Concentrating Pipette
Quick Start Guide
(US Patents 8,110,112, 8,584,535, D688,805
and other patents pending)
Important notes:
Ensure the quick connect fitting on the permeate tube is fully seated in its
Permeate Port
on the side of the instrument;
otherwise the fluid will not flow through the instrument. Clip the fluid line to the container to keep it from coming out.
The instrument can concentrate and extract with the
Fluidics Head
in any position.
Never run alcohol through the instrument as it may damage internal components.
Use the
Arm Tension Knob
on the left side of the arm to adjust how tightly the
Fluidics Head
is held in place.
Use the up (
▲
) and down (
▼
) arrows to highlight options on the
Control Panel
and press enter (
↵
) to select. To adjust
settings, press enter and use the up and down keys to select the value desired. Then press the enter key again. At the
bottom of each menu tree, the option “Return” is displayed; selecting this option will return you to the previous menu.
A run can be paused at any time by pressing enter. You will be able to restart the run as long as no air is allowed to enter
the
Single-use Concentrating Pipette Tip
. Selecting “End Run” on the pause menu will allow you to perform an extraction.
The final volume of your sample is controlled by the extraction fluid valve open time. Generic “small”, “med.” and “large”
extraction fluid volume values are found in the “Options”/”Ext. size” menu, however under “Adv. Options” you can
precisely control the number of milliseconds the valve is open, as well as the number of pulses the valve performs. The
longer the open time and the more pulses performed, the larger the extraction volume. For reference, a single 75 msec
pulse will produce approximately a 100 µL extraction volume.
You can perform a test extraction by selecting “Extract” in the “Procedures” menu.
When adjusting the extraction volume, be sure to perform the test extraction with a tip installed, otherwise you will not
account for the volume of elution fluid that remains in the tip at the end of an extraction cycle.
Be sure that the tip is firmly “clicked” into position; it will snap into the detent indicating that it is fully seated.
Once a
Single-use Concentrating Pipette Tip
has been used to concentrate a sample it cannot be reused. Keep a used tip
handy to test extraction volume settings without wetting a new tip.
Protect the ‘Can Interface Port’ from contaminants by covering when not in use to or by keeping a can in place. The port
should be wiped with alcohol before use.
Refer to the chemical compatibility chart in the user manual.
