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42
Part # 15050091 Rev. C
Perform a Maintenance Wash
A maintenance wash is required every 10 days.
After each sequencing run, an instrument wash is required. You have the option to perform
a maintenance wash or a water wash after a run. Illumina recommends a maintenance
wash after each run.
NOTE
Using HiSeq X Control Software v3.1, or later, a maintenance wash is recommended after
each run, but no longer required. Additionally, gasket replacement is no longer required
with every maintenance wash; instead replace gaskets before starting the required
maintenance wash every 10 days.
If the Load Gasket screen opens before a maintenance wash, replace the 8-port gaskets in
the front manifold and back manifold before proceeding to the wash.
The maintenance wash consists of 3 wash steps:
}
First water wash
—Flushes the system with laboratory-grade water.
}
NaOH wash
—Washes the system with NaOH.
}
Final water wash
—Flushes the system with laboratory-grade water to remove NaOH.
Positions
Wash Time
8 SBS positions
20 minutes
8 SBS positions and 10 paired-end positions
60 minutes
User-Supplied Consumables
}
Ethanol wipes
}
24 bottles, 250 ml (Corning, catalog # 430776), 8 bottles per wash step
}
30 tubes, 15 ml (Corning, catalog # 430052), 10 tubes per wash step
}
Laboratory-grade water
}
1 N NaOH
First Water Wash
1
From the Welcome screen, select
Wash | Maintenance
.
2
Select
Yes
to wash paired-end reagent positions. Otherwise, select
No
to wash
SBS reagent positions only. Select
Next
to proceed.