background image

13 

 

4.

 

Select chambers

. A screen similar to th

Figure 4

 will appear on the touch screen, and the 

dish holder will move so that the first available chamber of the GRID is in view. 

In each program, chambers that are available to be viewed and edited will depend on the program 
being run. See the section 

0

 for more details on each program. 

 

Figure 4: 

Identify

 screen showing a blank dish (user IOTA

’s

 PINK dish) before identifying monoclonal chambers. 

The chamber that is currently being viewed is indicated on the touchscreen by a blue circle - in 
the above example, the isoHub is viewing chamber A01. A larger icon to the left of the GRID 
shows the state of the chamber that is currently being viewed. 

 

All programs on the isoHub require the user to select a username and dish colour. The 
isoHub  supports  up  to  thirty-six  users.  Under  each  username  the  isoHub  can 

simultaneously track up to nine individual dishes via the dishes’ coloure

d locating rings. 

Stored user data can be managed via th

Data

 program. 

4.4

 

Chamber states 

As a dish progresses through the workflow, the Cloning Platform tracks the state of the chambers. 
The current chamber states for a dish can be viewed at any time via th

Data

 program. 

The chamber states are: 

Содержание isoHub

Страница 1: ...2 1 0 3 isoHub user manual...

Страница 2: ...e reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with...

Страница 3: ...Attach controller 10 3 2 6 Insert power cord 11 3 2 7 Attach 60mm dish holder or well plate holder 11 4 Using the isoHub 11 4 1 Powering on 11 4 2 Moving the stage 12 4 3 Running programs 12 4 4 Chamb...

Страница 4: ...phase contrast observation 21 7 1 Changing between bright field and phase contrast observation 21 7 2 Adjusting the aperture iris diaphragm 21 7 3 Selecting the objective 22 7 4 Changing between visu...

Страница 5: ...Troubleshooting 26 12 Disposal and recycling 29 12 1 Disposal of isoHub 29 12 1 1 EU Waste Electrical and Electronic Equipment WEEE Directive 29 12 1 2 Users outside the European Union 30 13 Technical...

Страница 6: ...anner not specified by the manufacturer then the protection provided by the isoHub may be impaired Powering the equipment o Use only with the supplied XP POWER VEC50US24 24VDC 50W power supply and pow...

Страница 7: ...formance and damage the isoHub o Ambient temperature 16 35 C 60 95 F o Humidity 40 60 o Altitude less than 2000m o Pollution degree 2 All services shall be performed by iotaSciences authorised personn...

Страница 8: ...7 Figure 1 Front view showing key components of the isoHub Figure 2 Back view showing key components of the isoHub...

Страница 9: ...60mm dish holder Well plate holder Dust cover Heated centre plate optional 3 2 Installation 3 2 1 Remove isoHub from packaging and place on a flat stable surface When carrying the isoHub to a differen...

Страница 10: ...nob a to the extent that it does not fall off Hold the condenser diagonally contact the attaching portion on the top surface b of the condenser to the round dovetail portion on the bottom of the colum...

Страница 11: ...ve Attach caps to screw holes a of the revolving nosepiece where no objectives are attached to avoid dust or dirt getting on the lenses After all objectives and caps are attached fit the stage centre...

Страница 12: ...c or the well plate holder into position and release points a and b Ensure the 60mm dish holder or well plate holder are flat on the stage 4 Using the isoHub 4 1 Powering on To power on the isoHub en...

Страница 13: ...dentify program can be run to review the chambers in a recently plated GRID and mark chambers containing single cells monoclonal chambers Ensure the 60mm dish holder is attached to the isoHub before s...

Страница 14: ...chscreen by a blue circle in the above example the isoHub is viewing chamber A01 A larger icon to the left of the GRID shows the state of the chamber that is currently being viewed All programs on the...

Страница 15: ...t recent run of the Extract program on the isoCell are distinguished by a small circle in their corner These chambers are the only ones that can be selected in the Prep Redetach program Inactive The c...

Страница 16: ...ee the individual sections on each program in section 0 for more details For continuous scanning of chambers press and hold the lower button on the controller How long the isoHub will remain on each c...

Страница 17: ...Speed This option will set how fast the isoHub scans through chambers when holding down the button on the joystick to jump between chambers 4 10 3 Centre If the chamber isn t centred in the field of v...

Страница 18: ...chambers that are no longer of interest can be marked as inactive and will no longer be included in the Cloning Platform workflow The isoHub will only go to filled clonal chambers to allow users to m...

Страница 19: ...n 5 4 2 Suspension cells If the dish doesn t contain any chambers that have had dissociation reagent added to them then the isoHub will move to all filled clonal chambers during the Prep Extract progr...

Страница 20: ...sing the controller Turn bed heater on or off Set the time before the isoHub goes to sleep see section 4 11 6 3 Set time The set time program more settings set time is used to update the date and time...

Страница 21: ...one isoCell isoHub isn t currently set up to sync data with any isoCells Figure 6 Data syncing status screen accessed by pressing the data syncing status icon on a menu screen The device name display...

Страница 22: ...ider within the range of 30deg Select the direction that makes it easy to observe When performing bright field observation Slide the phase contrast slider a and engage the empty hole of the slider in...

Страница 23: ...and camera light path Set the light path selection lever to the visual light path for using the eyepiece or camera light path for using a camera Visual light path 100 Camera light path 0 Visual light...

Страница 24: ...ghter the brightness becomes 8 Advanced observation 8 1 Adjusting binocular position 8 1 1 Adjusting the interpupillary distance While looking through the eyepieces move the binocular portion a until...

Страница 25: ...same manner while looking into the left eyepiece with your left eye rotate the left diopter adjustment ring b to bring the specimen into focus In this case do not touch the coarse fine focusing knobs...

Страница 26: ...n this case turn the tension adjustment ring b in the arrow direction to increase the tension of the coarse focusing knob 9 Recording with the camera The observed image can be acquired by attaching th...

Страница 27: ...ntering the isoHub may lead to degraded performance or electrical safety risk Use the provided dust cover when isoHub not in use to prevent dust 11 Troubleshooting Problem Cause Remedy The field of vi...

Страница 28: ...uorescence observation The fluorescence mirror unit is not properly engaged in the light path Engage the fluorescence mirror unit in the light path correctly Fluorescence observation The shutter for r...

Страница 29: ...scence observation The direction of the filter slider for reflected light illumination which is inserted to the fluorescence illuminator is not correct Set the direction of the filter slider for refle...

Страница 30: ...ot adjusted appropriately Adjust the tension of the coarse focusing knob to be lighter see section 8 2 The dish data on isoHub doesn t match the dish data on the isoCell The isoCell and isoHub are no...

Страница 31: ...se of your isoHub at the end of its useful life please refer to your local waste management organisation for proper disposal practices for used electronic and electrical equipment 13 Technical support...

Страница 32: ...ch of statutory duty or otherwise arising under or in connection with the purchase of this instrument for loss of profits loss of business loss of revenue loss of agreements loss of contracts loss of...

Страница 33: ...user manuals at any time without notice and the recipient is strongly encouraged to regularly check for updates on the iotaSciences customer portal The isoHub is manufactured by Iota Sciences Ltd Begb...

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