Invitrogen Bandmate MAN0018722, Руководство пользователя

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Program the processor
• Store up to 8 programs.
• All incubation steps can be set from 1 second to 96 hours.
• Volume range for washes is between 10 mL to 75 mL for mini gels and 10 mL to 150 mL for midi
gels.
• We recommend using 20-30 mL per wash for mini gels and 30-50 mL for midi gels.
• Recommended antibody volumes are ≥3.5 mL for mini blots and ≥7.5 mL for midi blots.
• Maximum number of given steps in a protocol is 9.
• Recommended rocks per minute for incubation steps is 15 rpm. Recommended rocks per minute
for wash steps is 20 rpm.
1.
From the home screen, select Programs . A list of programs, with default times will appear. The
Standard Western Blotting Protocol may be used for guidance and as a template. Select the
desired program using the dial. Press
Edit .
2.
Enter a name for the custom protocol. Select letters and numbers by turning the dial. Use the
cluster of 4 buttons at the lower left of the screen to navigate between characters and delete
characters or add spaces. When finished, press
Save to save the program name, and then
press Continue .
3.
Select whether the protocol will be used with a mini tray (two chambers with a central divider) or a
midi tray (one large chamber). Press
Save , then press Continue .
4.
Choose whether a quick rinse step is required in the protocol. A rinse will dispense 10 mL of wash
buffer at the end of each step. If a rinse is desired, highlight
Enable on the screen. Press Continue
.
Chapter 1
Bandmate
™
Automated Western Processor
Operate the Bandmate ™ Automated Western Blot Processor
1
Bandmate ™ Automated Western Blot Processor User Guide
7