measuring head for plastic sample tubes A0840 2 Pack of 100 pla...

Страница 45: ...y for the device is stipulated by contract During the warranty period any components with material or design related defects will be replaced or repaired by the manufacturer free of charge Please conn...

Страница 46: ...roperly According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactured by KNAUER are marked as waste electri cal and electronic equipment 160214 KNAUER as a company is r...

Страница 47: ...OR ANY DERIVATIVE WORKS BASED UPON THE SOFTWARE OR ANY PART THEREOF The Licensee must not remove from the program firm names trademarks logos copy right notices and other markings or devices which ser...

Страница 48: ...special incidental or consequential damages so the above limitation or exclusion may not apply to the Licensee Any liability is in any case limited to the license fee 8 Limitation of Remedies KNAUER s...

Страница 49: ...00 For all controversies out of this Agreement the District Court Berlin shall have exclusive jurisdiction The applicable law shall be that of the Federal Republic of Germany German Civil Code excludi...

Страница 50: ...UER instructions online www knauer net library KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KN...

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