illumina HiSeq X, Руководство пользователя, Страница: 36 / 86

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Part # 15050091 Rev. C
7 Place each reagent bottle onto the rack in the associated numbered position. Make sure
that the conical end of the bottle rests in the indentation on the base of the rack.
Position Reagent Description
1
PIM Patterned Incorporation Mix
2
PW1 25 ml of PW1 or laboratory-grade water
3
PSM Patterned Scan Mix
4
PB1 Patterned SBS Buffer 1
5
PB2 Patterned SBS Buffer 2
6
PB2 Patterned SBS Buffer 2
7
PCM Patterned Cleavage Mix
8
PB2 Patterned SBS Buffer 2
Table 1 SBS Reagent Positions
8 Add 25 ml of PW1 or laboratory-grade water to the bottle in position 2.
9 Slide the reagent rack into the reagent compartment, aligning the rack with the raised
guide on the floor of the compartment.
10 Lower the sippers into the sequencing reagent bottles as follows:
a Pull the sipper handle towards you and then lower the sipper handle.
b Visually inspect the sippers to make sure that they do not bend as they lower into
the funnel caps.
c Release the sipper handle into the slot on the bottom end of the groove.
11 Select the PW1 (25 ml) loaded in Position 2 checkbox.
Load Indexing and Paired-End Reagents
1 Record the weight of each reagent on the lab tracking form.
2 Raise the sippers for the paired-end reagent rack using the following motion:
a Pull the handle towards you and then raise the handle.
b Release the handle into the slot on the top end of the groove. Make sure that the
handle rests securely in the slot.
3 Slide the reagent rack out of the reagent compartment using the rack handle.
4 Place 15 ml conical tubes filled with 10 ml PW1 or laboratory-grade water in positions
12, 18, and 19 on the paired-end rack.