Akura™ PLUS Hanging Drop System
Quick Start Guide
Thank you for choosing InSphero's Akura™ PLUS for your 3D cell culture experiments. This Quick Start
Guide contains important information to get you started immediately. For detailed instructions please
refer to the Product Manual and additional resources on
shop.insphero.com
.
Akura™ PLUS Hanging Drop System Components
A. Akura™ PLUS Plate (frame with 12x8 well
strips) + humidifier pads (in bags with
tweezer)
B. Transparent lid
C. Bottom plate with reservoir
D. Akura™ 96 well plate
Generating Spheroids or Organoids
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Figure 1. Akura™ PLUS system components.
Akura™ PLUS
1.
Preparation:
Prepare a reservoir with 20ml 0.5x PBS, remove one of the humidifier pads using the
tweezer and place it in the reservoir. Wait approx. 5 min until the pad is completely soaked with PBS and
place it in the bottom plate of
the Akura™ PLUS Plate.
2.
Cell seeding:
Count
the cells and prepare a cell
suspension for seeding, using
a final volume per drop of
40µl. For long-term growth
profiling start with low cell
numbers (250 – 500 cells per
drop). For non-proliferating
cells or rapid production of
larger spheroids start with
higher numbers (from 2,500
cells per drop). Please try
different concentrations for
defining your optimal range.
Ensure tight contact
for SureDrop
TM
inlet
Akura
TM
PLUS
Akura
TM
96 Plate
(Spheroid Culture Platform)
Spheroid
culture/assay
Spheroid
transfer
Spheroid
maturation
Cell seeding
!
(Spheroid Formation
Platform)
Figure 2: Delivering cell suspensions
and generating spheroids in Akura™
PLUS Hanging Drop System.
