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Maintenance and care

BlueShadow Detector 40D Instructions V7647

8.4 Cleaning the flow cell

Increased baseline noise and reduced sensitivity can be a result of a

dirty flow cell . Often it is sufficient to rinse the flow cell to restore optimal

sensitivity .

Note:

Dirty lenses or fiber optic connectors could falsify the measure-

ment . Do not touch the lens or the fiber optic connector lends with bare

hands . Wear gloves .

8.4.1 Rinsing the flow cell

Flow cell is removed from the detector .

Waste tube is connected to the flow cell .

The following solvents are recommended for rinsing:

Diluted HCl (1 mol/l)

1 ml/l NaOH aq .

Ethanol

Acetone

Syringe

Procedure

Process

Fill the syringe with eluent .

Inject it into the inlet of the flow cell and allow it to act for

5 minutes .

Fill the syringe with water and rinse the inlet .

Use a nitrogen stream to dry the flow cell .

The flow cell has been rinsed .

Check if the baseline noise has decreased .

If the rinsing does not have the desired effect, disassemble the flow

cell to clean the lens .

Prerequisite

Solvents

Tools

Result

Next steps

Содержание BlueShadow Detector 40D

Страница 1: ...Detector 40D Instructions Document no V7647 HPLC HPLC...

Страница 2: ...809727 111 workdays 9 17h CET Fax 49 30 8015010 E mail support knauer net KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 Internet www...

Страница 3: ...oreseeable misuse 3 2 2 User qualification 3 2 3 Operator responsibility 4 2 4 Personal safety equipment 4 2 5 Working with solvents 5 2 5 1 General requirements 5 2 5 2 Contamination by health threat...

Страница 4: ...th LAN 18 4 6 4 Configuring the router 19 4 6 5 Integrating LAN into the company network 20 4 6 6 Controlling several systems separately in LAN 20 4 7 Remote control 21 4 7 1 Connector assignment 21 4...

Страница 5: ...6 Functionality tests 45 6 1 Installation Qualification IQ 45 6 2 Operation Qualification OQ 45 7 Troubleshooting 46 7 1 LAN 46 7 2 Possible problems and solutions 47 7 3 System messages 48 8 Mainten...

Страница 6: ...outdated PETP 61 Polyimide Vespel 62 Ethylene tetrafluorethylene copolymer ETFC Tefzel 62 Perfluorethylenpropylen Copolymer FEP Perfluoralkoxy Polymer PFA 62 Polyoxymethylene POM POM H TF 62 Polypheny...

Страница 7: ...these and other instructions from the KNAUER web site www knauer net library 1 2 Signal words Possible dangers related to the device are distinguished in personal and material damages Symbol Meaning D...

Страница 8: ...e In case you notice any damage inform the Technical Support and the shipping company within three workdays 1 4 3 Warranty conditions For information on warranty please refer to our general terms and...

Страница 9: ...t guarantee the functionality and safety of the device Operation in potentially explosive areas without special and additio nal explosion protection Contact the KNAUER Customer Support for more inform...

Страница 10: ...learly regulate and specify responsibilities for installation operation troubleshooting maintenance and cleaning and set clear rules Ensure that all personnel who work with the device have read and un...

Страница 11: ...s could get onto hot surfaces in the interior Degas solvents before use and observe their purity 2 5 2 Contamination by health threatening solvents Contamination with toxic infectious or radioactive s...

Страница 12: ...wet rooms 2 7 Maintenance care and repair Avoiding electric shock Before performing any maintenance and ser vice work disconnect the device from the power supply Tools Use only tools recommended or pr...

Страница 13: ...quest form and decontamination report Devices which are shipped without the completed document Service request form and decontamination report will not be repaired If you would like to return a device...

Страница 14: ...able independent of the selected wavelength Automatic recording and storage of the device specific characteristics that are important for GLP Good Laboratory Practice and OQ Opera tion Qualification o...

Страница 15: ...chromatography software at the worksta tion or the keypad Legend 1Status LED 2Keypad 3Flow cell with holder Fig 1 Front view 3 3 2 Rear view The rear of the device contains the mains power connection...

Страница 16: ...stem device or components can occur A device marked with CE fulfills the product spe cific requirements of European directives This is confirmed in a Declaration of Conformity Testing seals in Canada...

Страница 17: ...tray as these parts are loosely attached to the device Keep the included packing list for repeat orders Keep the original packaging for safe storage or transportation 4 2 Ambient conditions 4 2 1 Oper...

Страница 18: ...riefly when the device is switched on Main connection The electrical power supply at the operation site must be connected directly to the nearest main power connection Grounding The connectors for the...

Страница 19: ...ocess Figure 1 Unscrew the knurled head screws 3of the cover plate 4 Hold the flow cell securely with your hand during this procedure 2 Pull out the slide with the flow cell 5towards the front 3 Remov...

Страница 20: ...roceed as fol lows Procedure Process Figure 1 Remove the threaded ring 1using a hexagonal spanner 2 Remove stainless steel cover 2and the PEEK spacer 3 3 Insert the stainless steel cover again and car...

Страница 21: ...mm Please use a clean cloth and avoid touching the light guide with your fingers 4 Push the light guide together with the pressure bushing back into the cell 5 Insert the PEEK spacer 3fol lowed by the...

Страница 22: ...ure 1 Push the capillary 2through the fitting 1 2 Push the clamping ring 3 onto capillary Fig 6 Capillary fitting 3 Manually screw together the capillary 5and the flow cell 4 Tighten the fitting with...

Страница 23: ...n assigned by IPv4 IPv6 is not supported This chapter describes how to set up a chromatography system in a local area network LAN and how a network administrator can integrate this LAN into your compa...

Страница 24: ...lick on the button Properties 6 Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically 7 Clic...

Страница 25: ...ays switched on in the same order Set the router properties see section 4 6 4 4 6 4 Configuring the router The router is preset at the factory Information about address user name and password is noted...

Страница 26: ...parately in LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one chromatography systems are connec ted to the same LAN and you plan on controlling...

Страница 27: ...2LAN connector 3Pin header 4RS 232 port Fig 10 Connector pin assignment on rear side Signal Explanation EV 1 Event 1 Relay contact The contact is on a floating basis Its setting depends on the settin...

Страница 28: ...ctronic trigger is send through the LAN Autozero Low active Secure switching threshold at least 10 mA A signal short circuit to ground sets the measuring sig nal to zero 24V Event controlled switching...

Страница 29: ...switched on device causes a short circuit Turn o the device before connecting cables Pull the power plug Electronic defect Electrostatic discharge can destroy the electronics Wear a protective bracel...

Страница 30: ...tor sends measuring signals from the detector Non bipolar 1 channel 0 to 5 V DAC 20 bit Scalable Adjustable to offset 4 10 Switching on The device is switched on by the power key on the back of the de...

Страница 31: ...he capillaries have been connected 5 2 Software control There are several options for controlling the device With keypad With chromatography software 5 2 1 Controlling with display and keypad The disp...

Страница 32: ...pad 5 2 3 Status LED The status of the device is indicated by an LED on the front side The color of the LED shows the current status LED Status Green Device is ready idle status Green flashing Device...

Страница 33: ...on Setting current absor bance values to zero Menu button Select main menu Return to one menu further up in the menu structure Confirmation button Confirm selection Entering sub menu Activation of num...

Страница 34: ...irmation button or use the vertical arrow keys 3 Confirm entry by using the horizontal arrow keys Note One spectral edge filter to suppress the second diffraction order is automatically activated if t...

Страница 35: ...GLP LAMPS GLP DEVICE PROGRAMS and DEVICE LINKS use the menu button and the horizontal arrow keys Navigate through main menu 1 Press to access the menu 2 Navigate between menu points by using horizonta...

Страница 36: ...30 Operation BlueShadow Detector 40D Instructions V7647 5 4 2 Menu structure overview Fig 15 Menu structure overview...

Страница 37: ...n of the detector The connections are on the rear of the device Serial interface RS232 115200 RS232 38400 RS232 19200 RS232 9600 LAN DHCP Automatic network configuration LAN manual Manual net work con...

Страница 38: ...measured for signal and reference channel P sig ref provides the ab solute light intensity of signal and reference channel at the UV maximum measured after a calibration The values are independent fr...

Страница 39: ...hreshold in the Fraction level is exceeded the event output selected in the Fraction port line is switched on and remains active until the signal drops below the threshold value The threshold always r...

Страница 40: ...gth with a scaling factor of 100 nm V This wavelength WL can then be adjusted Thus a second calibration point is generated which modifies the scaling factor Note Before operating this configuration ch...

Страница 41: ...u contains no editable parameters Menu Explanation Figure DETECTOR GLP Serial number Detector serial number Operating time Detector operating time Motor Working Grating motor operating move ment time...

Страница 42: ...ps for each program The following parameters can be defined for individual program steps Start time Wavelength Events Navigate through Program menu 1 Select the Device Programs menu 2 Use the horizont...

Страница 43: ...ed to monitor absorbance at 280 nm for the first 10 minute step and at 420 nm during the next step which has no time limit since it is the final step in this example Additionally event 1 is ena bled d...

Страница 44: ...H and press the up ward key to change the status from Hold H to Run R program starts running 5 You can again go back from Run R to hold H by pressing the downward key with the cur sor at the status s...

Страница 45: ...e the vertical arrow keys 5 6 4 Deleting a program line Process Figure 1 Select the Device Programs menu 2 Select the program number you wish to change 3 Navigate to the to be deleted program line 4 C...

Страница 46: ...d the program number you wish to start by navigating via the horizontal arrow keys and editing via the numeric pad after pressing the confirmation key or using the vertical arrow keys 4 To run the wak...

Страница 47: ...he numeric pad Confirm the selected link by using the confirmation key 3 You can view the progress of the currently run ning link in the third link display 4 After selecting a link use the horizontal...

Страница 48: ...ursor to position P P Program in the display and enter the program number you wish to run 5 Move the cursor to position R R Repeat and edit the value number of repetitions for the specified program 6...

Страница 49: ...ia the upward ar row key The detector status will switch to R Run mode 6 The link will start running the programs line by line 7 Use the downward arrow key with the cur sor on the detector status to p...

Страница 50: ...k number you wish to change 3 Navigate to the to be deleted link line e g line 02 4 Change the line number to 00 5 You will be asked whether you want to delete the link line which was active before en...

Страница 51: ...sion from our website 6 2 Operation Qualification OQ The Operation Qualification includes an extensive functionality test according to KNAUER standard OQ documents The Operation Qualification is a sta...

Страница 52: ...computer 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN...

Страница 53: ...ent incursion from interfering light or an electronics error Insert the test cell Inspect the calibration with a weak absorbing eluent Baseline noise Inspect the flow cell assembly Fasten the knurled...

Страница 54: ...delete program Cannot edit pro gram from the run ning link First pause link then edit data using the keypad Cannot initialize LAN Check cables and connections in local area net work Cannot operate an...

Страница 55: ...e program Check the detector Check the number of pro gram lines A maximum of 100 program lines are possible P Program does not exist Create a program Program is running Quit program or wait until prog...

Страница 56: ...BlueShadow Detector 40D Instructions V7647 System message Too many lines in program Check the number of program lines A maximum of 100 program lines are possible W Wrong Line number Change the entry i...

Страница 57: ...tomer Support 8 1 Maintenance contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a sepa rate maintenance contract...

Страница 58: ...Process Figure 1 Remove capillaries at the inlet 1and outlet 2of the flow cell 2 Unscrew the knurled head screws 3of the cover plate 4 Hold the flow cell securely with your hand during this procedure...

Страница 59: ...e flow cell Flow cell is removed from the detector Waste tube is connected to the flow cell The following solvents are recommended for rinsing Diluted HCl 1 mol l 1 ml l NaOH aq Ethanol Acetone Syring...

Страница 60: ...clean surface remove the compression part 2 3 The lens 3 is protected by a seal ring 4 This must be renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or w...

Страница 61: ...g 1 2 Remove the cover 2 and the spacer 3 not part of all flow cells 3 Using tweezers pull out the pressure bushing 4and the light guide 5 4 Carefully push the light guide out of the holder and strip...

Страница 62: ...uld falsify the measurement Handle the fiber optics with care and avoid impacts Move the fiber optics carefully without using pressure or bending it Detector is switched off Cap fittings for fiber opt...

Страница 63: ...sport or storage If you want to return your device to KNAUER for repairs enclose the Service Request Form which can be downloaded from our website For a secure transport note the weight and dimensions...

Страница 64: ...r classifies to category 8 and 9 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End u...

Страница 65: ...at H line FWHM Wavelength accu racy 2 5 nm Wavelength preci sion 0 3 nm ASTM E1657 98 Noise 1 5 10 5 AU at 254 nm 2 0 x 10 5 AU at 254 nm fiber optics version ASTM E1657 98 Drift 3 0 10 4 AU h at 254...

Страница 66: ...operating hours number of lamp ignitions Display Matrix keypad 11 3 General Power supply Power input 100 240 V Output 50 60 Hz Maximum power consumption 75 Watt Protection type IP 20 Dimensions W H D...

Страница 67: ...herwise Please note that higher temperatures can signifi cantly affect the stability of different materials 12 2 Plastics Polyetheretherketone PEEK PEEK is a durable and resistant plastic and next to...

Страница 68: ...ene POM POM H TF POM is a semi crystalline high molecular thermoplastic material which stands out due to its high stiffness low friction value and thermic stability It can even substitute metal in man...

Страница 69: ...terized by a high hardness a low coefficient of fric tion and thus low wear In addition it is highly biocompatible DLC is inert against all acids alkalis and solvents commonly used in HPLC Ceramic Cer...

Страница 70: ...e used or with a mixture of higher compatibility They are inert against almost all solvents Exceptions are biological appli cations which are metal ion sensible and applications with extreme cor rosiv...

Страница 71: ...Appendix Installation Qualification IQ...

Страница 72: ...cated by n a not applicable in the table Larger sections that are not used have to be crossed out diagonal line through them marked with n a and date DD MM YYYY and signed All required documents have...

Страница 73: ...transport damage No transport damage is observed 3 Check the scope of delivery The scope of delivery is complete 4 Connect the device to the power supply The device is connected 5 Connect all loose p...

Страница 74: ...o be signed by a person who is authorized by the customer s head of laboratory The scope of the IQ meets the customer s requirements The results of the IQ any changes made as well as the IQ process ha...

Страница 75: ...UER instructions online www knauer net library KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KN...

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