illumina HiSeq X, Руководство пользователя, Страница: 69 / 86

R
e
a
l-T
im
e
A
n
a
lysi
s
W
o
rk
flo
w
HiSeq X System User Guide
59
Quality scoring calculates a set of predictors for each base call, and then uses the predictor
values to look up the Q-score in a quality table. Quality tables are created to provide
optimally accurate quality predictions for runs generated by a specific configuration of
sequencing platform and version of chemistry.
After the Q-score is determined, results are recorded in base call (*.bcl) files. For more
information, see
Sequencing Output Files on page 60 .
Q-Score Binning
RTA v2 groups quality scores into specific ranges, or bins, and assigns a value to each
range. Q-score binning significantly reduces storage space requirements without affecting
accuracy or performance of downstream applications.
Q-score binning contributes to the efficiency of analysis processes and data transfer
requirements associated with the high throughput of the HiSeq X. The resulting *.bcl file is
smaller because the compression algorithms are able to compress the file more effectively.
Less data are written to the instrument computer and transferred to a network location,
making the file copy faster.